United States
Germany
Japan
United Kingdom
China
Tyms (BC020139) Mouse Untagged Clone
CAT＃: MC218262
Tyms (untagged) - Mouse thymidylate synthase (cDNA clone MGC:28246 IMAGE:3994204), (10ug)
CNY 6,940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | TS |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>BC020139
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGTGGTTGGCTCCGAGCTGCAGTCCGATGCTCAGCAGCTGAGCGCGGAAGCCCCGCAGCATGGAG AACTCCAGTACCTGAGGCAGGTGGAACACATTTTGCGCTGCGGCTTCAAGAAGGAGGACCGCACGGGCAC AGGCACCCTGTCGGTGTTCGGCATGCAGGCACGATACAGCCTGAGAGATGAATTTCCTCTGCTCACAACC AAACGAGTGTTCTGGAAGGGTGTTTTGGAGGAGTTGTTGTGGTTTATCAAGGGATCCACAAATGCTAAAG AATTGTCCTCCAAGGGAGTGAGAATCTGGGATGCCAATGGATCCCGAGATTTTCTGGACAGCTTGGGATT TTCTGCCCGACAGGAAGGGGACCTGGGCCCAGTTTATGGTTTCCAATGGAGGCATTTTGGAGCAGAGTAC AAAGATATGGATTCAGATTACTCGGGACAAGGAGTAGACCAGCTGCAAAAAGTGATTGACACCATCAAAA CCAACCCTGATGACAGAAGAATCATCATGTGTGCCTGGAACCCAAAAGATCTTCCCCTGATGGCACTGCC TCCTTGCCATGCCCTCTGTCAGTTCTATGTGGTGAATGGGGAACTGTCTTGCCAGCTTTACCAGAGGTCA GGAGATATGGGTCTGGGCGTGCCCTTCAACATTGCCAGCTATGCTCTGCTCACCTACATGATTGCACATA TCACAGGCCTGCAGCCAGGTGATTTTGTCCACACTTTGGGAGATGCACATATTTACCTGAATCATATAGA GCCGCTGAAAATTCAGCTACAGCGAGAACCAAGACCTTTCCCAAAGCTCAAAATCCTTCGAAAAGTTGAG ACAATCGATGATTTCAAAGTTGAAGACTTTCAGATTGAAGGGTATAATCCACATCCAACGATTAAAATGG AAATGGCTGTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | BC020139 |
| Insert Size | 924 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC020139, AAH20139 |
| RefSeq Size | 986 bp |
| RefSeq ORF | 923 bp |
| Locus ID | 22171 |
| Gene Summary | This gene encodes an enzyme that catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate as a cofactor. This function maintains the thymidine-5-prime monophosphate concentration critical for DNA replication and repair. The encoded enzyme is a target for cancer chemotherapeutic agents. The majority of transcripts for this gene lack a 3' UTR (PMID: 3022294, 3444407). The stop codon in these transcripts is UAA, compared to the UAG found in the genome and longer transcripts, as the polyA site is located within the stop codon (PMID: 3444407, 2157203). A related pseudogene has been identified on chromosome 10. [provided by RefSeq, Mar 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG204343 | Tyms (tGFP-tagged) - Mouse thymidylate synthase (cDNA clone MGC:28246 IMAGE:3994204) |
CNY 2,850.00 |
|
| MR204343 | Tyms (Myc-DDK-tagged) - Mouse thymidylate synthase (cDNA clone MGC:28246 IMAGE:3994204) |
CNY 2,400.00 |
|
| MR204343L3 | Lenti ORF clone of Tyms (Myc-DDK-tagged) - Mouse thymidylate synthase (cDNA clone MGC:28246 IMAGE:3994204) |
CNY 4,750.00 |
|
| MR204343L4 | Lenti ORF clone of Tyms (mGFP-tagged) - Mouse thymidylate synthase (cDNA clone MGC:28246 IMAGE:3994204) |
CNY 4,750.00 |
