Dph1 (NM_144491) Mouse Tagged ORF Clone
CAT#: MR216667
- TrueORF®
Dph1 (Myc-DDK-tagged) - Mouse DPH1 homolog (S. cerevisiae) (Dph1)
ORF Plasmid: tGFP
"NM_144491" in other vectors (4)
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CNY 3,656.00
CNY 3,900.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2310011M22Rik; 4930488F09Rik; AW551873; Dph2l1; Ovca1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR216667 representing NM_144491
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGCTGGTTGTGTCCGAGACTGCGGAGCCAGGAAGCCGAGTCGGCCCTGGCAGAGGTCGCATCT CTCGGGGGCGACTGGCCAATCAGATCCCCCCTGAGGTCCTGAACAACCCCCAGTTACAGGCTGCTGTCCA AGTTCTGCCTTCTAACTACAACTTTGAGATCCCCAAAACCATCTGGAGAATCCAGCAGGCCCAGGCCAAG AAGGTGGCCTTACAAATGCCAGAAGGCCTCCTCCTCTTTGCCTGCACTATTGTGGATATCTTGGAAAGGT TCACAGAGGCTGAGGTGATGGTGATGGGTGATGTCACCTATGGGGCTTGCTGTGTGGATGACTTCACTGC AAGGGCCTTGGGAGTTGACTTCCTGGTGCACTATGGTCACAGCTGTCTAGTCCCCATGGACACCTCCGTT CAAGACTTCCGAGTCTTGTATGTCTTCGTGGATATCCGGATAGACACTGCCCACCTTCTGGACTCGGTCC GCCTCACCTTTACCCCAGGCAGCTCACTCGCTCTGGTCAGCACCATTCAGTTTGTGTCAACCTTACAGGC AGCTGCACAGGAGCTGAAAGCTGATTATCACATCAGTGTCCCACAGTGCAAGCCCCTGTCCCCTGGGGAG ATCCTAGGCTGCACATCCCCTCGGCTATCCAAGGAAGTGGAAGCTGTTGTGTATCTTGGAGATGGCCGCT TTCATCTGGAGTCTGTCATGATTGCCAACCCTAATATACCTGCTTACCGGTATGACCCATATGGCAAAGT CCTATCCAGAGAATACTATGACCATCAGCGCATGCAGGCCACTCGCCAGGAAGCCATTGCTGCTGCACGC TCAGCCAAATCCTGGGGCCTTATTCTGGGAACCTTGGGCCGCCAGGGCAGTCCCAAGATCCTGGAGCACT TGGAATCTCAGCTCAGAAACTTGGGACTTCCTTTCGTGAGGCTGTTGCTCTCTGAGATCTTCCCCAGCAA GCTCAGTCTACTTCCTGAGGTGGATGTGTGGGTGCAGGTGGCATGTCCACGCCTCTCCATTGACTGGGGT TCAGCCTTTCCCAAGCCACTGCTGACACCGTACGAGGCAGCTGTGGCCCTGAAGGACATTTCTTGGCAGC AACCCTACCCCATGGACTTCTACTCTGGCAGCTCCTTAGGGCCATGGACAGTGAACTACGGTCGGGACCG AGCACCTCGGGGTCTCTGCCAGCCTGCATCTGACAAGGTGCAGCAAGGGTCCAGAGGCGGCTCTCCAGCC CCAGCCTGTGAGAGTTGCAACTGCGCAGACCAGAAGGCTACTTCGCCGGCTCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216667 representing NM_144491
Red=Cloning site Green=Tags(s) MAALVVSETAEPGSRVGPGRGRISRGRLANQIPPEVLNNPQLQAAVQVLPSNYNFEIPKTIWRIQQAQAK KVALQMPEGLLLFACTIVDILERFTEAEVMVMGDVTYGACCVDDFTARALGVDFLVHYGHSCLVPMDTSV QDFRVLYVFVDIRIDTAHLLDSVRLTFTPGSSLALVSTIQFVSTLQAAAQELKADYHISVPQCKPLSPGE ILGCTSPRLSKEVEAVVYLGDGRFHLESVMIANPNIPAYRYDPYGKVLSREYYDHQRMQATRQEAIAAAR SAKSWGLILGTLGRQGSPKILEHLESQLRNLGLPFVRLLLSEIFPSKLSLLPEVDVWVQVACPRLSIDWG SAFPKPLLTPYEAAVALKDISWQQPYPMDFYSGSSLGPWTVNYGRDRAPRGLCQPASDKVQQGSRGGSPA PACESCNCADQKATSPAP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_144491 |
ORF Size | 1314 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_144491.2, NP_652762.2 |
RefSeq Size | 2103 bp |
RefSeq ORF | 1317 bp |
Locus ID | 116905 |
UniProt ID | Q5NCQ5 |
MW | 48.5 kDa |
Gene Summary | Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2). Acts also as a tumor suppressor in lung and breast cancers. Plays a role in embryonic growth, organogenesis and postnatal survival. When overexpressed, suppresses colony formation ability and growth rate of ovarian cancer cells (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC215860 | Dph1 (untagged) - Mouse DPH1 homolog (S. cerevisiae) (Dph1), (10ug) |
CNY 5,700.00 |
|
MG216667 | Dph1 (tGFP-tagged) - Mouse DPH1 homolog (S. cerevisiae) (Dph1), (10ug) |
CNY 4,280.00 |
|
MR216667L3 | Lenti ORF clone of Dph1 (Myc-DDK-tagged) - Mouse DPH1 homolog (S. cerevisiae) (Dph1) |
CNY 5,800.00 |
|
MR216667L4 | Lenti ORF clone of Dph1 (mGFP-tagged) - Mouse DPH1 homolog (S. cerevisiae) (Dph1) |
CNY 5,800.00 |