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Rgma (NM_177740) Mouse Untagged Clone
CAT＃: MC216203
Rgma (untagged) - Mouse RGM domain family, member A (Rgma), (10ug)
CNY 5,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | BC059072; C230063O06 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC216203 representing NM_177740
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCGCCAAGGGAGAGGCTAGTGGTAACAGGCCGAGCTGGATGGATGGGTATGGGGAGAGGGGCAG GACGTTCAGCCCTGGGATTGTGGCCGACCCTCGCCTTCCTTCTCTGCAGCTTCCCCGCAGCCATCTCCCC CTGCAAAATCCTCAAGTGCAACTCTGAGTTCTGGAGCGCCACGTCATCAGGCAGCCACGCCCCTGCCTCC GACGACGTGCCGGAGTTCTGTGCAGCCCTGCGCACCTACGCCCTGTGCACTCGGCGGACAGCCCGCACCT GCCGGGGCGACCTGGCTTACCACTCGGCTGTCCATGGCATAGAGGACCTTATGAGCCAGCACAACTGCTC CAAGGACGGCCCCACCTCACAGCCGCGAGTGCGCACGCTCCCGCCAGCTGGGGACAGCCAGGAGCGCTCG GATAGCCCCGAGATCTGCCACTATGAGAAGAGTTTCCACAAGCACTCAGCTGCCCCCAACTACACTCACT GCGGCCTCTTTGGGGACCCACACCTCAGGACTTTCACAGACCACTTCCAGACATGCAAGGTGCAAGGCGC CTGGCCTCTCATCGACAATAATTACCTGAACGTGCAAGTCACCAATACACCTGTGCTGCCGGGCTCCGCA GCTACCGCCACCAGCAAGCTCACCATCATCTTCAAGAACTTCCAAGAGTGTGTGGACCAGAAAGTCTACC AAGCTGAAATGGACGAACTTCCGTCTGCCTTTGCGGACGGCTCCAAAAATGGGGGAGATAAACACGGGGC CAACAGCCTGAAGATCACAGAGAAGGTGTCGGGCCAGCACGTGGAGATCCAGGCCAAGTACATCGGCACC ACCATCGTGGTGCGACAGGTGGGCCGCTACCTGACCTTTGCCGTCCGGATGCCCGAGGAGGTAGTCAACG CCGTGGAGGACCGTGACAGCCAAGGCCTCTACCTCTGCCTGCGGGGCTGCCCGCTCAACCAGCAGATCGA CTTCCAGGCTTTCCGTGCCAACGCTGAAAGCCCTCGCAGGCCAGCAGCCGCCAGTCCCTCTCCCGTGGTC CCCGAGACATTCCCCTATGAGACAGCCGTGGCCAAGTGCAAAGAGAAGCTGCCCGTAGAAGACCTGTACT ACCAGGCCTGTGTCTTCGACCTCCTCACGACTGGTGATGTGAACTTCACGCTGGCTGCCTACTATGCTTT GGAGGATGGCAAGATGCTCCACTCCAACAAGGACAAGCTGCATCTGTTTGAAAGGACTCGGGAGCTGCCA GGAGCCGTGGCCGCCGCCGCCGCCGCTGCCACCACATTCCCCTTGGCCCCCCAGATTCTCCTTGGCACCA TCCCACTTCTGGTCCTCCTGCCTGTGTTGTGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_177740 |
| Insert Size | 1365 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_177740.5, NP_808408.2 |
| RefSeq Size | 3586 bp |
| RefSeq ORF | 1365 bp |
| Locus ID | 244058 |
| UniProt ID | Q6PCX7 |
| Gene Summary | Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG224639 | Rgma (tGFP-tagged) - Mouse RGM domain family member A (Rgma), (10ug) |
CNY 5,040.00 |
|
| MR224639 | Rgma (Myc-DDK-tagged) - Mouse RGM domain family, member A (Rgma) |
CNY 4,560.00 |
|
| MR224639L3 | Lenti ORF clone of Rgma (Myc-DDK-tagged) - Mouse RGM domain family, member A (Rgma) |
CNY 6,460.00 |
|
| MR224639L4 | Lenti ORF clone of Rgma (mGFP-tagged) - Mouse RGM domain family, member A (Rgma) |
CNY 6,460.00 |
