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Dph1 (NM_144491) Mouse Untagged Clone
CAT＃: MC215860
Dph1 (untagged) - Mouse DPH1 homolog (S. cerevisiae) (Dph1), (10ug)
CNY 5,700.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 2310011M22Rik; 4930488F09Rik; AW551873; Dph2l1; Ovca1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC215860 representing NM_144491
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGCTGGTTGTGTCCGAGACTGCGGAGCCAGGAAGCCGAGTCGGCCCTGGCAGAGGTCGCATCT CTCGGGGGCGACTGGCCAATCAGATCCCCCCTGAGGTCCTGAACAACCCCCAGTTACAGGCTGCTGTCCA AGTTCTGCCTTCTAACTACAACTTTGAGATCCCCAAAACCATCTGGAGAATCCAGCAGGCCCAGGCCAAG AAGGTGGCCTTACAAATGCCAGAAGGCCTCCTCCTCTTTGCCTGCACTATTGTGGATATCTTGGAAAGGT TCACAGAGGCTGAGGTGATGGTGATGGGTGATGTCACCTATGGGGCTTGCTGTGTGGATGACTTCACTGC AAGGGCCTTGGGAGTTGACTTCCTGGTGCACTATGGTCACAGCTGTCTAGTCCCCATGGACACCTCCGTT CAAGACTTCCGAGTCTTGTATGTCTTCGTGGATATCCGGATAGACACTGCCCACCTTCTGGACTCGGTCC GCCTCACCTTTACCCCAGGCAGCTCACTCGCTCTGGTCAGCACCATTCAGTTTGTGTCAACCTTACAGGC AGCTGCACAGGAGCTGAAAGCTGATTATCACATCAGTGTCCCACAGTGCAAGCCCCTGTCCCCTGGGGAG ATCCTAGGCTGCACATCCCCTCGGCTATCCAAGGAAGTGGAAGCTGTTGTGTATCTTGGAGATGGCCGCT TTCATCTGGAGTCTGTCATGATTGCCAACCCTAATATACCTGCTTACCGGTATGACCCATATGGCAAAGT CCTATCCAGAGAATACTATGACCATCAGCGCATGCAGGCCACTCGCCAGGAAGCCATTGCTGCTGCACGC TCAGCCAAATCCTGGGGCCTTATTCTGGGAACCTTGGGCCGCCAGGGCAGTCCCAAGATCCTGGAGCACT TGGAATCTCAGCTCAGAAACTTGGGACTTCCTTTCGTGAGGCTGTTGCTCTCTGAGATCTTCCCCAGCAA GCTCAGTCTACTTCCTGAGGTGGATGTGTGGGTGCAGGTGGCATGTCCACGCCTCTCCATTGACTGGGGT TCAGCCTTTCCCAAGCCACTGCTGACACCGTACGAGGCAGCTGTGGCCCTGAAGGACATTTCTTGGCAGC AACCCTACCCCATGGACTTCTACTCTGGCAGCTCCTTAGGGCCATGGACAGTGAACTACGGTCGGGACCG AGCACCTCGGGGTCTCTGCCAGCCTGCATCTGACAAGGTGCAGCAAGGGTCCAGAGGCGGCTCTCCAGCC CCAGCCTGTGAGAGTTGCAACTGCGCAGACCAGAAGGCTACTTCGCCGGCTCCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_144491 |
| Insert Size | 1317 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_144491.2, NP_652762.2 |
| RefSeq Size | 2103 bp |
| RefSeq ORF | 1317 bp |
| Locus ID | 116905 |
| UniProt ID | Q5NCQ5 |
| Gene Summary | Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2). Acts also as a tumor suppressor in lung and breast cancers. Plays a role in embryonic growth, organogenesis and postnatal survival. When overexpressed, suppresses colony formation ability and growth rate of ovarian cancer cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG216667 | Dph1 (tGFP-tagged) - Mouse DPH1 homolog (S. cerevisiae) (Dph1), (10ug) |
CNY 4,280.00 |
|
| MR216667 | Dph1 (Myc-DDK-tagged) - Mouse DPH1 homolog (S. cerevisiae) (Dph1) |
CNY 3,656.00 |
|
| MR216667L3 | Lenti ORF clone of Dph1 (Myc-DDK-tagged) - Mouse DPH1 homolog (S. cerevisiae) (Dph1) |
CNY 5,800.00 |
|
| MR216667L4 | Lenti ORF clone of Dph1 (mGFP-tagged) - Mouse DPH1 homolog (S. cerevisiae) (Dph1) |
CNY 5,800.00 |
