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Cerk (NM_145475) Mouse Untagged Clone
CAT＃: MC217643
Cerk (untagged) - Mouse ceramide kinase (Cerk), (10ug)
CNY 6,840.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI848610; D330016D08Rik; mKIAA1646 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC217643 representing NM_145475
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGCAATGGGGGCGGCGGAGCCGCTGCACTCCGTGCTGTGGGTGAAACGGCGACGCTGTGCCGTCA GCCTGGAGCCCGCGCGGGCTCTGCTACGCTGGTGGCGGAGCCCGGAGCCCGGGCCCTCCGCGCCGGGTGC CGATGCCCGCTCGGTACTGGTGTCGGAGATCATCGCTGTTGAGGAAAAAGACGACTGCGAGAAACACGCC TCCAGTGGCCGATGGCATAAAATGGAAAATCCGTTCGCATTCACAGTCCACCGTGTGAAGCGAGTACGAC ATCACCGCTGGAAGTGGGCACGGGTGACCTTCTGGAGCGCCGACGAGCAGCTGTGTCACCTGTGGCTGCA GACCCTCCGTGGGCTGCTGGAGAGCCTGACTTCAAGACCGAAGCACTTGCTGGTATTCATCAACCCTTTC GGAGGGAAAGGTCAGGGCAAGCGCATCTATGAAAAAACAGTGGCGCCTCTGTTTACCTTGGCTTCCATCA CTACGGAGATCATCATTACAGAGCATGCCAACCAAGCCAAGGAGACTTTATACGAGATCAACACAGACAG CTATGATGGCATCGTGTGCGTAGGTGGGGACGGCATGTTCAGCGAGGTGCTGCATGGGGTGATTGGGAGG ACGCAGCAGAGCGCTGGTATCGACCCCAATCACCCCCGAGCCGTGCTGGTGCCCAGTACCCTCAGGATCG GCATCATACCCGCAGGGTCCACAGATTGTGTGTGTTACTCAACAGTGGGCACAAACGACGCAGAGACATC GGCTTTGCACATCATTATTGGGGACTCACTGGCAATAGACGTGTCCTCTGTGCACTACCATAACACGCTG CTGCGGTACTCGGTTTCTCTGCTGGGCTACGGTTTCTACGGGGACTTAATCAAGGACAGTGAAAAGAAAC GGTGGATGGGCCTCGTCCGGTATGATTTCTCAGGGTTGAAGACCTTTCTCTCTCATCAGTACTATGAAGG GACACTGTCCTTCCTCCCAGCACAGCACACGGTGGGATCTCCACGGGACAATAAACCCTGCCGGGCTGGG TGCTTCGTGTGCAGGCAGAGCAAGCAACAGCTGGAAGAAGAAGAGAAGAAAGCCCTGTATGGCCTGGAGA ACGCCGAGGAAATGGAAGAGTGGCAAGTGACATGTGGGAAGTTCCTGGCCATCAATGCCACCAACATGTC CTGTGCTTGTCCTCGGAGCCCTGGGGGCCTGTCCCCATTTGCCCATCTGGGAGATGGGTCTTCTGACCTC ATCCTTATCCGGAAGTGCTCCAGGTTCAACTTCCTGAGATTCCTCATCCGGCACACGAACCAGGAGGACC AGTTCGACTTCACTTTCGTTGAAGTTTATCGAGTCAAGAAATTCCACTTCACGTCGAAGCACGTGGAAGA CGAGGACAATGACTCGAAGGAACAAGAGAAGCAGAAGTTTGGGAAGATCTGCAAGGACAGACCCTCTTGC ACTTGCTCAGCCTCCAGAAGCTCCTGGAACTGCGATGGCGAAGTCATGCACAGCCCGGCCATTGAGGTCA GGGTCCACTGCCAGCTGGTGCGCCTCTTTGCTCGGGGAATCGAGGAAGAGTCATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_145475 |
| Insert Size | 1596 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_145475.4, NP_663450.3 |
| RefSeq Size | 4562 bp |
| RefSeq ORF | 1596 bp |
| Locus ID | 223753 |
| UniProt ID | Q8K4Q7 |
| Gene Summary | Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate (PubMed:16269826). Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines (PubMed:16269826). Shows a greater preference for D-erythro isomer of ceramides (By similarity). Binds phosphoinositides (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG218633 | Cerk (tGFP-tagged) - Mouse ceramide kinase (Cerk), (10ug) |
CNY 5,130.00 |
|
| MR218633 | Cerk (Myc-DDK-tagged) - Mouse ceramide kinase (Cerk) |
CNY 3,952.00 |
|
| MR218633L3 | Lenti ORF clone of Cerk (Myc-DDK-tagged) - Mouse ceramide kinase (Cerk) |
CNY 6,560.00 |
|
| MR218633L4 | Lenti ORF clone of Cerk (mGFP-tagged) - Mouse ceramide kinase (Cerk) |
CNY 6,560.00 |
