Sppl2b (NM_175195) Mouse Untagged Clone

CAT#: MC219200

Sppl2b (untagged) - Mouse RIKEN cDNA 3110056O03 gene (3110056O03Rik), (10ug)



  "NM_175195" in other vectors (4)

CNY 7,320.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 3110056O03Rik; AW550292; PSL1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC219200 representing NM_175195
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCGGCGCGGCTGGCCGCAGCTTTGCTGCTACTCGCGGCCCAGGTGGCCTGTGAGTTCGGCGTGC
TTCGAGTGGTGTCCCAAACCAGTAGGACTCGGAGCAGGGACTACTGCATCCTCTACAACCCACAGTGGGC
CCACTTGCCACATGACCTCAGCAAAGTGTCTCTCCTGAAGCTTCGAGACTTGAGCACCACCCAGCTCTGT
TCCTACCTTGACGTCCCTGCAGAAGACTTCACCAACCAAATCGCACTGGTGGCCCGGGGCAACTGTACCT
TCTATGAGAAAGTGCGGCTGGCCCAGGGCAGTGGGGCTCACGGGCTGCTGATAGTCAGCAAGGAGAAGCT
GGTCCCTCCAGGAGGCAACAAGACACAGTATGAGGAGATAAGCATCCCTGTGGCCCTGCTGAGCCACAGA
GACCTTCAAGACATCTTCAGGCGCTTTGGCCGTGAGGTAATGGTGGCACTGTATGCTCCGAGCGAGCCAG
TGATGGACTACAACATGGTCATCATCTTTGTCATGGCCGTGGGCACCGTCGCCATCGGTGGCTACTGGGC
TGGCAGCCATGATGTGAAGAAGTACATGAAGCATAAGCGAGACGATGGTCCTGAGAAACAGGAGGATGAG
GCGGTGGACGTGACGCCAGTCATGATCTGCGTGTTTGTTGTAATGTGCTGCTTCATGCTTGTGCTTCTCT
ACTACTTCTATGACCGCCTGGTCTACGTGATCATTGGAATCTTCTGCCTGGCCTCCTCCACCGGTCTCTA
CAGCTGTCTGGCACCCTTTGTGCGGAAGCTGCCCTTCTGCACGTGCAGGGTCCCCGACAACAACCTGCCG
TACTTCCACAAGCGCCCACAGGCCCGCATGCTGCTGCTGGCACTCTTCTGTGTCACTGTCTCCGTGGTGT
GGGGTATCTTCCGAAATGAGGACCAGTGGGCATGGGTCCTGCAAGACACCCTGGGCATTGCCTTCTGCCT
GTACATGCTGAAGACCATCCGCCTGCCTACCTTCAAGGCCTGCACGCTGCTGCTGCTGGTGCTCTTCATC
TACGACATCTTCTTTGTCTTCATCACCCCCTTCCTGACCAAGAGTGGCAACAGCATCATGGTGGAGGTGG
CCACCGGGCCCTCGAACTCATCCACCCATGAGAAGCTGCCCATGGTGCTGAAGGTGCCCAGGCTGAACAC
CTCGCCACTCTCCCTGTGTGACCGGCCCTTCTCCCTCCTGGGCTTTGGAGACATCTTGGTGCCAGGGCTG
CTGGTGGCCTACTGTCACAGGTTTGATATCCAGGTGCAGTCCTCCAGAATTTACTTTGTGGCCTGTACCA
TTGCCTATGGCCTGGGCCTCCTGGTGACCTTCGTGGCTCTTGTCCTCATGCAGCGTGGCCAGCCTGCACT
ACTCTACCTGGTCCCTTGCACACTGCTGACCAGCTGCACCGTGGCTCTGTGGCGCCGGGAGCTGGGTGCC
TTCTGGACGGGCAGCGGTTTTGCGAAGGATGCACCTCAGACCCCGTGGGCCGCAACCCAGGGACCTGTGC
CTCCGAAGGCTGTGGGTTCCTCCCTCTCCGAGCAGCCTCCAAGTGAAGAGCTAGCCAAGAGCCCCCTGTC
CACTGAGGAGGCCGGAGCCGCAGACCCTGCCAAGGATCCTGACAACCCTGTGGCCAGTCCCCTCAGCCCC
TCAAATGGGGATGAGGCTCAGCCTATCCCTGTGGTGAAGCCAGAGACTTCGGCCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_175195
Insert Size 1737 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_175195.3, NP_780404.2
RefSeq Size 2887 bp
RefSeq ORF 1737 bp
Locus ID 73218
UniProt ID Q3TD49
Gene Summary Intramembrane-cleaving aspartic protease (I-CLiP) that cleaves type II membrane signal peptides in the hydrophobic plane of the membrane. Functions in ITM2B and TNF processing. Catalyzes the intramembrane cleavage of the anchored fragment of shed TNF-alpha (TNF), which promotes the release of the intracellular domain (ICD) for signaling to the nucleus. May play a role in the regulation of innate and adaptive immunity.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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