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Atg9a (BC079884) Mouse Tagged ORF Clone
CAT＃: MR208753
- TrueORF®
Atg9a (Myc-DDK-tagged) - Mouse autophagy-related 9A (yeast) (cDNA clone MGC:105176 IMAGE:6840050)
ORF Plasmid: tGFP
"BC079884" in other vectors (6)
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CNY 4,104.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Atg9, Atg9l1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208753 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCACAGTTTGACACTGAATACCAGCGCCTAGAGGCCTCCTATAGCGATTCGCCCCCCGGGGAAGAGG ACCTGTTGGTGCATGTGGCCGAGGGGAGCAAATCACCTTGGCACCACATTGAAAACCTTGACCTCTTCTT CTCTCGAGTTTATAATCTCCACCAGAAGAATGGCTTCACATGTATGCTCATTGGAGAGATGTTTGAACTC ATGCAGTTCCTCTTTGTGGTTGCCTTCACCACCTTCCTGGTTAGCTGTGTGGACTATGACATCCTCTTTG CCAACAAGATGGTGAACCACAGTCTCCATCCTACTGAGCCTGTCAAGGTCACTCTGCCAGATGCCTTTTT GCCTGCCCAAGTCTGTAGTGCCAGGATTCAGGAAAATGGCTCTCTTATCACCATCCTGGTCATTGCTGGT GTCTTCTGGATCCACCGGCTTATCAAGTTTATCTATAACATTTGCTGCTATTGGGAGATCCACTCCTTCT ACCTACATGCTCTTCGGATCCCAATGTCTGCCCTTCCATACTGCACATGGCAGGAAGTTCAGGCCCGGAT TGTGCAGACGCAGAAAGAGCATCAGATCTGCATCCACAAGCGTGAGCTGACAGAGTTGGACATCTACCAT CGCATCCTACGTTTCCAGAACTACATGGTGGCCCTGGTGAACAAATCCCTCCTGCCTCTGCGCTTCCGTC TGCCCGGCCTCGGAGAGGTTGTCTTCTTCACCCGTGGCCTCAAGTACAACTTTGAGCTCATCCTCTTCTG GGGACCCGGCTCTCTGTTTCTCAATGAGTGGAGCCTCAAGGCCGAGTACAAACGTGGAGGGCAACGGCTA GAGCTGGCCCAGCGTCTCAGCAACCGCATCTTGTGGATTGGCATCGCCAACTTCCTGCTGTGTCCCCTCA TCCTCATCTGGCAGATCCTCTATGCCTTCTTCAGCTATGCCGAGGTGCTGAAGAGAGAGCCGGGGGCCCT GGGAGCGCGTTGCTGGTCACTCTATGGCCGTTGTTACCTCCGCCACTTCAATGAGCTGGAGCATGAGCTG CAGTCCCGCCTCAACCGAGGCTACAAGCCCGCCTCCAAGTACATGAATTGCTTCTTGTCACCGCTGCTGA CTCTGCTGGCCAAGAATGGTGCCTTCTTCGCTGGCTCTATCCTGGCTGTGCTTATTGCCCTCACCATCTA TGATGAAGATGTGTTAGCTGTGGAACACGTCCTCACCACGGTCACCCTCCTGGGAGTCACGGTGACTGTG TGCAGGTCCTTCATCCCAGACCAGCACATGGTGTTCTGCCCCGAGCAGCTGCTCCGAGTGATTCTTGCAC ACATCCACTACATGCCTGACCACTGGCAGGGTGTTCATCTTGGAGGAGTTGCTGAGTCCCATCGTCACAC CCCTCATTCTCATCTTCTGCCTCCGCCCTCGGGCCCTGGAGATCATAGACTTCTTCCGCAACTTTACGGT CGAGGTCGTGGGTGTGGGAGACACCTGCTCCTTTGCTCAGATGGACGTTCGCCAGCATGGCCATCCTCAG TGGCTGTCTGGAGGGCAGACAGAGGCCTCAGTGTACCAGCAAGCCGAGGACGGGAAGACCGAGTTGTCGC TCATGCACTTTGCCATCACCAATCCCGGCTGGCAGCCCCCTCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208753 protein sequence
Red=Cloning site Green=Tags(s) MAQFDTEYQRLEASYSDSPPGEEDLLVHVAEGSKSPWHHIENLDLFFSRVYNLHQKNGFTCMLIGEMFEL MQFLFVVAFTTFLVSCVDYDILFANKMVNHSLHPTEPVKVTLPDAFLPAQVCSARIQENGSLITILVIAG VFWIHRLIKFIYNICCYWEIHSFYLHALRIPMSALPYCTWQEVQARIVQTQKEHQICIHKRELTELDIYH RILRFQNYMVALVNKSLLPLRFRLPGLGEVVFFTRGLKYNFELILFWGPGSLFLNEWSLKAEYKRGGQRL ELAQRLSNRILWIGIANFLLCPLILIWQILYAFFSYAEVLKREPGALGARCWSLYGRCYLRHFNELEHEL QSRLNRGYKPASKYMNCFLSPLLTLLAKNGAFFAGSILAVLIALTIYDEDVLAVEHVLTTVTLLGVTVTV CRSFIPDQHMVFCPEQLLRVILAHIHYMPDHWQGVHLGGVAESHRHTPHSHLLPPPSGPGDHRLLPQLYG RGRGCGRHLLLCSDGRSPAWPSSVAVWRADRGLSVPASRGREDRVVAHALCHHQSRLAAPS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC079884 |
| ORF Size | 1653 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC079884, AAH79884 |
| RefSeq Size | 3792 bp |
| RefSeq ORF | 1655 bp |
| Locus ID | 245860 |
| MW | 63.1 kDa |
| Gene Summary | Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H (By similarity). Required for carbonyl cyanide m-chlorophenylhydrazone (CCCP)-induced ATG8 family proteins lipidation, a key autophagy step.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Macrophage scavenger receptor 1 controls Chikungunya virus infection through autophagy in mice
,null,
Communications Biology
,PubMed ID 33033362
[Atg9a]
|
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218655 | Atg9a (untagged) - Mouse autophagy-related 9A (yeast) (cDNA clone MGC:105176 IMAGE:6840050), (10ug) |
CNY 6,940.00 |
|
| MG208753 | Atg9a (tGFP-tagged) - Mouse autophagy-related 9A (yeast) (cDNA clone MGC:105176 IMAGE:6840050) |
CNY 3,420.00 |
|
| MR208753L1 | Lenti ORF clone of Atg9a (Myc-DDK-tagged) - Mouse autophagy-related 9A (yeast) (cDNA clone MGC:105176 IMAGE:6840050) |
CNY 5,040.00 |
|
| MR208753L2 | Lenti ORF clone of Atg9a (mGFP-tagged) - Mouse autophagy-related 9A (yeast) (cDNA clone MGC:105176 IMAGE:6840050) |
CNY 5,040.00 |
|
| MR208753L3 | Lenti ORF clone of Atg9a (Myc-DDK-tagged) - Mouse autophagy-related 9A (yeast) (cDNA clone MGC:105176 IMAGE:6840050) |
CNY 5,040.00 |
|
| MR208753L4 | Lenti ORF clone of Atg9a (mGFP-tagged) - Mouse autophagy-related 9A (yeast) (cDNA clone MGC:105176 IMAGE:6840050) |
CNY 5,040.00 |
