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Nt5e (NM_011851) Mouse Untagged Clone
CAT＃: MC219176
Nt5e (untagged) - Mouse 5' nucleotidase, ecto (Nt5e), (10ug)
CNY 6,440.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 5'-NT; 2210401F01Rik; AI447961; CD73; eNT; NT; Nt5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC219176 representing NM_011851
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGTCCCGCGGCCGCTAAGGTACCCAAGTGGCTGCTTCTCGCACTGAGCGCTCTACTACCACAGTGGC CCGCAGCCAGTGCCTGGGAGCTCACGATCCTGCACACAAACGACGTGCACAGCCGGCTAGAGCAGACCAG CGATGACTCCACCAAGTGCCTCAACGCCAGCCTGTGTGTGGGCGGCGTGGCCCGGCTCTTTACCAAGGTG CAGCAGATCCGCAAGGAAGAACCCAACGTGCTGTTTTTGGATGCCGGAGACCAGTACCAGGGCACCATCT GGTTCACCGTTTACAAAGGCCTTGAAGTGGCACACTTCATGAACATCCTGGGCTACGATGCTATGGCACT GGGAAATCATGAATTTGATAACGGTGTGGAAGGACTGATTGATCCCCTCCTCAGAAACGTTAAATTTCCA ATTCTGAGCGCAAACATTAAGGCACGGGGGCCTCTAGCACATCAGATATCTGGACTTTTTCTGCCATCTA AAGTTCTCTCTGTTGGCGGTGAGGTTGTGGGGATTGTTGGATATACTTCAAAGGAAACCCCTTTCCTCTC AAATCCAGGGACAAATTTAGTCTTTGAAGATGAAATCTCTGCATTGCAGCCTGAAGTAGATAAACTAAAG ACGCTAAATGTGAATAAGATCATCGCCCTGGGGCACTCTGGTTTTGAGATGGACAAACTTATCGCTCAGA AAGTTCGAGGTGTGGACATCGTGGTGGGAGGACACTCCAACACCTTTCTCTACACAGGAAATCCACCTTC CAAAGAAGTGCCTGCGGGGAAGTACCCATTCATAGTCACCGCAGATGATGGACGGCAGGTGCCTGTGGTC CAGGCCTATGCCTTTGGCAAATACCTGGGCTACCTGAAGGTTGAGTTTGATGATAAAGGCAATGTTATCA CTTCCTATGGAAATCCCATTCTTCTCAACAGCAGCATTCCTGAAGATGCGACCATCAAAGCAGACATTAA CCAATGGAGGATAAAATTAGATAATTATTCTACCCAGGAACTCGGGAGAACGATCGTCTACCTGGATGGC TCCACTCAGACGTGCCGCTTCAGGGAATGCAACATGGGAAACCTGATCTGTGATGCCATGATTAACAACA ACCTCAGACACCCAGATGAAATGTTTTGGAACCACGTGTCCATGTGCATTGTAAACGGAGGTGGCATCCG GTCCCCCATTGATGAGAAGAACAATGGTACCATCACCTGGGAGAACCTGGCTGCTGTGCTGCCCTTTGGA GGGACATTTGACCTCGTCCAATTAAAAGGGTCCACCCTGAAGAAGGCTTTTGAGCACAGCGTGCATCGCT ATGGCCAGTCCACCGGAGAGTTCCTGCAAGTGGGTGGAATCCATGTGGTGTACGATATTAACCGAAAGCC CTGGAACAGAGTGGTCCAATTAGAAGTTCTCTGCACCAAGTGTCGAGTGCCCATCTATGAGCCTCTTGAA ATGGATAAAGTGTATAAAGTGACCCTCCCAAGCTATCTGGCCAACGGTGGAGATGGATTCCAGATGATAA AAGATGAATTACTAAAGCATGACTCTGGTGATCAAGATATCAGCGTGGTTTCTGAATACATCTCAAAAAT GAAAGTAGTTTACCCAGCCGTTGAAGGGCGGATCAAGTTCTCTGCAGCAAGTCATTACCAGGGAAGCTTT CCTTTAGTAATTCTTTCATTTTGGGCAATGATCCTTATTTTGTACCAATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_011851 |
| Insert Size | 1731 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC119268, AAI19269 |
| RefSeq Size | 2602 bp |
| RefSeq ORF | 1731 bp |
| Locus ID | 23959 |
| UniProt ID | Q61503 |
| Gene Summary | This gene encodes a membrane-bound nucleotidase that hydrolyzes extracellular nucleoside monophosphates. The encoded preproprotein undergoes proteolytic processing to generate to a functional, homodimeric enzyme that preferentially uses adenosine monophosphate as a substrate to generate free adenosine. Mice lacking the encoded protein exhibit a significantly reduced fall in stop flow pressure and superficial nephron glomerular filtration rate in response to a saturating increase of tubular perfusion flow. [provided by RefSeq, Sep 2016] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG227439 | Nt5e (tGFP-tagged) - Mouse 5' nucleotidase ecto (Nt5e), (10ug) |
CNY 8,032.00 |
|
| MR227439 | Nt5e (Myc-DDK-tagged) - Mouse 5' nucleotidase, ecto (Nt5e) |
CNY 6,432.00 |
|
| MR227439L1 | Lenti ORF clone of Nt5e (Myc-DDK-tagged) - Mouse 5' nucleotidase, ecto (Nt5e) |
CNY 7,600.00 |
|
| MR227439L2 | Lenti ORF clone of Nt5e (mGFP-tagged) - Mouse 5' nucleotidase, ecto (Nt5e) |
CNY 8,832.00 |
|
| MR227439L3 | Lenti ORF clone of Nt5e (Myc-DDK-tagged) - Mouse 5' nucleotidase, ecto (Nt5e) |
CNY 8,832.00 |
|
| MR227439L4 | Lenti ORF clone of Nt5e (mGFP-tagged) - Mouse 5' nucleotidase, ecto (Nt5e) |
CNY 8,832.00 |
