Dach2 (NM_001142570) Mouse Untagged Clone

CAT#: MC219643

Dach2 (untagged) - Mouse dachshund 2 (Drosophila) (Dach2), transcript variant 1, (10ug)



  "NM_001142570" in other vectors (3)

CNY 7,790.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
Goat Polyclonal Antibody against DACH2
    • 100 ug

CNY 5,371.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 9430028N04Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC219643 representing NM_001142570
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGTCTCTGCACCTCCGGTGATCTCTGCAACTTCCAGCAGCGCCGGCGTCCCGGGGGGTTTATTCC
GGGCTGAACCCCTGTATTCGTCTCCAGGAGAGCCCCCTCGCCTAACCCCTAACATGATCAACAGTTTCAT
GGCCAATAACCATAACGGCAGTGTCTTGGGTGGCGGGATTGGTGGTGGCAGCGGTGGCAGCAGCAACACC
AACACAAACGAGTGCCGGATGGTCGACATGCACGGGGTGAAGGTAGCTTCATTCCTGATGGATGGCCAGG
AACTGATCTGCCTGCCGCAAGTCTTTGACCTTTTCCTCAAGCACCTGGTGGGAGGGTTGCACACAGTGTA
CACTAAGCTGAAGAGACTCGACATATCTCCGGTGGTGTGCACTGTAGAGCAAGTTCGAATCCTCCGTGGG
CTGGGGGCCATCCAGCCAGGGGTGAATCGTTGCAAGCTCATCACCAGGAAAGACTTTGAAACTTTGTTCA
CCGATTGCACCAACGCCAGTTCAAGGCCTGGCAGGCCCCCTAAGCGTTCTTTGGGAGTATTGCAAGATAA
TGCCCGACTTCTGCCCCATGCAGTCCCAGGCCTCTTATCACCAGGACTGATCACTCCAACAGGTATCACA
GCTGCAGCGATGGCTGAGGCCATGAAGCTCCAGAAGATGAAGCTTATGGCAATGAATACTCTTCAGGGTA
ATGGAAGCCAAAATGGGACTGAGTCAGAGCCAGATGACCTTAATTCTACGACAGGTGGAAGTGAATCCTC
CTGGGATAAAGATAAGATACAATCTCCACTTGCTGCTTCTGGACCTCAACATGGAATTGCTCATGCAGCA
CTTGCTGGTCAGCCAGGCCTTGGAGGTGCTCCGACCCTTAATCCACTTCAGCAGAATCACCTGCTAAGCA
ATCGTCTGGATCTCCCATTTATGATGATGCCTCATCCCCTACTTCCAGTCAGCTTACCTCCTGCATCAGT
TGCCATGGCAATGAATCAGATGAATCATCTCAATACTATTGCCAACATGGCTGCTGCAGCCCAGATTCAC
AGTCCACTCTCCAGAGCTGGTGCCTCTGTTATAAAGGAACGGATCCCAGAGAGTCCTTCTCCTGCTCCCT
CTCTGGAAGAGAGTCATCGTCCTGGGAGCCAGACCTCCTCCCACCCAAGCAGCAGTGTGTCCAGCTCTCC
CTCGCAGATGGATCATCATTCAGAGAGAATGGTTATGATGCCCAACAATCGAGAAGAGCTTATTGTTGAC
CAAGATAATGGACAAAGCATAAAAAAATTCCAGAGGGATAATAAAGAAGAAGTACCAGCTCAAATCCCAG
TCATGAAGTCACCCTTGGATAAGATCCAGTTGGCTCCTGGACAGGCATTGCATCCTGGATTCCCTGGACC
ATTCATTTTTGCAGATAGTCTATCTTCTGTGGAGACTCTGTTGACCAACATTCAGGGTCTACTGAAAGTG
GCTTTGGATAATGCTCGTATCCAGGAGAAGCAGATTCAGCAGGAAAAGAAGGAACTGCGAATAGAGCTCT
TCAGAGAAAGAGAAATTAGAGAAAACCTGGAGCGACAACTTGCAGTTGAGCTTCAAAGCAGAAGTACAAT
GCAAAAGCGTCTGAAAAAAGAGAAAAAGGCTAAGAGAAAACTTCAGGAAGCCTTGGAATTTGAATCAAAG
CGCCGCGAGCAAGTGGAGCAAGCACTTAAACAAGCTACATCTGGTGACAGTGGACTGAGAATGTTAAAAG
ATTCTGGAATTCCAGATATTGAAATAGAAAACAGTGGAACGCCGCATGACAGTGCTGCTATGCAAGCCTG
A


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001142570
Insert Size 1821 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001142570.1, NP_001136042.1
RefSeq Size 5991 bp
RefSeq ORF 1821 bp
Locus ID 93837
UniProt ID Q925Q8
Gene Summary Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator for SIX1 and SIX6. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Is recruited with SIX6 to the p27Kip1 promoter in embryonal retina. SIX6 corepression seems also to involve NCOR1, TBL1, HDAC1 and HDAC3. May be involved together with PAX3, SIX1, and EYA2 in regulation of myogenesis. In the developing somite, expression of DACH2 and PAX3 is regulated by the overlying ectoderm, and DACH2 and PAX3 positively regulate each other's expression. Probably binds to DNA via its DACHbox-N domain.[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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