Rgma (NM_177740) Mouse Tagged ORF Clone
CAT#: MG224639
- TrueORF®
Rgma (tGFP-tagged) - Mouse RGM domain family member A (Rgma), (10ug)
ORF Plasmid: DDK
"NM_177740" in other vectors (4)
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CNY 5,040.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | BC059072; C230063O06 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG224639 representing NM_177740
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGCCGCCAAGGGAGAGGCTAGTGGTAACAGGCCGAGCTGGATGGATGGGTATGGGGAGAGGGGCAG GACGTTCAGCCCTGGGATTGTGGCCGACCCTCGCCTTCCTTCTCTGCAGCTTCCCCGCAGCCATCTCCCC CTGCAAAATCCTCAAGTGCAACTCTGAGTTCTGGAGCGCCACGTCATCAGGCAGCCACGCCCCTGCCTCC GACGACGTGCCGGAGTTCTGTGCAGCCCTGCGCACCTACGCCCTGTGCACTCGGCGGACAGCCCGCACCT GCCGGGGCGACCTGGCTTACCACTCGGCTGTCCATGGCATAGAGGACCTTATGAGCCAGCACAACTGCTC CAAGGACGGCCCCACCTCACAGCCGCGAGTGCGCACGCTCCCGCCAGCTGGGGACAGCCAGGAGCGCTCG GATAGCCCCGAGATCTGCCACTATGAGAAGAGTTTCCACAAGCACTCAGCTGCCCCCAACTACACTCACT GCGGCCTCTTTGGGGACCCACACCTCAGGACTTTCACAGACCACTTCCAGACATGCAAGGTGCAAGGCGC CTGGCCTCTCATCGACAATAATTACCTGAACGTGCAAGTCACCAATACACCTGTGCTGCCGGGCTCCGCA GCTACCGCCACCAGCAAGCTCACCATCATCTTCAAGAACTTCCAAGAGTGTGTGGACCAGAAAGTCTACC AAGCTGAAATGGACGAACTTCCGTCTGCCTTTGCGGACGGCTCCAAAAATGGGGGAGATAAACACGGGGC CAACAGCCTGAAGATCACAGAGAAGGTGTCGGGCCAGCACGTGGAGATCCAGGCCAAGTACATCGGCACC ACCATCGTGGTGCGACAGGTGGGCCGCTACCTGACCTTTGCCGTCCGGATGCCCGAGGAGGTAGTCAACG CCGTGGAGGACCGTGACAGCCAAGGCCTCTACCTCTGCCTGCGGGGCTGCCCGCTCAACCAGCAGATCGA CTTCCAGGCTTTCCGTGCCAACGCTGAAAGCCCTCGCAGGCCAGCAGCCGCCAGTCCCTCTCCCGTGGTC CCCGAGACATTCCCCTATGAGACAGCCGTGGCCAAGTGCAAAGAGAAGCTGCCCGTAGAAGACCTGTACT ACCAGGCCTGTGTCTTCGACCTCCTCACGACTGGTGATGTGAACTTCACGCTGGCTGCCTACTATGCTTT GGAGGATGGCAAGATGCTCCACTCCAACAAGGACAAGCTGCATCTGTTTGAAAGGACTCGGGAGCTGCCA GGAGCCGTGGCCGCCGCCGCCGCCGCTGCCACCACATTCCCCTTGGCCCCCCAGATTCTCCTTGGCACCA TCCCACTTCTGGTCCTCCTGCCTGTGTTGTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224639 representing NM_177740
Red=Cloning site Green=Tags(s) MQPPRERLVVTGRAGWMGMGRGAGRSALGLWPTLAFLLCSFPAAISPCKILKCNSEFWSATSSGSHAPAS DDVPEFCAALRTYALCTRRTARTCRGDLAYHSAVHGIEDLMSQHNCSKDGPTSQPRVRTLPPAGDSQERS DSPEICHYEKSFHKHSAAPNYTHCGLFGDPHLRTFTDHFQTCKVQGAWPLIDNNYLNVQVTNTPVLPGSA ATATSKLTIIFKNFQECVDQKVYQAEMDELPSAFADGSKNGGDKHGANSLKITEKVSGQHVEIQAKYIGT TIVVRQVGRYLTFAVRMPEEVVNAVEDRDSQGLYLCLRGCPLNQQIDFQAFRANAESPRRPAAASPSPVV PETFPYETAVAKCKEKLPVEDLYYQACVFDLLTTGDVNFTLAAYYALEDGKMLHSNKDKLHLFERTRELP GAVAAAAAAATTFPLAPQILLGTIPLLVLLPVLW TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_177740 |
ORF Size | 1362 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_177740.5, NP_808408.2 |
RefSeq Size | 3586 bp |
RefSeq ORF | 1365 bp |
Locus ID | 244058 |
UniProt ID | Q6PCX7 |
Gene Summary | Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC216203 | Rgma (untagged) - Mouse RGM domain family, member A (Rgma), (10ug) |
CNY 5,990.00 |
|
MR224639 | Rgma (Myc-DDK-tagged) - Mouse RGM domain family, member A (Rgma) |
CNY 4,560.00 |
|
MR224639L3 | Lenti ORF clone of Rgma (Myc-DDK-tagged) - Mouse RGM domain family, member A (Rgma) |
CNY 6,460.00 |
|
MR224639L4 | Lenti ORF clone of Rgma (mGFP-tagged) - Mouse RGM domain family, member A (Rgma) |
CNY 6,460.00 |