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Adgrg5 (NM_001033468) Mouse Untagged Clone
CAT＃: MC217540
Gpr114 (untagged) - Mouse G protein-coupled receptor 114 (Gpr114), transcript variant 2, (10ug)
CNY 6,750.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Gm1109; Gpr114; PGR27 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC217540 representing NM_001033468
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCACACGGCGCCCTTTTCTTCTACCTGTGCCTTCTGGCTGCTCAAGTTGTCCTAGTAGAAACCC TGTCAGACCTACTTGTCTTGATGAAGCGCCTTGAGCAGCCCGTAGGCCGGGGCTTGTCTTCCAGAGCGAG GCATATCCACAGCCTGGAGCAGAAGCTGCTCAATGCCAGCTTCGGCGGGCACAACTTGACCTTGCAGACA AACTCCATCCAGTCTCTTGTCTTCAAGCTGAGCTGCGACTTTCCTGGTCTTTCCCTGTCCAGCACCACAC TGACAAATGTCTCCCAGGTGCGAGCCCCACATGCCATGCAATTCCCTGCTGAGCTGACCAAGGGTGCCTG TGTGACCTCCCGGCCTGCTGAGCTTCGACTCATCTGCATCTATTTCTTCACTGCACACCTCTTTCAGGAT GACCGGAACTCATCACTGCTCAATAACTATGTCCTGGGGGCCCAGCTGGATCACAGGCCTGTGAACAACC TTCAGAAGCCAGTCAACATCAGCTTCTGGCATAATCGGAGTCTGGAAGGGTATACAGTATCCTGTGTTTT CTGGAAGGAGGGAGCCAGCAAGAGCAGCTGGGGGGCCTGGAGCCCTGAGGGCTGTTATACAGAGCAGCCC TCAGCCACCCAGGTTCTCTGCCATTGCAACCATCTCACCTACTTCGCTGTGCTTATGCTCTCTGGAGACC CCGTGCCCGCTGAGCTGCAGGTGCCTCTTGAGTACATCTCCTTTGTGGGTTGCAGCATCTCCATCGTGGC CTCGCTGCTCACCATACTGCTGTACGCTCAGTCCAGGAAGCAAAGCGATTCCACCACACGTATCCACATG AACCTGAATGGCTCTGTTCTGCTCCTGAACGTCACCTTCCTTCTGAGCTCCCAGATGACCCTGCCCACCA TGCCCAGGCCCGTCTGCAAGGTGCTGGCTGCTGTCCTACACTACGCACTGCTCAGCAGCCTTACCTGGAT GGCCATCGAAGGCTTCAACCTCTACCTTTTCCTGGGGCGTGTCTACAACGCCTACATTCGCCGATACTTG CTCAAGCTCTGCATGCTGGGCTGGGGGTTTCCAGCCCTCTTGGTGCTGCTGCTTCTGATGATCAAGAGCT CAGTGTATGGACCCTGTGTGACCTCACTCTCCAAAAGCCAGGAAAATGGCACAGGCTTCCAGAATGTGTC CATGTGCTGGATCCGAAGTCCCATGGTACACAGCATCCTGGTCATGGGCTATGGTGGCTTCACATCTCTG TTCAACCTGGTGGTGCTGGCCTGGGCTCTGTGGATCTTGTGCAGGCTTCGGGCACGGGAGAAGGCACTGA GTCCCTGGGCCTACCGGGACACTGCCATGGTGTTGGGTCTCACTGTGCTGCTTGGCACCACCTGGACCCT AGCCTTCTTCTCCTTCGGTGTGTTCCTGCTGCCTCAGCTCTTCCTCTTTACCATCTTCAACTCGCTCTAT GGTTTCTTCCTCTTCCTGTGGTTCTGCTCACAAAAGCGTTACTCGGATGCCGAAGCCAAGGCGGAGATGG AGGCAGTCAGCTCCTCCCAGATGACACACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001033468 |
| Insert Size | 1572 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001033468.3, NP_001028640.2 |
| RefSeq Size | 2812 bp |
| RefSeq ORF | 1572 bp |
| Locus ID | 382045 |
| UniProt ID | Q3V3Z3 |
| Gene Summary | Adhesion G protein-coupled receptor (GPCR). Transduces intracellular signals through coupling to guanine nucleotide-binding protein G(s) subunit alpha and activation of adenylate cyclase pathway (PubMed:22575658). Isoform 1, but not isoform 2, is constitutively active, as evidenced by elevated basal cAMP levels, and responds to mechanical activation (shaking) (PubMed:26499266).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) has two alternate splice sites in the 5' UTR and CDS respectively, as compared to variant 1. The resulting isoform (2) lacks an internal amino acid, as compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG216358 | Gpr114 (tGFP-tagged) - Mouse G protein-coupled receptor 114 (Gpr114) transcript variant 2, (10ug) |
CNY 5,040.00 |
|
| MR216358 | Gpr114 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 114 (Gpr114), transcript variant 2 |
CNY 3,912.00 |
|
| MR216358L3 | Lenti ORF clone of Gpr114 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 114 (Gpr114), transcript variant 2 |
CNY 6,460.00 |
|
| MR216358L4 | Lenti ORF clone of Gpr114 (mGFP-tagged) - Mouse G protein-coupled receptor 114 (Gpr114), transcript variant 2 |
CNY 6,460.00 |
