Atg9a (BC079884) Mouse Untagged Clone
CAT#: MC218655
Atg9a (untagged) - Mouse autophagy-related 9A (yeast) (cDNA clone MGC:105176 IMAGE:6840050), (10ug)
CNY 6,940.00
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Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | Atg9, Atg9l1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>BC079884
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACAGTTTGACACTGAATACCAGCGCCTAGAGGCCTCCTATAGCGATTCGCCCCCCGGGGAAGAGG ACCTGTTGGTGCATGTGGCCGAGGGGAGCAAATCACCTTGGCACCACATTGAAAACCTTGACCTCTTCTT CTCTCGAGTTTATAATCTCCACCAGAAGAATGGCTTCACATGTATGCTCATTGGAGAGATGTTTGAACTC ATGCAGTTCCTCTTTGTGGTTGCCTTCACCACCTTCCTGGTTAGCTGTGTGGACTATGACATCCTCTTTG CCAACAAGATGGTGAACCACAGTCTCCATCCTACTGAGCCTGTCAAGGTCACTCTGCCAGATGCCTTTTT GCCTGCCCAAGTCTGTAGTGCCAGGATTCAGGAAAATGGCTCTCTTATCACCATCCTGGTCATTGCTGGT GTCTTCTGGATCCACCGGCTTATCAAGTTTATCTATAACATTTGCTGCTATTGGGAGATCCACTCCTTCT ACCTACATGCTCTTCGGATCCCAATGTCTGCCCTTCCATACTGCACATGGCAGGAAGTTCAGGCCCGGAT TGTGCAGACGCAGAAAGAGCATCAGATCTGCATCCACAAGCGTGAGCTGACAGAGTTGGACATCTACCAT CGCATCCTACGTTTCCAGAACTACATGGTGGCCCTGGTGAACAAATCCCTCCTGCCTCTGCGCTTCCGTC TGCCCGGCCTCGGAGAGGTTGTCTTCTTCACCCGTGGCCTCAAGTACAACTTTGAGCTCATCCTCTTCTG GGGACCCGGCTCTCTGTTTCTCAATGAGTGGAGCCTCAAGGCCGAGTACAAACGTGGAGGGCAACGGCTA GAGCTGGCCCAGCGTCTCAGCAACCGCATCTTGTGGATTGGCATCGCCAACTTCCTGCTGTGTCCCCTCA TCCTCATCTGGCAGATCCTCTATGCCTTCTTCAGCTATGCCGAGGTGCTGAAGAGAGAGCCGGGGGCCCT GGGAGCGCGTTGCTGGTCACTCTATGGCCGTTGTTACCTCCGCCACTTCAATGAGCTGGAGCATGAGCTG CAGTCCCGCCTCAACCGAGGCTACAAGCCCGCCTCCAAGTACATGAATTGCTTCTTGTCACCGCTGCTGA CTCTGCTGGCCAAGAATGGTGCCTTCTTCGCTGGCTCTATCCTGGCTGTGCTTATTGCCCTCACCATCTA TGATGAAGATGTGTTAGCTGTGGAACACGTCCTCACCACGGTCACCCTCCTGGGAGTCACGGTGACTGTG TGCAGGTCCTTCATCCCAGACCAGCACATGGTGTTCTGCCCCGAGCAGCTGCTCCGAGTGATTCTTGCAC ACATCCACTACATGCCTGACCACTGGCAGGGTGTTCATCTTGGAGGAGTTGCTGAGTCCCATCGTCACAC CCCTCATTCTCATCTTCTGCCTCCGCCCTCGGGCCCTGGAGATCATAGACTTCTTCCGCAACTTTACGGT CGAGGTCGTGGGTGTGGGAGACACCTGCTCCTTTGCTCAGATGGACGTTCGCCAGCATGGCCATCCTCAG TGGCTGTCTGGAGGGCAGACAGAGGCCTCAGTGTACCAGCAAGCCGAGGACGGGAAGACCGAGTTGTCGC TCATGCACTTTGCCATCACCAATCCCGGCTGGCAGCCCCCTCGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | BC079884 |
Insert Size | 1656 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC079884, AAH79884 |
RefSeq Size | 3792 bp |
RefSeq ORF | 1655 bp |
Locus ID | 245860 |
Gene Summary | Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H (By similarity). Required for carbonyl cyanide m-chlorophenylhydrazone (CCCP)-induced ATG8 family proteins lipidation, a key autophagy step.[UniProtKB/Swiss-Prot Function] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG208753 | Atg9a (tGFP-tagged) - Mouse autophagy-related 9A (yeast) (cDNA clone MGC:105176 IMAGE:6840050) |
CNY 3,420.00 |
|
MR208753 | Atg9a (Myc-DDK-tagged) - Mouse autophagy-related 9A (yeast) (cDNA clone MGC:105176 IMAGE:6840050) |
CNY 4,104.00 |
|
MR208753L1 | Lenti ORF clone of Atg9a (Myc-DDK-tagged) - Mouse autophagy-related 9A (yeast) (cDNA clone MGC:105176 IMAGE:6840050) |
CNY 5,040.00 |
|
MR208753L2 | Lenti ORF clone of Atg9a (mGFP-tagged) - Mouse autophagy-related 9A (yeast) (cDNA clone MGC:105176 IMAGE:6840050) |
CNY 5,040.00 |
|
MR208753L3 | Lenti ORF clone of Atg9a (Myc-DDK-tagged) - Mouse autophagy-related 9A (yeast) (cDNA clone MGC:105176 IMAGE:6840050) |
CNY 5,040.00 |
|
MR208753L4 | Lenti ORF clone of Atg9a (mGFP-tagged) - Mouse autophagy-related 9A (yeast) (cDNA clone MGC:105176 IMAGE:6840050) |
CNY 5,040.00 |