Sgo1 (NM_028232) Mouse Untagged Clone

CAT#: MC217322

Sgol1 (untagged) - Mouse shugoshin-like 1 (S. pombe) (Sgol1), (10ug)



  "NM_028232" in other vectors (4)

CNY 6,650.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 3300001M08Rik; C81037; Sgo1; Sgol1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC217322 representing NM_028232
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTAAGGAAAGGTGTCAGAAAAGGTCCTTTCAAGATACCCTTGAAGACATTAAGAATCGAATGAAAG
AAAAAAGGAATAAAAATTTGGCGGGGATTGGGAAACGCAAGTCCTTTATTGTTGCACCGGGCCAAGTACC
CACTAACACTGCTACACTACTGAGATATTACCAAGATAACAACAGGTTGTTAGTCTTGGCTTTGGAAAAT
GAGAAATCCAAAGTGAGAGAAGCACAGGATGTCATCCTGCAACTGAGAAAAGAATGCTACTACCTTACTT
GTCAGCTGTATGCATTGAAAGAGAAGCTAACTTCCCGACAAAGTGAAGAAACTACTCAGAACTGGAAAGG
ACGTCCCTCAGACGTGGTCTCCAGCATTGACAATACGACCAGGGACTTGTCAGGGAAGTCCTTACAGCAA
ATTGCTGTTGAAGAAACTGATTGTCCTTACCAAACCACAGAACCAAGTCCTGCTGTTACTCCAGAGACAC
AGGGTTGCGATTTTGATTCAGGTAAAGTTGAGTCTACTGATGAAGTCTTACCCAGAACTATATCTATCCG
TCGCCATTTAAGGAAAGATTTTAGTAATATAAGCCACTCCACGACTTTGGAGGATTGTAAAGCCAGTCCA
AGAGTGGCACAGTCTCTGGAAGTTAAAGGAAGTAGATGTAGAGAAGTAACCGTAACCCTGCACAGACTTG
AAAATGTTTGTCTGTGGAACAAAGACCAAATTAGCTTATGTTCTAGACTGATTAACCCAGCAAAGATTAC
TGAAACAGAAGTCATTTTATCATCTAAACCTGAACAAATAGAAAGCAAGCATAAACGTGCACGAAAAAGA
AGAGCAGAGCAAAGAAGAACCAAGCAGAGATGCAAATCAAAATCCTCATTGAGGAGTAAGGGGAACAAAA
ACAAAGATAAGCAGGGTTTACCCCCTACTACACTGGATGGAGGTATTGGTTCCTGTGATGCTTACGATTT
TAATCTAAAAGGGACGGTCCACCCCACCCCTTTCCGACAAAAAATGAACAATGGCTGCAACAAAGAAACG
GATAGCAGCAACTCAGAAGTGAGTGACCTCGAATGCAGTACCTCTGAGGATGAGTCTGATGACCTCTACC
TGCCTCCCTCCAAGCGCTTGCGAGACTACAGAGAGTCAGAGAGAGCAGTTACCAGGCCTCGGTCTAAAAG
AGGACTTCAGTACCCAGATGGGAAAGAGAGGAAGGAGGTGCTGCCATCTACAGCTCCTACTGGTATCCCA
CCTGAGACTCAAGAGTCACCTCGTTGTAGCCTAAAGGATGTCACCAATATCCTGCAGTGTCCTAGAGTGA
AGATCAGGAAGCCTTCTCTGCCTCCAAAGCGGCGTGAAGACAGCCCAGCAGTGGCTCTGACTAAACGCAG
GTGTAGCACCATCAAAAGCTATAAAGAGCCAACACTCGCTTCAAAGCTAAGAAGAGGGGACCCTTTCACG
GACTTGTGTTTCTTGAATTCTCCTATTTTCAAGCAGAAAAGGGGTATGAGATGTCCTAAAAGAAGAACCA
AGCAAACACAGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_028232
Insert Size 1554 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_028232.2, NP_082508.1
RefSeq Size 3609 bp
RefSeq ORF 1554 bp
Locus ID 72415
UniProt ID Q9CXH7
Gene Summary Plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Involved in centromeric enrichment of AUKRB in prometaphase.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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