Neil2 (NM_201610) Mouse Untagged Clone

CAT#: MC214776

Neil2 (untagged) - Mouse nei like 2 (E. coli) (Neil2), (10ug)



  "NM_201610" in other vectors (4)

CNY 3,600.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Gm1212; NEH2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC214776 representing NM_201610
Red=Cloning site Blue=ORF

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCAGAAGGGCCATCTGTGAGGAAGTTTCACCATCTTGTCTCCCCCTTTGTGGGCCAGAAGGTGGTCA
AGACGGGGGGCAGCAGTAAGAAGCTCCACCCTGCCGCCTTTCAGTCTCTGTGGCTCCAGGATGCTCAGGT
GCATGGAAAAAAATTATTCCTTCGGTTTGATCCAGATGAGGAGATGGAGCCACTCAACAGCAGCCCACAG
CCTATACAGGGAATGTGGCAGAAAGAGGCTGTGGACCGAGAGCTGGCCTTGGGTCCCAGTGCTCAGGAAC
CCTCTGCAGGTCCCTCTGGATCTGGGGAGCCTGTTCCCAGTAGATCTGCTGAAACATATAATCTTGGGAA
GATCCCCTCAGCAGATGCCCAGAGGTGGCTGGAGGTCAGGTTTGGTTTATTTGGCAGTATCTGGGTGAAT
GACTTCTCCAGAGCAAAGAAAGCTAACAAAAAAGGTGACTGGAGAGACCCAGTGCCCAGGCTGGTACTCC
ATTTTAGTGGTGGTGGCTTCCTGGTATTTTATAACTGCCAGATGTCATGGAGCCCTCCCCCAGTGATTGA
GCCCACCTGTGACATATTGTCTGAAAAGTTCCATCGAGGACAAGCCTTGGAAGCTCTAAGCCAGGCTCAG
CCTGTGTGCTACACACTCTTGGACCAGAGATACTTCTCAGGATTAGGGAACATCATAAAGAATGAAGCCT
TGTACAGAGCAAGGATCCATCCCCTCTCTCTCGGTTCATGCCTGAGTTCTTCCTCTCGGGAGGCCCTCGT
GGATCACGTGGTGGAGTTCAGTAAGGACTGGCTTCGGGACAAATTCCAAGGCAAGGAACGGCACACACAG
ATCTACCAGAAGGAACAATGTCCTTCTGGTCACCAGGTCATGAAGGAGACATTTGGGCCCCCAGATGGGC
TCCAGAGGCTCACCTGGTGGTGCCCTCAATGCCAGCCCCAGCTGTCCTCCAAGGGGCCCCAGAATCTCCC
GTCCTCCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_201610
Insert Size 990 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_201610.2, NP_963904.2
RefSeq Size 1914 bp
RefSeq ORF 990 bp
Locus ID 382913
UniProt ID Q6R2P8
Gene Summary Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Has DNA glycosylase activity towards 5-hydroxyuracil and other oxidized derivatives of cytosine with a preference for mismatched double-stranded DNA (DNA bubbles). Has low or no DNA glycosylase activity towards thymine glycol, 2-hydroxyadenine, hypoxanthine and 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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