Nolc1 (NM_001039353) Mouse Untagged Clone
CAT#: MC220594
Nolc1 (untagged) - Mouse nucleolar and coiled-body phosphoprotein 1 (Nolc1), transcript variant 4, (10ug)
CNY 9,030.00
货期*
5周
规格
Product images
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经常一起买 (3)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | NOPP130; NOPP140; P130 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC220594 representing NM_001039353
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGATACCGGCTTGCGCCGCGTGGTTCCCAGCGACCTTTATCCCCTTGTGCTCAGATTTCTGCGGG ATAGCCAACTCTCGGAGGTGGCCAGTAAATTTGCAAAAGCGACCGGCGCTACACAGCAGGACGCCAATGC CTCGTCCCTCTTGGACATCTATAGCTTCTGGCTCAAGTCCACCAAAGCCCCAAAGGTGAAGTTACAGTCA AATGGACCAGTGACCAAGAAGGCTAAGAAAGAGACTTCATCCAGTGACAGCAGTGAGGACAGCAGTGAGG ACGAGGACAAAAAAGCCCAGGGACTTCCCACACAGAAGGCTGCCGCACAGGTCAAGCGAGCCAGTGTGCC TCAGCATGCTGGAAAGGCAGCAGCCAAAGCTTCAGAGAGCAGCAGTAGTGAAGAATCCAGTGAGGAAGAG GAAGAGGACAAAAAGAAAAAGCCTGTCCAGCAGAAGGCAGCTAAGCCCCAAGCCAAGGCAGTCAGACCTC CTGCGAAGAAGGCAGAGAGCTCTGAGTCGGACTCAGACTCGGATTCGGACTCCAGCTCAGAGGAAGAAAC ACCACAGACCCAGAAGCCAAAGGCAGCTGTGGCAGCAAAAGCTCAGACTAAAGCCGAAGCCAAACCAGGT ACACCAGCGAAAGCACAGCCTAAGGTAGCCAATGGCAAAGCAGCCGCCAGCAGCAGCAGCAGCAGCAGCA GCGATGACTCAGAGGAAGAGAAGAAGGCAGCTGCACCTCCCAAGAAGACTGTACCAAAAAAGCAAGTCGT GGCCAAGGCCCCAGTGAAAGTAGCTGCCGCCCCCACCCAGAAGAGCTCCAGCAGTGAGGATTCTTCCAGT GAAGAGGAGGAGGGACAGAGACAACCCATGAAGAAAAAAGCAGGTCCCTACAGTTCAGTTCCACCACCCT CTGTTCCTTTACCAAAGAAGTCCCCGGGAACCCAGGCTCCAAAGAAAGCTGCTGCGCAGACACAGCCTGC AGACAGCAGTGACGACAGCAGTGACGATTCTGATTCAAGTTCTGAGGAAGAGAAAAAACCTCCAGCTAAG ACGGTCGTCTCCAAGACACCCGCCAAAGCAGCTCCAGTGAAGAAGAAAGCAGAAAGCTCTTCAGACAGCT CGGATTCTGACAGTTCTGAGGATGAAGCTCCTGCCAAGCCAGTCAGTACAACCAAGAGTCCCAAGCCAGC TGTCACTCCGAAGCCATCTGCAGCAAAGGCAGTGACAACTCCTAAGCAACCTGCAGGCAGTAACCAGAAA CCTCAGAGCAGGAAGGCTGACAGCAGCTCCAGCGAGGAGGAAAGCAGCTCCAGCGAGGAGGAGGAGGCCT CCAAGAAAAGTGCCACAACCCCCAAGGCCAAGGTGACTGCTAAAGCAGCACCCGCCAAACAGGCCCCTCA GGCTGCTGGGGACAGCAGCTCTGACTCAGATAGTTCCAGCAGTGAAGAGGAGGAGAAGACTCCTAAGCCC CCAGCTAAGAAGAAGGCAGCAGGTGGAGCCGTTTCTACACCAGCCCCTGGGAAGAAAGCAGAGGCCAAGA GCAGCAGCAGCAGCAGCAGCAGCAGCTCCGAAGATTCCAGTGAAGAGGAGAAAAAAAAGAAGCCCAAAGC TACTACCCCTAAAATACAGGCAAGCAAGGCCAATGGCACTCCAGCTTCTCTGAATGGAAAAGCAGCCAAG GAAAGTGAGGAGGAAGAGGAGGAGGAAGAAACAGAAGAGAAGAAAAAGGCAGCTGGGACCAAGCCAGGTT CAGGCAAAAAACGGAAGCAGAATGAGACCGCAGATGAAGCAACAACTCCTCAAGCTAAGAAAGTTAAGCT CGAGACCCCCAATACGTTTCCAAAAAGGAAGAAGGGAGAAAGAAGGGCGTCTTCCCCTTTCCGAAGGGTC AGGGAGGAGGAGATTGAGGTGGACTCTCGAGTGGCGGACAATTCCTTTGATGCCAAGCGAGGTGCAGCTG GAGACTGGGGGGAGCGAGCCAATCAGGTTCTGAAGTTCACCAAAGGCAAGTCCTTCCGGCATGAAAAAAC GAAGAAGAAGCGAGGCAGCTACCGGGGAGGCTCCATCTCTGTCCAGGTCAATTCCGTCAAATTCGACAGC GAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001039353 |
Insert Size | 2106 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001039353.2, NP_001034442.1 |
RefSeq Size | 3662 bp |
RefSeq ORF | 2106 bp |
Locus ID | 70769 |
UniProt ID | E9Q5C9 |
Gene Summary | Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (By similarity). Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (By similarity). Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus (PubMed:11424213). It has intrinsic GTPase and ATPase activities (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) uses an alternate in-frame splice site in the central coding region, compared to variant 3. The encoded isoform (D) is shorter, compared to isoform C. |
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