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Dusp7 (BC010207) Mouse Tagged ORF Clone
CAT＃: MG204628
- TrueORF®
Dusp7 (tGFP-tagged) - Mouse dual specificity phosphatase 7 (cDNA clone MGC:11703 IMAGE:3964527)
ORF Plasmid: DDK
"BC010207" in other vectors (4)
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CNY 2,850.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | MKPX, MKP-X, PYST2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG204628 representing BC010207
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCGCCGCCTGCGCAAAGGCAACCTGCCCATCCGCTCCATCATTCCCAACCATGCCGACAAGGAGC GCTTCGCCACGCGCTGCAAGGCGGCCACCGTGCTGCTCTACGACGAGGCCACGGCGGAGTGGCAGCCCGA GCCCGGCGCCCCCGCCTCCGTTCTCGGCCTGCTCCTGCAGAAGCTGCGAGACGACGGCTGCCAGGCCTAC TACCTCCAAGGTGGTTTCAACAAGTTCCAGACGGAATACTCTGAGCATTGTGAGACTAACGTGGACAGCT CGTCCTCCCCGAGTGGCTCGCCACCCACCTCCGTGCTGGGCCTGGGGGGCCTGCGCATCAGCTCTGACTG CTCAGACGGCGAGTCGGACCGAGAGCTGCCCAGCAGTGCCACTGAGTCGGATGGCAGCCCTGTACCATCC AGCCAGCCGGCCTTTCCGGTCCAGATCCTGCCCTACCTCTACCTCGGCTGTGCCAAGGACTCTACCAACC TGGATGTACTTGGCAAATACGGCATCAAATACATCCTCAACGTCACACCCAACCTGCCCAACGCCTTTGA GCACGGTGGTGAGTTCACCTACAAGCAAATCCCCATCTCTGACCACTGGAGCCAGAACCTCTCTCAGTTC TTCCCCGAGGCCATCAGCTTCATTGATGAAGCCCGCTCCAAGAAGTGTGGTGTCTTGGTGCACTGCCTAG CAGGCATCAGCCGTTCAGTGACAGTTACTGTAGCCTACCTGATGCAGAAGATGAACCTGTCCCTCAATGA TGCCTATGACTTCGTCAAGAGGAAAAAGTCTAACATCTCGCCCAACTTCAACTTCATGGGGCAGCTGCTG GACTTTGAGCGGACGCTGGGGCTCAGCAGCCCATGCGACAACCACGCACCCAGTGAGCAGCTCTACTTCT CCACGCCCACCAACCATAACCTGTTCCCGATCAACACGCTCGAGTCCACG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG204628 representing BC010207
Red=Cloning site Green=Tags(s) MLRRLRKGNLPIRSIIPNHADKERFATRCKAATVLLYDEATAEWQPEPGAPASVLGLLLQKLRDDGCQAY YLQGGFNKFQTEYSEHCETNVDSSSSPSGSPPTSVLGLGGLRISSDCSDGESDRELPSSATESDGSPVPS SQPAFPVQILPYLYLGCAKDSTNLDVLGKYGIKYILNVTPNLPNAFEHGGEFTYKQIPISDHWSQNLSQF FPEAISFIDEARSKKCGVLVHCLAGISRSVTVTVAYLMQKMNLSLNDAYDFVKRKKSNISPNFNFMGQLL DFERTLGLSSPCDNHAPSEQLYFSTPTNHNLFPINTLEST TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC010207 |
| ORF Size | 962 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC010207, AAH10207 |
| RefSeq Size | 2584 bp |
| RefSeq ORF | 962 bp |
| Locus ID | 235584 |
| Gene Summary | Dual specificity protein phosphatase (PubMed:27783954). Shows high activity towards MAPK1/ERK2 (By similarity). Also has lower activity towards MAPK14 and MAPK8 (By similarity). In arrested oocytes, plays a role in meiotic resumption (PubMed:27783954). Promotes nuclear envelope breakdown and activation of the CDK1/Cyclin-B complex in oocytes, probably by dephosphorylating and inactivating the conventional protein kinase C (cPKC) isozyme PRKCB (PubMed:27783954). May also inactivate PRKCA and/or PRKCG (PubMed:27783954). Also important in oocytes for normal chromosome alignment on the metaphase plate and progression to anaphase, where it might regulate activity of the spindle-assembly checkpoint (SAC) complex (PubMed:27783954).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218030 | Dusp7 (untagged) - Mouse dual specificity phosphatase 7 (cDNA clone MGC:11703 IMAGE:3964527), (10ug) |
CNY 6,940.00 |
|
| MR204628 | Dusp7 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 7 (cDNA clone MGC:11703 IMAGE:3964527) |
CNY 2,400.00 |
|
| MR204628L3 | Lenti ORF clone of Dusp7 (Myc-DDK-tagged) - Mouse dual specificity phosphatase 7 (cDNA clone MGC:11703 IMAGE:3964527) |
CNY 4,750.00 |
|
| MR204628L4 | Lenti ORF clone of Dusp7 (mGFP-tagged) - Mouse dual specificity phosphatase 7 (cDNA clone MGC:11703 IMAGE:3964527) |
CNY 4,750.00 |
