Neil2 (NM_201610) Mouse Untagged Clone
CAT#: MC214776
Neil2 (untagged) - Mouse nei like 2 (E. coli) (Neil2), (10ug)
CNY 3,600.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | Gm1212; NEH2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC214776 representing NM_201610
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGAAGGGCCATCTGTGAGGAAGTTTCACCATCTTGTCTCCCCCTTTGTGGGCCAGAAGGTGGTCA AGACGGGGGGCAGCAGTAAGAAGCTCCACCCTGCCGCCTTTCAGTCTCTGTGGCTCCAGGATGCTCAGGT GCATGGAAAAAAATTATTCCTTCGGTTTGATCCAGATGAGGAGATGGAGCCACTCAACAGCAGCCCACAG CCTATACAGGGAATGTGGCAGAAAGAGGCTGTGGACCGAGAGCTGGCCTTGGGTCCCAGTGCTCAGGAAC CCTCTGCAGGTCCCTCTGGATCTGGGGAGCCTGTTCCCAGTAGATCTGCTGAAACATATAATCTTGGGAA GATCCCCTCAGCAGATGCCCAGAGGTGGCTGGAGGTCAGGTTTGGTTTATTTGGCAGTATCTGGGTGAAT GACTTCTCCAGAGCAAAGAAAGCTAACAAAAAAGGTGACTGGAGAGACCCAGTGCCCAGGCTGGTACTCC ATTTTAGTGGTGGTGGCTTCCTGGTATTTTATAACTGCCAGATGTCATGGAGCCCTCCCCCAGTGATTGA GCCCACCTGTGACATATTGTCTGAAAAGTTCCATCGAGGACAAGCCTTGGAAGCTCTAAGCCAGGCTCAG CCTGTGTGCTACACACTCTTGGACCAGAGATACTTCTCAGGATTAGGGAACATCATAAAGAATGAAGCCT TGTACAGAGCAAGGATCCATCCCCTCTCTCTCGGTTCATGCCTGAGTTCTTCCTCTCGGGAGGCCCTCGT GGATCACGTGGTGGAGTTCAGTAAGGACTGGCTTCGGGACAAATTCCAAGGCAAGGAACGGCACACACAG ATCTACCAGAAGGAACAATGTCCTTCTGGTCACCAGGTCATGAAGGAGACATTTGGGCCCCCAGATGGGC TCCAGAGGCTCACCTGGTGGTGCCCTCAATGCCAGCCCCAGCTGTCCTCCAAGGGGCCCCAGAATCTCCC GTCCTCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI |
ACCN | NM_201610 |
Insert Size | 990 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_201610.2, NP_963904.2 |
RefSeq Size | 1914 bp |
RefSeq ORF | 990 bp |
Locus ID | 382913 |
UniProt ID | Q6R2P8 |
Gene Summary | Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Has DNA glycosylase activity towards 5-hydroxyuracil and other oxidized derivatives of cytosine with a preference for mismatched double-stranded DNA (DNA bubbles). Has low or no DNA glycosylase activity towards thymine glycol, 2-hydroxyadenine, hypoxanthine and 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG220912 | Neil2 (tGFP-tagged) - Mouse nei like 2 (E. coli) (Neil2), (10ug) |
CNY 4,370.00 |
|
MR220912 | Neil2 (Myc-DDK-tagged) - Mouse nei like 2 (E. coli) (Neil2) |
CNY 3,600.00 |
|
MR220912L3 | Lenti ORF clone of Neil2 (Myc-DDK-tagged) - Mouse nei like 2 (E. coli) (Neil2) |
CNY 5,890.00 |
|
MR220912L4 | Lenti ORF clone of Neil2 (mGFP-tagged) - Mouse nei like 2 (E. coli) (Neil2) |
CNY 5,890.00 |