Htr7 (NM_008315) Mouse Untagged Clone

CAT#: MC215943

Htr7 (untagged) - Mouse 5-hydroxytryptamine (serotonin) receptor 7 (Htr7), (10ug)



  "NM_008315" in other vectors (4)

CNY 5,800.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 5-HT7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC215943 representing NM_008315
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGACGTTAACAGCAGCGGCCGCCCCGACCTCTACGGCCATCTCCGCTCTCTCATCCTGCCGGAGG
TGGGGCGCAGGCTGCAGGACCTGAGCCCCGACGGTGGCGCCCACTCGGTGGTGAGCTCCTGGATGCCGCA
CCTGCTGAGCGGCTTCCCAGAGGTGACAGCTAGCCCGGCGCCCACCTGGGACGCGCCCCCGGACAATGTC
TCCGGCTGCGGGGAGCAGATCAACTATGGCAGAGTCGAGAAAGTTGTGATCGGCTCCATCCTGACGCTCA
TCACGCTGCTGACGATCGCGGGCAACTGCCTGGTGGTGATCTCGGTGTGCTTTGTCAAGAAGCTCCGCCA
GCCCTCCAACTACCTGATTGTGTCCCTGGCGCTGGCTGACCTCTCGGTGGCCGTGGCGGTCATGCCTTTC
GTTAGTGTCACGGACCTCATCGGGGGCAAGTGGATCTTCGGCCACTTCTTCTGCAACGTCTTCATCGCCA
TGGACGTCATGTGCTGCACGGCCTCGATCATGACCCTGTGCGTGATCAGCATCGACAGGTACCTTGGGAT
CACCAGGCCCCTCACGTACCCCGTGAGGCAGAATGGGAAATGTATGGCCAAAATGATTCTGTCGGTCTGG
CTTCTCTCTGCCTCCATCACCTTACCTCCTCTCTTCGGATGGGCTCAGAATGTGAACGATGACAAAGTGT
GCTTGATCAGCCAGGATTTTGGCTACACGATCTACTCCACCGCAGTGGCGTTTTATATCCCCATGTCGGT
CATGCTGTTCATGTACTATCAGATTTACAAGGCCGCCAGGAAGAGCGCAGCCAAACACAAGTTCTCAGGC
TTCCCACGCGTGCAGCCGGAGAGCGTCATCTCCCTGAATGGCGTGGTGAAACTCCAGAAGGAGGTGGAAG
AGTGTGCGAACCTTTCGAGACTGCTCAAACACGAAAGGAAAAACATTTCCATCTTCAAGAGGGAACAGAA
AGCAGCCACTACCTTGGGGATCATCGTGGGAGCCTTCACGGTGTGCTGGCTGCCGTTTTTCCTCTTGTCC
ACAGCAAGGCCCTTTATCTGTGGCACCTCCTGTAGCTGCATCCCGCTGTGGGTGGAGAGGACATGTCTGT
GGCTGGGCTATGCAAACTCTCTCATTAACCCTTTTATATATGCCTTCTTCAACCGGGACCTGAGGACCAC
CTATCGTAGCCTACTCCAGTGCCAGTACCGGAATATCAACCGGAAGCTCTCTGCTGCAGGCATGCACGAA
GCCCTGAAACTTGCTGAGAGGCCTGAGAGAAGCGAGTTTGTGCTACAAAACTGTGACCACTGTGGGAAAA
AAGGTCATGATACATGA


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII     
ACCN NM_008315
Insert Size 1347 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_008315.2, NP_032341.2
RefSeq Size 3095 bp
RefSeq ORF 1347 bp
Locus ID 15566
UniProt ID P32304
Gene Summary This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1, also known as 5-HT7a) lacks an alternate exon in the 3' coding region, compared to variant 2. The encoded isoform (1) is shorter and has a distinct C-terminus, compared to isoform 2. 5-HT7a cited in PMID 18793707. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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