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Pftk1 (BC068134) Mouse Tagged ORF Clone
CAT＃: MG206736
- TrueORF®
Pftk1 (tGFP-tagged) - Mouse PFTAIRE protein kinase 1 (cDNA clone MGC:95907 IMAGE:6834018)
ORF Plasmid: DDK
"BC068134" in other vectors (3)
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CNY 2,660.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | mKIAA0834; Pftk1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG206736 representing BC068134
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTACCCGGAACTGCCAGGGGACAGATTCAGTGATCAAGCACCTGGACACAATTCCTGAAGACAAGA AAGTCAGGGTTCAGAGGACGCAGAGCACTTTTGACCCATTTGAGAAACCAGCCAACCAAGTCAAAAGGGT CCATTCTGAGAACAATGCATGCATTAACTTTAAATCCTCCTCTGCTGGCAAAGAGTCACCTAAAGTTCGG CGGCACTCCAGCCCCAGCTCGCCAACGAGTCCCAAATTTGGAAAAGCTGACTCATACGAAAAACTGGAAA AACTGGGGGAAGGATCTTATGCAACAGTGTACAAAGGGAAAAGCAAAGTGAATGGGAAGCTGGTGGCTCT AAAGGTGATCCGGCTGCAGGAAGAAGAGGGCACACCTTTCACAGCCATCAGGGAAGCTTCCCTGTTGAAA GGACTAAAGCACGCCAACATCGTGTTGCTTCACGACATCATCCACACTAAGGAAACCCTGACCCTTGTCT TTGAATACGTGCACACTGATTTATGTCAGTACATGGACAAGCACCCTGGAGGACTCCATCCAGATAATGT GAAGTTGTTTTTATTTCAGCTGCTGCGAGGACTGTCTTACATCCACCAGCGTTATATTTTGCACAGAGAC CTGAAACCGCAGAACCTTCTCATCAGCGATACGGGGGAGTTGAAGCTGGCAGATTTCGGTCTGGCAAGAG CAAAATCCGTCCCTAGCCACACATACTCCAATGAAGTGGTTACCTTGTGGTACAGACCTCCAGATGTTCT TCTGGGCTCTACAGAATATTCCACCTGCCTTGACATGTGGGGAGTTGGCTGTATCTTCGTTGAGATGATC CAAGGAGTTGCTGCGTTTCCAGGAATGAAAGACATTCAGGATCAACTTGAACGGATATTTCTGGTTCTTG GAACACCGAATGAGGACACGTGGCCTGGAGTTCATTCTTTACCACATTTTAAGCCAGAACGCTTTACCGT GTACAGCTCTAAAAGCCTTAGACAAGCATGGAATAAGCTCAGCTATGTAAATCATGCTGAAGACTTGGCC TCCAAGCTTCTCCAGTGTTCCCCAAAGAACAGGCTATCAGCACAGGCCGCCTTGAGCCATGAGTATTTCA GCGATCTGCCTCCACGGCTATGGGAGCTGACTGATATGTCTTCTATTTTTACCGTCCCAAATGTGAGATT GCAACCAGAAGCTGGAGAGAGCATGAGGGCCTTTGGAAAAAACAATAGTTATGGGAAAAGCCTATCGAAC AGCAAACAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG206736 representing BC068134
Red=Cloning site Green=Tags(s) MSTRNCQGTDSVIKHLDTIPEDKKVRVQRTQSTFDPFEKPANQVKRVHSENNACINFKSSSAGKESPKVR RHSSPSSPTSPKFGKADSYEKLEKLGEGSYATVYKGKSKVNGKLVALKVIRLQEEEGTPFTAIREASLLK GLKHANIVLLHDIIHTKETLTLVFEYVHTDLCQYMDKHPGGLHPDNVKLFLFQLLRGLSYIHQRYILHRD LKPQNLLISDTGELKLADFGLARAKSVPSHTYSNEVVTLWYRPPDVLLGSTEYSTCLDMWGVGCIFVEMI QGVAAFPGMKDIQDQLERIFLVLGTPNEDTWPGVHSLPHFKPERFTVYSSKSLRQAWNKLSYVNHAEDLA SKLLQCSPKNRLSAQAALSHEYFSDLPPRLWELTDMSSIFTVPNVRLQPEAGESMRAFGKNNSYGKSLSN SKH TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC068134 |
| ORF Size | 1269 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC068134, AAH68134 |
| RefSeq Size | 5127 bp |
| RefSeq ORF | 1271 bp |
| Locus ID | 18647 |
| Gene Summary | Serine/threonine-protein kinase involved in the control of the eukaryotic cell cycle, whose activity is controlled by an associated cyclin. Acts as a cell-cycle regulator of Wnt signaling pathway during G2/M phase by mediating the phosphorylation of LRP6 at 'Ser-1490', leading to the activation of the Wnt signaling pathway. Acts as a regulator of cell cycle progression and cell proliferation via its interaction with CCDN3. Phosphorylates RB1 in vitro, however the relevance of such result remains to be confirmed in vivo. May also play a role in meiosis, neuron differentiation and may indirectly act as a negative regulator of insulin-responsive glucose transport (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217730 | Pftk1 (untagged) - Mouse PFTAIRE protein kinase 1 (cDNA clone MGC:95907 IMAGE:6834018), (10ug) |
CNY 6,940.00 |
|
| MR206736 | Pftk1 (Myc-DDK-tagged) - Mouse PFTAIRE protein kinase 1 (cDNA clone MGC:95907 IMAGE:6834018) |
CNY 3,656.00 |
|
| MR206736L3 | Lenti ORF clone of Pftk1 (Myc-DDK-tagged) - Mouse PFTAIRE protein kinase 1 (cDNA clone MGC:95907 IMAGE:6834018) |
CNY 4,280.00 |
