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Lag3 (NM_008479) Mouse Untagged Clone
CAT＃: MC217427
Lag3 (untagged) - Mouse lymphocyte-activation gene 3 (Lag3), (10ug)
CNY 1,200.00
CNY 6,750.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | CD223; LAG-3; Ly66 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC217427 representing NM_008479
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGAGGACCTGCTCCTTGGCTTTTTGCTTCTGGGACTGCTTTGGGAAGCTCCAGTTGTGTCTTCAG GGCCTGGGAAAGAGCTCCCCGTGGTGTGGGCCCAGGAGGGAGCTCCCGTCCATCTTCCCTGCAGCCTCAA ATCCCCCAACCTGGATCCTAACTTTCTACGAAGAGGAGGGGTTATCTGGCAACATCAACCAGACAGTGGC CAACCCACTCCCATCCCGGCCCTTGACCTTCACCAGGGGATGCCCTCGCCTAGACAACCCGCACCCGGTC GCTACACGGTGCTGAGCGTGGCTCCAGGAGGCCTGCGCAGCGGGAGGCAGCCCCTGCATCCCCACGTGCA GCTGGAGGAGCGCGGCCTCCAGCGCGGGGACTTCTCTCTGTGGTTGCGCCCAGCTCTGCGCACCGATGCG GGCGAGTACCACGCCACCGTGCGCCTCCCGAACCGCGCCCTCTCCTGCAGTCTCCGCCTGCGCGTCGGCC AGGCCTCGATGATTGCTAGTCCCTCAGGAGTCCTCAAGCTGTCTGATTGGGTCCTTTTGAACTGCTCCTT CAGCCGTCCTGACCGCCCAGTCTCTGTGCACTGGTTCCAGGGCCAGAACCGAGTGCCTGTCTACAACTCA CCGCGTCATTTTTTAGCTGAAACTTTCCTGTTACTGCCCCAAGTCAGCCCCCTGGACTCTGGGACCTGGG GCTGTGTCCTCACCTACAGAGATGGCTTCAATGTCTCCATCACGTACAACCTCAAGGTTCTGGGTCTGGA GCCCGTAGCCCCTCTGACAGTGTACGCTGCTGAAGGTTCTAGGGTGGAGCTGCCCTGTCATTTGCCCCCA GGAGTGGGGACCCCTTCTTTGCTCATTGCCAAGTGGACTCCTCCTGGAGGAGGTCCTGAGCTCCCCGTGG CTGGAAAGAGTGGCAATTTTACCCTTCACCTTGAGGCTGTGGGTCTGGCACAGGCTGGGACCTACACCTG TAGCATCCATCTGCAGGGACAGCAGCTCAATGCCACTGTCACGTTGGCGGTCATCACAGTGACTCCCAAA TCCTTCGGGTTACCTGGCTCCCGGGGGAAGCTGTTGTGTGAGGTAACCCCGGCATCTGGAAAGGAAAGAT TTGTGTGGCGTCCCCTGAACAATCTGTCCAGGAGTTGCCCGGGCCCTGTGCTGGAGATTCAGGAGGCCAG GCTCCTTGCTGAGCGATGGCAGTGTCAGCTGTACGAGGGCCAGAGGCTTCTTGGAGCGACAGTGTACGCC GCAGAGTCTAGCTCAGGCGCCCACAGTGCTAGGAGAATCTCAGGTGACCTTAAAGGAGGCCATCTCGTTC TCGTTCTCATCCTTGGTGCCCTCTCCCTGTTCCTTTTGGTGGCCGGGGCCTTTGGCTTTCACTGGTGGAG AAAACAGTTGCTACTGAGAAGATTTTCTGCCTTAGAACATGGGATTCAGCCATTTCCGGCTCAGAGGAAG ATAGAGGAGCTGGAGCGAGAACTGGAGACGGAGATGGGACAGGAGCCGGAGCCCGAGCCGGAGCCACAGC TGGAGCCAGAGCCCAGGCAGCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_008479 |
| Insert Size | 3 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC120591, AAI20592 |
| RefSeq Size | 2020 bp |
| RefSeq ORF | 1566 bp |
| Locus ID | 16768 |
| UniProt ID | Q61790 |
| Gene Summary | Lymphocyte activation gene 3 protein: Inhibitory receptor on antigen activated T-cells (PubMed:12209638, PubMed:12421911, PubMed:12672063, PubMed:15100286, PubMed:15634887, PubMed:30580966). Delivers inhibitory signals upon binding to ligands, such as FGL1 (PubMed:30580966). FGL1 constitutes a major ligand of LAG3 and is responsible for LAG3 T-cell inhibitory function (PubMed:30580966). Following TCR engagement, LAG3 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (PubMed:12209638, PubMed:12421911, PubMed:12672063, PubMed:15100286, PubMed:15634887). May inhibit antigen-specific T-cell activation in synergy with PDCD1/PD-1, possibly by acting as a coreceptor for PDCD1/PD-1 (PubMed:21300912). Negatively regulates the proliferation, activation, effector function and homeostasis of both CD8(+) and CD4(+) T-cells (PubMed:12209638, PubMed:12421911, PubMed:12672063, PubMed:15100286, PubMed:15634887). Also mediates immune tolerance: constitutively expressed on a subset of regulatory T-cells (Tregs) and contributes to their suppressive function (PubMed:15485628). Also acts as a negative regulator of plasmacytoid dendritic cell (pDCs) activation (PubMed:19201841). Binds MHC class II (MHC-II); the precise role of MHC-II-binding is however unclear (PubMed:12209638, PubMed:12421911, PubMed:15634887).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG222979 | Lag3 (tGFP-tagged) - Mouse lymphocyte-activation gene 3 (Lag3), (10ug) |
CNY 5,480.00 |
|
| MR222979 | Lag3 (Myc-DDK-tagged) - Mouse lymphocyte-activation gene 3 (Lag3) |
CNY 3,880.00 |
|
| MR222979L3 | Lenti ORF clone of Lag3 (Myc-DDK-tagged) - Mouse lymphocyte-activation gene 3 (Lag3) |
CNY 6,280.00 |
|
| MR222979L4 | Lenti ORF clone of Lag3 (mGFP-tagged) - Mouse lymphocyte-activation gene 3 (Lag3) |
CNY 6,280.00 |
