Atg14 (NM_172599) Mouse Untagged Clone

CAT#: MC216889

Atg14 (untagged) - Mouse VATG14 autophagy related 14 homolog (S. cerevisiae) (Atg14), (10ug)



  "NM_172599" in other vectors (4)

CNY 6,370.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 4832427M01; Atg14L; D14Ertd114e; D14Ertd436e
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC216889 representing NM_172599
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTCTCCCAGTGGGAAGGGATCTTGGACGCCCGAGGCTCCTGGTTTTGGGCCGCGGGCGCTAGCAC
GGGACCTGGTGGACTCGGTGGACGACGCCGAGGGCCTTTACGTGGCTGTTGAGCGGTGTCCTCTGTGCAA
CACCACTCGCCGGCGGTTGACTTGCGCCAAGTGCGTCCAGAGCGGTGATTTCGTCTATTTCGACGGCCGC
GACCGGGAGAGGTTTATTGACAAGAAGGAAAGACTAAGCCAACTTAAGAACAAGCAAGAAGAATTTCAGA
AAGAAGTACTAAAAGCTATGGAAGGAAAGCGGCTTACAGATCAGTTGAGATGGAAAATAATGTCATGCAA
GATGAGGATTGAACAGCTGAAGCAAACAATATGTAAAGGAAATGAGGAAATGAAGAAAAATTCTGAAGGT
CTCCTCAAGAACAAGGAAAAGAACCAGAAGCTTTACAGCCGAGCACAGCGGCACCAAGAGAAAAAGGAGA
AGATTCAGCGGCACAACCGCAAGCTTGGGGACCTGGTGGAGAAGAAGACCATTGACTTGAAGAGTCACTA
TGAGCGGTTGGCGCGGCTTCGAAGGTCACACATCCTAGAGCTCACCTCCATCATATTCCCAATCGACGAA
GTGAAGACTTCTGGGAGAGACCCTGCAGACGTGTCTTCAGAGACTGACAGTGCCATGACCTCAAGCATGG
TGAGCAAGCTTGCTGAGGCCCGGAGGACAACTTACCTCTCTGGAAGATGGGTCTGTGATGACCACAATGG
TGACACCAGCATTAGCATCACAGGCCCGTGGATTAGCCTACCAAACAACGGGGACTACTCTGCTTACTAC
AATTGGGTAGAAGAGAAGAAAACAACCCAAGGACCTGACATGGAGCATAACAACCCCGCCTACACTATCA
GCGCCGCGCTGGGCTACGCCACGCAGCTCGTCAACATTGTGTCTCACATACTTGACATCAATCTTCCCAA
AAAGCTGTGCAACAGCGAGTTCTGTGGCGAAAACCTCAGCAAGCAGAAACTGACACGCGCAGTGAGGAAA
CTGAACGCAAACATCCTTTACCTTTGTTCTTCTCAGCATGTAAATCTGGATCAGTTGCAACCACTGCACA
CACTCAGGAACCTGATGCACTTGGTCAGCCCGCGCTCTGAGCACCTAGGCAGGTCAGGACCCTTTGAAGT
TCGAGCAGACCTCGAGGAGTCCATGGAATTTGTGGACCCTGGAGTTGCTGGAGAATCAGACGCGAGTGGA
GATGAGCGTGTAAGCGATGAGGAGACTGACCTGGGCACAGACTGGGAGAACCTGCCAAGCCCCCGATTCT
GTGACATCCCTTCCCAGCCGGTGGAAGTGTCCCAGAGCCAGAGCACCCAGGTGTCCCCACCCATTGCCAG
CAGCAGCGCTGGTGGGATGATCTCCTCCGCTGCGGCCTCGGTGACCTCTTGGTTCAAAGCTTACACTGGA
CACCGCTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_172599
Insert Size 1479 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_172599.4, NP_766187.1
RefSeq Size 3466 bp
RefSeq ORF 1479 bp
Locus ID 100504663
UniProt ID Q8CDJ3
Gene Summary Required for both basal and inducible autophagy (PubMed:19270696, PubMed:19270693). Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1 (By similarity). Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine (PubMed:19270696, PubMed:19270693). Promotes BECN1 translocation from the trans-Golgi network to autophagosomes (By similarity). Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (By similarity). Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation of PIK3C3 by AMPK (PubMed:23332761). Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion (By similarity). Modulates the hepatic lipid metabolism (PubMed:22992773).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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