Apobec3 (NM_030255) Mouse Untagged Clone

CAT#: MC211919

Apobec3 (untagged) - Mouse apolipoprotein B mRNA editing enzyme, catalytic polypeptide 3 (Apobec3), transcript variant 2, (10ug)



  "NM_030255" in other vectors (4)

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
APOBEC3 Antibody - middle region
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Apobec; Arp3; BC003314; Cem15; Rfv3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC211919 representing NM_030255
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGACCATTCTGTCTGGGATGCAGCCATCGCAAATGCTATTCACCGATCAGAAACCTGATATCTCAAG
AAACATTCAAGTTCCACTTTAAGAACCTAGGCTATGCCAAAGGCCGGAAAGATACCTTCTTGTGCTATGA
AGTGACTAGAAAGGACTGCGATTCACCCGTCTCCCTTCACCATGGGGTCTTTAAGAACAAGGACAACATC
CACGCTGAAATCTGCTTTTTATACTGGTTCCATGACAAAGTACTGAAAGTGCTGTCTCCGAGAGAAGAGT
TCAAGATCACCTGGTATATGTCCTGGAGCCCCTGTTTCGAATGTGCAGAGCAGATAGTAAGGTTCCTGGC
TACACACCACAACCTGAGCCTGGACATCTTCAGCTCCCGCCTCTACAACGTACAGGACCCAGAAACCCAG
CAGAATCTTTGCAGGCTGGTTCAGGAAGGAGCCCAGGTGGCTGCCATGGACCTATACGAATTTAAAAAGT
GTTGGAAGAAGTTTGTGGACAATGGTGGCAGGCGATTCAGGCCTTGGAAAAGACTGCTTACAAATTTTAG
ATACCAGGATTCTAAGCTTCAGGAGATTCTGAGGCGAATGGACCCGCTAAGTGAAGAGGAATTTTACTCG
CAGTTTTACAACCAACGAGTCAAGCATCTCTGCTACTACCACCGCATGAAGCCCTATCTATGCTACCAGC
TGGAGCAGTTCAATGGCCAAGCGCCACTCAAAGGCTGCCTGCTAAGCGAGAAAGGCAAACAGCATGCAGA
AATCCTCTTCCTTGATAAGATTCGGTCCATGGAGCTGAGCCAAGTGACAATCACCTGCTACCTCACCTGG
AGCCCCTGCCCAAACTGTGCCTGGCAACTGGCGGCATTCAAAAGGGATCGTCCAGATCTAATTCTGCATA
TCTACACCTCCCGCCTGTATTTCCACTGGAAGAGGCCCTTCCAGAAGGGGCTGTGTTCTCTGTGGCAATC
AGGGATCCTGGTGGACGTCATGGACCTCCCACAGTTTACTGACTGCTGGACAAACTTTGTGAACCCGAAA
AGGCCGTTTTGGCCATGGAAAGGATTGGAGATAATCAGCAGGCGCACACAAAGGCGGCTCCGCAGGATCA
AGGAGTCCTGGGGTCTGCAAGATTTGGTGAATGACTTTGGAAACCTACAGCTTGGACCCCCGATGTCTTG
A


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_030255
Insert Size 1191 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_030255.3, NP_084531.2
RefSeq Size 2360 bp
RefSeq ORF 1191 bp
Locus ID 80287
Gene Summary DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single-or double-stranded RNA. Exhibits antiviral activity against HIV-1, simian immunodeficiency viruses (SIVs), mouse mammary tumor virus (MMTV) and friend murine leukemia virus (FrMLV) and may inhibit the mobility of LTR retrotransposons.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) lacks an alternate in-frame exon in the coding region, compared to variant 1. The encoded isoform (2)is shorter than isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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