Dusp7 (BC010207) Mouse Untagged Clone

CAT#: MC218030

Dusp7 (untagged) - Mouse dual specificity phosphatase 7 (cDNA clone MGC:11703 IMAGE:3964527), (10ug)



  "BC010207" in other vectors (4)

CNY 6,940.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms MKPX, MKP-X, PYST2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC010207
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCGCCGCCTGCGCAAAGGCAACCTGCCCATCCGCTCCATCATTCCCAACCATGCCGACAAGGAGC
GCTTCGCCACGCGCTGCAAGGCGGCCACCGTGCTGCTCTACGACGAGGCCACGGCGGAGTGGCAGCCCGA
GCCCGGCGCCCCCGCCTCCGTTCTCGGCCTGCTCCTGCAGAAGCTGCGAGACGACGGCTGCCAGGCCTAC
TACCTCCAAGGTGGTTTCAACAAGTTCCAGACGGAATACTCTGAGCATTGTGAGACTAACGTGGACAGCT
CGTCCTCCCCGAGTGGCTCGCCACCCACCTCCGTGCTGGGCCTGGGGGGCCTGCGCATCAGCTCTGACTG
CTCAGACGGCGAGTCGGACCGAGAGCTGCCCAGCAGTGCCACTGAGTCGGATGGCAGCCCTGTACCATCC
AGCCAGCCGGCCTTTCCGGTCCAGATCCTGCCCTACCTCTACCTCGGCTGTGCCAAGGACTCTACCAACC
TGGATGTACTTGGCAAATACGGCATCAAATACATCCTCAACGTCACACCCAACCTGCCCAACGCCTTTGA
GCACGGTGGTGAGTTCACCTACAAGCAAATCCCCATCTCTGACCACTGGAGCCAGAACCTCTCTCAGTTC
TTCCCCGAGGCCATCAGCTTCATTGATGAAGCCCGCTCCAAGAAGTGTGGTGTCTTGGTGCACTGCCTAG
CAGGCATCAGCCGTTCAGTGACAGTTACTGTAGCCTACCTGATGCAGAAGATGAACCTGTCCCTCAATGA
TGCCTATGACTTCGTCAAGAGGAAAAAGTCTAACATCTCGCCCAACTTCAACTTCATGGGGCAGCTGCTG
GACTTTGAGCGGACGCTGGGGCTCAGCAGCCCATGCGACAACCACGCACCCAGTGAGCAGCTCTACTTCT
CCACGCCCACCAACCATAACCTGTTCCCGATCAACACGCTCGAGTCCACGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Restriction Sites SgfI-MluI     
ACCN BC010207
Insert Size 963 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC010207, AAH10207
RefSeq Size 2584 bp
RefSeq ORF 962 bp
Locus ID 235584
Gene Summary Dual specificity protein phosphatase (PubMed:27783954). Shows high activity towards MAPK1/ERK2 (By similarity). Also has lower activity towards MAPK14 and MAPK8 (By similarity). In arrested oocytes, plays a role in meiotic resumption (PubMed:27783954). Promotes nuclear envelope breakdown and activation of the CDK1/Cyclin-B complex in oocytes, probably by dephosphorylating and inactivating the conventional protein kinase C (cPKC) isozyme PRKCB (PubMed:27783954). May also inactivate PRKCA and/or PRKCG (PubMed:27783954). Also important in oocytes for normal chromosome alignment on the metaphase plate and progression to anaphase, where it might regulate activity of the spindle-assembly checkpoint (SAC) complex (PubMed:27783954).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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