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Ppp4r2 (BC056340) Mouse Untagged Clone
CAT＃: MC218020
Ppp4r2 (untagged) - Mouse protein phosphatase 4, regulatory subunit 2 (cDNA clone MGC:73463 IMAGE:5703151), (10ug)
CNY 6,940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | BE691708; C230060M08Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC056340
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGTCGAGAGGCTGCAGGAGGCGCTGAAAGATTTTGAGAAGAGAGGGAAAAAGGAAGTTTGTCCTG TACTGGATCAGTTTCTCTGTCATGTAGCTAAAACTGGAGAAACAATGATTCAGTGGTCCCAATTTAAAGG CTATTTCATTTTCAAACTGGAGAAAGTGATGGATGATTTCAGAACTTCAGCTCCTGAACCAAGAGGTCCT CCCAATCCTAATGTTGAATATATTCCCTTTGATGAAATGAAGGAAAGAATACTGAAAATTGTCACTGGAT TTAATGGTATCCCTTTTACTATTCAGCGGCTATGTGAATTGCTAACGGATCCGAGAAGAAACTATACAGG AACAGACAAGTTTCTCAGAGGAGTAGAGAAGAATGTGATGGTTGTTAGCTGCGTTTGTCCATCCTCAGAG AAGAACAATTCTAATAGCTTAAATAGAATGAATGGTGTTATGTTTCCTGGAAACTCACCAAACTATACTG ACAGGTCTAATATAAACGGGCCTGGAACACCTAGGCCACTTAATCGACCAAAGCTTTCTTTGTCAGCCCC CTTGACAACAAATGGTTTGCCTGAGAGCACAGATAGCAAAGATTCTGAGCTGCAGCTAAGTGAAGAGAAA GGCCACAGTGATTCTTCAGCCTCTGAATCAGAAGTTTCCTTACTGAGCCCTGTTAAAAATAAACATCCAG ATGAAGATGCTGTGGAGTCTGAGGAACATGAGGTGAAAAGACTGAAGTTTGACAAAGAAGGTGACGTCAG AGAGACAGCTAGCCAAACGGTGTCTGGTGAAGTCTCTTCAGTTAGAGCAGAGGAAACGGAAACAGCAGCT CCCCCTCCTGACAAGGACAGAGAAAGTCGAACCAGGCAGCACTGTACAGAAGAGGAGGAGGAGGAGGAGG AAGAAGAGGAAGAGGAGGAGGAAGAATCGTTTATGACACCAAGAGAAATGGTCCCAGAAAGAAAAAATCA AGAAAAGGAATCTGATGACGCCTTAACTGTGAATGAAGAGACTTCAGAGGAGAGCCATCAGATGGAAGGC TCTGGTGTGTCTCCAGCTCAGACAGACTCCACTTCAGAAAGGAGTGACAGTGCAGGGGCCTCAAGGAGTG GCTCTGACTGCCTGGAGACACAGGAGTCAGGAGGGCCCCCTTCCAGTAAGACTGGAGAGAGTGTGTCAGT GCCGTCGTCCATGGAGAGTGAGGAAGCCGCAGAAGTCACAGATGACCCAATGGAGCAAGACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | BC056340 |
| Insert Size | 1254 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC056340, AAH56340 |
| RefSeq Size | 2769 bp |
| RefSeq ORF | 1253 bp |
| Locus ID | 232314 |
| Gene Summary | Regulatory subunit of serine/threonine-protein phosphatase 4 (PP4). May regulate the activity of PPP4C at centrosomal microtubule organizing centers. Its interaction with the SMN complex leads to enhance the temporal localization of snRNPs, suggesting a role of PPP4C in maturation of spliceosomal snRNPs. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on 'Ser-140' (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair (By similarity). Mediates RPA2 dephosphorylation by recruiting PPP4C to RPA2 in a DNA damage-dependent manner. RPA2 dephosphorylation is required for the efficient RPA2-mediated recruitment of RAD51 to chromatin following double strand breaks, an essential step for DNA repair (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG206596 | Ppp4r2 (tGFP-tagged) - Mouse protein phosphatase 4, regulatory subunit 2 (cDNA clone MGC:73463 IMAGE:5703151) |
CNY 2,660.00 |
|
| MR206596 | Ppp4r2 (Myc-DDK-tagged) - Mouse protein phosphatase 4, regulatory subunit 2 (cDNA clone MGC:73463 IMAGE:5703151) |
CNY 3,656.00 |
|
| MR206596L3 | Lenti ORF clone of Ppp4r2 (Myc-DDK-tagged) - Mouse protein phosphatase 4, regulatory subunit 2 (cDNA clone MGC:73463 IMAGE:5703151) |
CNY 4,280.00 |
|
| MR206596L4 | Lenti ORF clone of Ppp4r2 (mGFP-tagged) - Mouse protein phosphatase 4, regulatory subunit 2 (cDNA clone MGC:73463 IMAGE:5703151) |
CNY 4,280.00 |
