Polh (NM_030715) Mouse Untagged Clone

CAT#: MC220523

Polh (untagged) - Mouse polymerase (DNA directed), eta (RAD 30 related) (Polh), (10ug)



  "NM_030715" in other vectors (4)

CNY 8,840.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms RAD30A; XPV
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC220523 representing NM_030715
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCCTGGGCAGAATCGAGTGGTTGCTCTTGTAGACATGGACTGCTTTTTTGTACAAGTGGAACAGC
GGCAAAATCCTCATTTGAGGAATAAACCTTGTGCAGTTGTACAGTACAAATCATGGAAAGGTGGTGGAAT
TATTGCAGTTAGCTATGAAGCCCGAGCATTTGGTGTCACTAGAAACATGTGGGCGGATGATGCTAAGAAG
TTATGTCCAGATCTTCTCCTGGCACAAGTTCGTGAGTCCCGTGGAAAAGCTAACCTCACCAAGTACCGGG
AAGCTAGTGTTGAAGTGATGGAGATAATGTCTTATTTTGCTGTGATTGAACGTGCCAGCATCGATGAGGC
TTATATAGATTTGACCAGTGCTGTACAAGAAAGACTTCAAAAGTTACAAGGTCAGCCTATCTCAGCAGAT
TTGCTGCCAAGCACTTACATTGAAGGGTTGCCCCGAGGCCCTACAGTAGAAGAGACTGTTCAGAAAGAGG
CTATTCGGAAACAAGGCTTGCTTCAGTGGCTTGATTCTCTTCAGAGTGATGATCCCACCTCTCCAGACCT
CAGGCTGACTGTGGGAGCCATGATTGTGGAAGAAATGAGAGCAGCCATAGAGAGTAAGACTGGCTTCCAG
TGTTCAGCTGGAATCTCACACAATAAGGTCCTGGCAAAACTTGCTTGTGGACTAAATAAGCCCAACCGCC
AAACCCTGGTCTCACATGGGTCAGTGCCACAGCTCTTCAGCCAGATGCCAATCCGTAAAATCCGAAGTCT
TGGAGGAAAGCTAGGGGCTTCTGTCATTGAAGTCCTTGGGATAGAATACATGGGTGACCTGACCCAGTTC
ACTGAATCCCAGCTCCAGAGTCATTTTGGAGAGAAGAATGGGTCTTGGCTGTATGCCATGTGTCGAGGTA
TTGAACACGATCCAGTTAAACCCAGGCAGTTACCTAAAACTATTGGCTGCAGCAAGAACTTCCCAGGGAA
AACAGCTCTGGCTACTCGGGAGCAGGTACAGTGGTGGCTTTTGCAGTTAGCTCTGGAACTTGAGGAGAGG
CTAACCAAAGATCGAAATGATAATGACCGCGTGGCCACTCAGCTGGTCGTCAGTATTCGTTTTCAGGGAG
ACAGGCGTCTCAGCAGCCTGCGGCGGTGCTGTGCCCTCCCTCGCTATGACGCTCACAAGATGAGCCAGGA
CGCATTTGCTGCCATCAGGAACTGTAATACATCTGGAATCCAAACTGAGTGGTCCCCTCCTCTCACAATG
CTCTTCCTCTGCGCTACCAAGTTCTCTGCGGCTGCCCCTCCAGCTTGCACGGATATCACTGCGTTCCTGA
GTAGCGACTCAAGTTGTCAGCCAAAGGTGCCAATTGCCAGCTCTGAGACTAGGACCCAGGGGTCTGGCCC
AGCTGTACCAACCTCTAAAGAGGCAGCCACATCTCTAGCGTCTTTTTTTCAAAAAGCTGCCAAAAAGCAG
AGAATGAAAGAGACTTCCTTTGTACCTCTTAACACAGCCACAGAGAAGTTATCATCCAAGCCTTCACTTG
TGTTCCAAAGCAGCCAAACCACAGGAAGTCAGTCTTTCTTTAAGCAGAAGAGCCTGCTGCTCCAGCACAC
ACAGCTTAGTAACTCTGCGGCTCCTGACCCTCCACAGGCCAGCCCTGCAGCGCAACCAAGCTGTCTGCCA
GCAGAGTGTGTAGACAGTGGTCCTGATGATGGAGCGGTGAAGCCAGTATCCTCTAAAGCAGTGTCTACAG
AAATGAATGTGGCTGGGGACAGTCCAAACGTGCTCGATTCTCCAGCTTACAACTCTCAGGAGGTGACCCA
AAGGGCCACTGAGGACCAAGTGCTCTGTGAGAAGTGTGACTCCCTGGTTCCAGTGTGGGACATGCCAGAA
CACACGGACTACCATTTTGCATTGGAGTTGCAGAAGTCTTTTTTGCAGCCTTGTACTTCAAAACCCCAGG
CTATTCCTGCCGTGTCTCCTCAGGGCAAAAGAAATCCCAAGAGTCCCTCGGCTTCCAGTAGTAAGCGCCT
TAGGCCCCATGGCATGCAGACGCTGGAATCGTTTTTTAAGCCACTGACACATTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_030715
Insert Size 2085 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_030715.3, NP_109640.1
RefSeq Size 2580 bp
RefSeq ORF 2085 bp
Locus ID 80905
UniProt ID Q9JJN0
Gene Summary DNA polymerase specifically involved in the DNA repair by translesion synthesis (TLS) (PubMed:10871396). Due to low processivity on both damaged and normal DNA, cooperates with the heterotetrameric (REV3L, REV7, POLD2 and POLD3) POLZ complex for complete bypass of DNA lesions. Inserts one or 2 nucleotide(s) opposite the lesion, the primer is further extended by the tetrameric POLZ complex. In the case of 1,2-intrastrand d(GpG)-cisplatin cross-link, inserts dCTP opposite the 3' guanine (By similarity). Particularly important for the repair of UV-induced pyrimidine dimers (PubMed:10871396). Although inserts the correct base, may cause base transitions and transversions depending upon the context. May play a role in hypermutation at immunoglobulin genes. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have any lyase activity, preventing the release of the 5'-deoxyribose phosphate (5'-dRP) residue. This covalent trapping of the enzyme by the 5'-dRP residue inhibits its DNA synthetic activity during base excision repair, thereby avoiding high incidence of mutagenesis. Targets POLI to replication foci (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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