Pftk1 (BC068134) Mouse Untagged Clone
CAT#: MC217730
Pftk1 (untagged) - Mouse PFTAIRE protein kinase 1 (cDNA clone MGC:95907 IMAGE:6834018), (10ug)
CNY 6,940.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | mKIAA0834; Pftk1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>BC068134
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTACCCGGAACTGCCAGGGGACAGATTCAGTGATCAAGCACCTGGACACAATTCCTGAAGACAAGA AAGTCAGGGTTCAGAGGACGCAGAGCACTTTTGACCCATTTGAGAAACCAGCCAACCAAGTCAAAAGGGT CCATTCTGAGAACAATGCATGCATTAACTTTAAATCCTCCTCTGCTGGCAAAGAGTCACCTAAAGTTCGG CGGCACTCCAGCCCCAGCTCGCCAACGAGTCCCAAATTTGGAAAAGCTGACTCATACGAAAAACTGGAAA AACTGGGGGAAGGATCTTATGCAACAGTGTACAAAGGGAAAAGCAAAGTGAATGGGAAGCTGGTGGCTCT AAAGGTGATCCGGCTGCAGGAAGAAGAGGGCACACCTTTCACAGCCATCAGGGAAGCTTCCCTGTTGAAA GGACTAAAGCACGCCAACATCGTGTTGCTTCACGACATCATCCACACTAAGGAAACCCTGACCCTTGTCT TTGAATACGTGCACACTGATTTATGTCAGTACATGGACAAGCACCCTGGAGGACTCCATCCAGATAATGT GAAGTTGTTTTTATTTCAGCTGCTGCGAGGACTGTCTTACATCCACCAGCGTTATATTTTGCACAGAGAC CTGAAACCGCAGAACCTTCTCATCAGCGATACGGGGGAGTTGAAGCTGGCAGATTTCGGTCTGGCAAGAG CAAAATCCGTCCCTAGCCACACATACTCCAATGAAGTGGTTACCTTGTGGTACAGACCTCCAGATGTTCT TCTGGGCTCTACAGAATATTCCACCTGCCTTGACATGTGGGGAGTTGGCTGTATCTTCGTTGAGATGATC CAAGGAGTTGCTGCGTTTCCAGGAATGAAAGACATTCAGGATCAACTTGAACGGATATTTCTGGTTCTTG GAACACCGAATGAGGACACGTGGCCTGGAGTTCATTCTTTACCACATTTTAAGCCAGAACGCTTTACCGT GTACAGCTCTAAAAGCCTTAGACAAGCATGGAATAAGCTCAGCTATGTAAATCATGCTGAAGACTTGGCC TCCAAGCTTCTCCAGTGTTCCCCAAAGAACAGGCTATCAGCACAGGCCGCCTTGAGCCATGAGTATTTCA GCGATCTGCCTCCACGGCTATGGGAGCTGACTGATATGTCTTCTATTTTTACCGTCCCAAATGTGAGATT GCAACCAGAAGCTGGAGAGAGCATGAGGGCCTTTGGAAAAAACAATAGTTATGGGAAAAGCCTATCGAAC AGCAAACACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | BC068134 |
Insert Size | 1272 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC068134, AAH68134 |
RefSeq Size | 5127 bp |
RefSeq ORF | 1271 bp |
Locus ID | 18647 |
Gene Summary | Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-1490', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG206736 | Pftk1 (tGFP-tagged) - Mouse PFTAIRE protein kinase 1 (cDNA clone MGC:95907 IMAGE:6834018) |
CNY 2,660.00 |
|
MR206736 | Pftk1 (Myc-DDK-tagged) - Mouse PFTAIRE protein kinase 1 (cDNA clone MGC:95907 IMAGE:6834018) |
CNY 3,656.00 |
|
MR206736L3 | Lenti ORF clone of Pftk1 (Myc-DDK-tagged) - Mouse PFTAIRE protein kinase 1 (cDNA clone MGC:95907 IMAGE:6834018) |
CNY 4,280.00 |