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Edem1 (NM_138677) Mouse Untagged Clone
CAT＃: MC220111
Edem1 (untagged) - Mouse ER degradation enhancer, mannosidase alpha-like 1 (Edem1), (10ug)
CNY 8,360.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | A130059K23Rik; EDEM; mKIAA0212 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC220111 representing NM_138677
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAATGGCGAGCGCTTGTCCTGGGGCTGGTGCTGCTGCGCCTCGGCCTCCACGCGGTGCTCTGGTTGG TCTTCGGGCTGGGGCCCAGCATGGGCTTCTACCAGCGGTTTCCGCTCAGCTTCGGCTTCCAGCGCTTGAG GGACCCCGACGGCTCGGGGCCAGTTGGTCCGCCCGGAGGCCCGGCCTGGCTTCATCGGCCAAGGCGCGGA ACGGAGGGGCGGCTTGAGACCCCGCCAGAGCCGGGACCGACGCCGGGACCGGGCGTGTGCGGCCCGGCGC ACTGGGGCTATGCCCTGGGCGGCGGTGGCTGCGGCCCGGACGAGTACGAGCGGCGCTACAGCGGCGCCTT CCCGCCGCAGCTGCGTGCCCAGATGCGCGACCTGGCGCGGGGCATGTTCGTCTTCGGCTATGACAACTAC ATGGCGCACGCCTTTCCGCAGGACGAGCTCAACCCCATCTACTGCCGCGGCCGGGGGCCTGACCGCGGAG ACCCTTCCAATCTGAACATCAATGATGTCCTAGGGAATTACTCTCTGACTCTGGTTGATGCCTTGGATAC ATTGGCAATAATGGGAAATTCATCCGAGTTCCAGAAGGCAGTCAAGTTAGTGATCAACACTGTTTCATTT GACAAAGATTCCACAGTCCAGGTCTTCGAAGCTACGATAAGGGTTCTGGGAAGCCTCCTTTCTGCTCACA GAATAATAACTGATTCCAAACAGCCCTTTGGTGACATGACAATTGAGGATTATGATAATGAATTGTTGTA CATGGCCCATGACTTGGCTGTGCGGCTCCTTCCAGCCTTTGAAAACACCAAGACAGGGATCCCCTATCCT CGGGTGAATCTGAAGACAGGTGTTCCTCCTGACAGCAATAATGAGACCTGCACAGCGGGGGCCGGTTCTC TCCTGGTGGAATTTGGGATTCTGAGCCGACTGCTGGGGGATTCCACTTTTGAGTGGGTTGCCAGACGAGC TGTGAAAGCCCTCTGGAACTTGCGGAGCAACGATACAGGATTATTAGGCAATGTTGTGAACATCCAGACA GGCCATTGGGTTGGAAAGCAGAGTGGCTTGGGTGCTGGGCTGGATTCCTTCTATGAGTACCTCTTGAAAT CTTACATTCTTTTTGGAGAAAAAGAAGATCTAGAGATGTTTAATGCTGCGTATCAGAGCATCCAGAGCTA CCTGCGAAGAGGCCGGGAAGCCTGCAATGAAGGAGAAGGAGACCCACCGCTCTACGTCAACGTGAACATG TTCAGTGGGCAGCTCATGAACACCTGGATTGACTCGCTGCAGGCTTTCTTCCCTGGACTGCAGGTTCTGA TAGGGGATGTGGAAGATGCCATCTGCCTACACGCCTTCTACTATGCCATATGGAAGCGGTACGGGGCCCT CCCTGAGCGCTATAACTGGCAACTCCAGGCCCCTGATGTTCTCTTCTACCCTCTGAGACCAGAGCTAGTG GAGTCCACATATCTCCTCTACCAGGCAACCAAGAATCCCTTCTACCTCCATGTAGGAATGGACATTCTGC AGAGTCTTGAAAAATACACAAAAGTCAAATGTGGATATGCTACGCTGCATCACGTCATAGACAAGTCTAA AGAAGACCGGATGGAAAGCTTCTTTCTCAGCGAGACTTGCAAATACTTGTATCTGCTATTTGATGAAGAG AATCCAGTACACAAATCTGGAACCAGATACATGTTTACAACTGAGGGCCATATCATATCTGTGGACAAAC GTCTTCGGGAATTGCCTTGGAAGGAATTCTTCTCTGAAGATGGGGAGCGGGACCAAGAGGAAAAGTTTGT ACACAGACCTAAGTCTCAGGAGCTCAGAGTCATTAACTCCAGTTCTAATTGTAATCGTGTTCCTGATGAG AGGAGATACTCCTTGCCCTTAAAGAGCATCTACATGCGCCAGATCGACCAGATGGTTGGCTTGATTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_138677 |
| Insert Size | 1959 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_138677.2, NP_619618.1 |
| RefSeq Size | 5858 bp |
| RefSeq ORF | 1959 bp |
| Locus ID | 192193 |
| UniProt ID | Q925U4 |
| Gene Summary | Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-independent manner, probably by forming a complex with SEL1L. It has low mannosidase activity, catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG209777 | Edem1 (tGFP-tagged) - Mouse ER degradation enhancer, mannosidase alpha-like 1 (Edem1) |
CNY 6,270.00 |
|
| MR209777 | Edem1 (Myc-DDK-tagged) - Mouse ER degradation enhancer, mannosidase alpha-like 1 (Edem1) |
CNY 4,856.00 |
|
| MR209777L3 | Lenti ORF clone of Edem1 (Myc-DDK-tagged) - Mouse ER degradation enhancer, mannosidase alpha-like 1 (Edem1) |
CNY 7,600.00 |
|
| MR209777L4 | Lenti ORF clone of Edem1 (mGFP-tagged) - Mouse ER degradation enhancer, mannosidase alpha-like 1 (Edem1) |
CNY 7,600.00 |
