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Pik3r1 (NM_001024955) Mouse Untagged Clone
CAT＃: MC216127
Pik3r1 (untagged) - Mouse phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) (Pik3r1), transcript variant 1, (10ug)
CNY 5,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | p50alpha; p55alpha; p85alpha; PI3K |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC216127 representing NM_001024955
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACACCACGGTTTGGACTATGGAAGACCTGGACTTAGAGTGTGCCAAGACAGATATAAACTGTGGCA CAGACTTGGTGTTTTATATAGAAATGGATCCACCAGCACTGCCCCCCAAACCACCCAAGCCCACTACTGT AGCCAACAACAGCATGAACAACAATATGTCCTTGCAGGATGCTGAATGGTACTGGGGAGACATCTCAAGG GAAGAAGTGAATGAAAAACTCCGAGACACTGCTGATGGGACCTTTTTGGTACGAGACGCATCTACTAAAA TGCACGGCGATTACACTCTTACACTAAGGAAAGGAGGAAATAACAAATTAATCAAAATCTTTCACCGTGA TGGAAAATATGGCTTCTCTGATCCATTAACCTTCAACTCTGTGGTTGAGTTAATAAACCACTACCGGAAT GAGTCTTTAGCTCAGTACAACCCCAAGCTGGATGTGAAGTTGCTCTACCCAGTGTCCAAATACCAGCAGG ATCAAGTTGTCAAAGAAGATAATATTGAAGCTGTAGGGAAAAAATTACATGAATATAATACTCAATTTCA AGAAAAAAGTCGGGAATATGATAGATTATATGAGGAGTACACCCGTACTTCCCAGGAAATCCAAATGAAA AGAACGGCTATCGAAGCATTTAATGAAACCATAAAAATATTTGAAGAACAATGCCAAACCCAGGAGCGGT ACAGCAAAGAATACATAGAGAAGTTTAAACGCGAAGGCAACGAGAAAGAAATTCAAAGGATTATGCATAA CCATGATAAGCTGAAGTCGCGTATCAGTGAGATCATTGACAGTAGGAGGAGGTTGGAAGAAGACTTGAAG AAGCAGGCAGCTGAGTACCGAGAGATCGACAAACGCATGAACAGTATTAAGCCGGACCTCATCCAGTTGA GAAAGACAAGAGACCAATACTTGATGTGGCTGACGCAGAAAGGTGTGCGGCAGAAGAAGCTGAACGAGTG GCTGGGGAATGAAAATACCGAAGATCAATACTCCCTGGTAGAAGATGATGAGGATTTGCCCCACCATGAC GAGAAGACGTGGAATGTCGGGAGCAGCAACCGAAACAAAGCGGAGAACCTATTGCGAGGGAAGCGAGACG GCACTTTCCTTGTCCGGGAGAGCAGTAAGCAGGGCTGCTATGCCTGCTCCGTAGTGGTAGACGGCGAAGT CAAGCATTGCGTCATTAACAAGACTGCCACCGGCTATGGCTTTGCCGAGCCCTACAACCTGTACAGCTCC CTGAAGGAGCTGGTGCTACATTATCAACACACCTCCCTCGTGCAGCACAATGACTCCCTCAATGTCACAC TAGCATACCCAGTATATGCACAACAGAGGCGATGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001024955 |
| Insert Size | 1365 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001024955.2, NP_001020126.1 |
| RefSeq Size | 5839 bp |
| RefSeq ORF | 1365 bp |
| Locus ID | 18708 |
| Gene Summary | Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (By similarity). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress- and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348926).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) lacks a portion of the 5' coding region and initiates translation at an alternate start codon, compared to variant 2. The encoded isoform (1) has a shorter and distinct N-terminus, compared to isoform 2. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226192 | Pik3r1 (tGFP-tagged) - Mouse phosphatidylinositol 3-kinase regulatory subunit polypeptide 1 (p85 alpha) (Pik3r1) transcript variant 1, (10ug) |
CNY 7,456.00 |
|
| MR226192 | Pik3r1 (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) (Pik3r1), transcript variant 1 |
CNY 4,560.00 |
|
| MR226192L3 | Lenti ORF clone of Pik3r1 (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) (Pik3r1), transcript variant 1 |
CNY 6,460.00 |
|
| MR226192L4 | Lenti ORF clone of Pik3r1 (mGFP-tagged) - Mouse phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha) (Pik3r1), transcript variant 1 |
CNY 6,460.00 |
