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Glis1 (BC066157) Mouse Untagged Clone
CAT＃: MC218609
Glis1 (untagged) - Mouse GLIS family zinc finger 1 (cDNA clone MGC:76505 IMAGE:30468539), (10ug)
CNY 4,608.00
CNY 6,940.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | Gli6, GliH1, Gli5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC218609 representing BC066157
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCAGCGCCCACACACCCCTGCCCACCCACTGCCGAGCCCCATCGTCCATGGGTCTGCCCTCAGACC TGGACTTTCCAGACCGAGGCCTCACCAACCCTGCACCTTCCTGCTACCTTCTGGGCAATGAACCCATCTC AGACCTGGGTCCCCAACCCGAGGCCCACCTCCCCGAGGGCAGCCTGAAACGCTGCTGCCTCCTGGGCCTG CCCCCCACCTCTTCAGCCTCCTCCTCACCCTGTGCCTCCTCAGATATCAATCCTGTCATCCACTCCTCCC AGACAGCTCTAGTTAGCTGTGTAAATGGACTCCGAAGCCCACCTCTGCCGGGAGACCTGGGGGGCCCTCC CAAGCGGTCACGGCCCGGGCCTGCATCCAGTGACGGCCAGGAGGGCAGCTTGCAGCTTGAAGCATGCCGG AAGTCAGGCTTCCTGAAGCAGGAGCCCATGGACGAGTTTTCAGAGCTTTTTGCTCCACACCACCAGGGTT TGCCACCCCCTTACCCCTTGCCTCAGTTGCCAACTGGCCCCGGCCTCGGAGGCCTAGGGCTGGGCCTGGC AGGTAGGATGGTTGCCGGTCGGCAGGCATGCCGCTGGGTGGACTGCTGCGCAGCCTACGGGCAGCAGGAG GAGCTGGAGCGGCACATCGAGAAGAGCCACATCGACCAGCGCAAGGGCGAAGACTTCACCTGCTTCTGGG CCGGGTGTGTGCGGCGCTACAAGCCCTTCAATGCCCGCTACAAGCTGCTCATCCACATGAGGGTACACTC AGGCGAGAAGCCCAACAAGTGCATGTTCGAAGGCTGCAGTAAAGCCTTTTCCCGTCTGGAGAACCTGAAG ATCCATCTGCGGAGCCACACAGGCGAGAAACCATACCTGTGCCAGCACCCAGGCTGCCAGAAGGCCTTCA GCAACTCCAGCGACCGTGCCAAGCACCAACGCACCCACCTCGACACGAAGCCATATGCTTGTCAGATCCC TGGCTGCTCCAAGCGCTACACGGACCCCAGCTCCCTCCGCAAGCACGTGAAGGCCCACTCAGCCAAAGAG CAGCAGGTGCGTAAGAAGCTGCACACAGGTGCCGACCCAGAGGCTGATGTTCTGTCCGAGTGTCTGTCCC TGCAGCAGCTCCAAGCATCCACACTGTTGCCGGCCAGCAGAGGGAAGGGCAGCCAAACCCTGAGCCAGGA GCTCCTCCCAGGTGTGTATCCTGGCTCCGTCACCCCACAAAACGGGCTTGCTTCAGGCATCCTGTCCCCC TCCCACGATGTCCCTTCCAGGCACCACCCACTGGAGGTCCCCACTGGTTCCCACCACCACCTGTCCCCTC TGCCCACAGCTGAGAGCACCAGGGATGGCCTGGGGCCCAGTCTCCTTTCACCCATGGCCAGCCCACTGAA GGGGCTTGGTCCCCCACCGCTACCACCAGCCTCCCAGAGTCAGTCTCCAGGGGGACAGTCATTCTCTACA GTCCCCAGCAAGCCTACCTCCCCATCCTTCCAAAGCCCACCACCTCTGCCCAGCCCCCAAGGCTACCAAG GCAGTTTCCATTCCATCCAGAACTGCTTCCCCTACGCTGACTGCTACCGGGCCACTGAGCCAGCAGCCTC CAGGGATGGACTGGTGGGTGATGCCCACGGTTTCAACCCCTTGCGACCCAGCACATACTCCAGCCTCAGC ACACCTTTATCCGCACCAGGCTACGAGACCCTGGCAGAAACGCCGTGTCCCCCAGCGCTGCAGCCACAGC CAGCTGAAGACCTGGTACCTAGTGGTCCTGAGGACTGTGGCTTCTTCCCCAATGGGGCCTTTGACCACTG TCTGAGTCACATCCCGTCCATCTACACTGACACCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | BC066157 |
| Insert Size | 1857 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC066157, AAH66157 |
| RefSeq Size | 2431 bp |
| RefSeq ORF | 1856 bp |
| Locus ID | 230587 |
| Gene Summary | Acts as both a repressor and activator of transcription (PubMed:12042312, PubMed:12385751, PubMed:21654807). Binds to the consensus sequence 5'-GACCACCCAC-3' (PubMed:12042312). By controlling the expression of genes involved in cell differentiation inhibits the lineage commitment of multipotent cells (PubMed:21654807, PubMed:30544251). Prevents, for instance, the differentiation of multipotent mesenchymal cells into adipocyte and osteoblast (PubMed:30544251).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209472 | Glis1 (tGFP-tagged) - Mouse GLIS family zinc finger 1 (cDNA clone MGC:76505 IMAGE:30468539) |
CNY 3,900.00 |
|
| MR209472 | Glis1 (Myc-DDK-tagged) - Mouse GLIS family zinc finger 1 (cDNA clone MGC:76505 IMAGE:30468539) |
CNY 4,600.00 |
|
| MR209472L3 | Lenti ORF clone of Glis1 (Myc-DDK-tagged) - Mouse GLIS family zinc finger 1 (cDNA clone MGC:76505 IMAGE:30468539) |
CNY 5,420.00 |
|
| MR209472L4 | Lenti ORF clone of Glis1 (mGFP-tagged) - Mouse GLIS family zinc finger 1 (cDNA clone MGC:76505 IMAGE:30468539) |
CNY 5,420.00 |
