Cyp27b1 (NM_010009) Mouse Untagged Clone

CAT#: MC217115

Cyp27b1 (untagged) - Mouse cytochrome P450, family 27, subfamily b, polypeptide 1 (Cyp27b1), nuclear gene encoding mitochondrial protein, (10ug)



  "NM_010009" in other vectors (4)

CNY 5,488.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Cp2b; Cyp1; Cyp27b; Cyp40; P450c1; Pddr; Vdd1; Vddr; VddrI; Vdr
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC217115 representing NM_010009
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCCGATTCTCTCAGGTGCATGGCGCTGCGCGGTACGGGCCAGTATGGTCTGGCAGCTTTGGGACAC
TTCGCACAGTTTACGTTGCCGACCCTACACTTGTGGAGCAGCTCCTGCGACAAGAAAGTCACTGTCCAGA
GCGCTGTAGTTTCTCATCATGGGCAGAGCACCGTCGCCGCCACCAGCGTGCTTGCGGATTGCTAACGGCG
GATGGTGAAGAATGGCAGAGGCTCCGAAGTCTTCTGGCCCCGCTCCTCCTCCGGCCACAAGCAGCCGCGG
GCTATGCTGGAACTCTGGACAACGTGGTCCGTGACCTTGTGCGACGACTAAGGCGCCAGCGGGGACGTGG
CTCTGGGCTACCCGGCCTAGTTCTGGACGTGGCAGGAGAGTTTTACAAATTTGGCCTAGAAAGTATAGGC
GCGGTGCTGCTGGGATCGCGCCTGGGCTGCCTAGAGGCTGAAGTCCCTCCTGACACAGAAACCTTCATAC
ATGCAGTGGGCTCAGTGTCTGTGTCTACACTCTTGACCATGGCGATGCCCAACTGGTTGCACCACCTTAT
ACCTGGACCCTGGGCCCGCCTCTGCCGAGACTGGGATCAGATGTTTGCCTTTGCCCAGAGGCACGTGGAG
CTGCGAGAAGGTGAAGCTGCGATGAGGAACCAGGGAAAGCCTGAGGAGGATATGCCGTCTGGGCATCACT
TAACCCACTTCCTTTTTCGGGAAAAGGTGTCTGTCCAGTCCATAGTGGGGAATGTGACAGAGCTACTACT
GGCTGGAGTGGACACGGTATCCAATACGCTCTCCTGGACACTCTATGAGCTTTCCCGGCACCCCGATGTC
CAGACTGCACTCCACTCTGAGATCACAGCTGGGACCCGTGGCTCCTGTGCCCACCCCCATGGCACTGCTC
TGTCCCAGCTGCCCCTGTTAAAGGCTGTGATCAAAGAAGTGTTGAGATTGTACCCTGTGGTACCTGGGAA
TTCCCGTGTCCCAGACAGAGACATCCGTGTAGGAAACTATGTAATTCCCCAAGATACGCTAGTCTCCCTA
TGTCACTATGCCACTTCAAGGGACCCCACACAGTTTCCAGACCCCAACTCTTTTAATCCAGCTCGCTGGC
TGGGGGAGGGTCCGACCCCCCACCCATTTGCATCTCTTCCCTTCGGCTTTGGCAAACGGAGCTGCATCGG
GAGACGCTTGGCAGAGCTTGAGCTACAAATGGCTTTGTCCCAGATCTTGACCCATTTTGAAGTGCTACCT
GAGCCAGGTGCTCTTCCTATCAAGCCCATGACCCGGACTGTCCTGGTCCCTGAGAGGAGCATCAATCTAC
AGTTTGTAGATAGATAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_010009
Insert Size 1347 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC080697, AAH80697
RefSeq Size 2440 bp
RefSeq ORF 1524 bp
Locus ID 13115
UniProt ID O35084
Gene Summary Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D3) to 1-alpha,25-dihydroxyvitamin D3 (1alpha,25(OH)(2)D3), and of 24,25-dihydroxyvitamin D3 (24,25(OH)(2)D3) to 1-alpha,24,25-trihydroxyvitamin D3 (1alpha,24,25(OH)(3)D3). Is also active with 25-hydroxy-24-oxo-vitamin D3. Plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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