Diap3 (BC028920) Mouse Untagged Clone
CAT#: MC220799
Diap3 (untagged) - Mouse diaphanous homolog 3 (Drosophila) (cDNA clone MGC:30844 IMAGE:4008236), (10ug)
CNY 9,120.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | Dia2, mDia2, p134MDia2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>BC028920
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGACAAGAGGAGTCTTTCCTTGTTGGCAAAAGCCATGGATCCCAGGCAGCCCGCTATGATGGCAG ACGTGGTGAAGCTTCTGTCTGCAGTGTGCATTGTCGGCGAGGAAAGCATCCTTGAAGAAGTGTTAGAAGC CTTGACTTCAGCTGGAGAAGAAAGAAAGATTGACAGATTTTTTTCCATTGTGGAAGGCCTCCGGCATAAC TCAGTGAACCTGCAAGTTGCTTGTATGCAGCTCATAAATGCTCTCGTTACATCTCCTGATGATTTGGACT TCAGGCTTCACCTCAGAAATGAATTTATGCGTTGTGGATTGAAAGAGATATTGCCAAACTTAAAGGGCAT TAAGAATGATGGCCTGGATATACAACTTAAAGTCTTTGATGAGCACAAAGAAGAAGATTTGAGTGAGTTT TTCCATCGCCTTGAAGACATTAGAGCTGAACTTGATGAAGCATCTGATGTTTACAGCATGTTATGGGACA CAGTTAAGGAAACTCGAGCAGAGGGACATTTTCTTTCTATTCTTCAGCATCTCCTGCTCATTCGCAATGA TCGTTTTATAAGAGAGCAGTATTTCAAATTAATTGATGAGTGTGTGTCACAGATTGTGTTACATAGAGAT GGAACGGACCCTGACTTCACATACAGAAAAAGACTAGATTTGGACTTAAGTCAGTTTGTAGATGTTTGCA TAGATCAGGCCAAACTAGATGAGTGGGAAGAGAAAGCATCCGAACATTGCAAGAAATTTGAAAAAGAGTG TACTGACCACCAAGAAACCCAGGCTCAATTGCAGAAAAGAGAGGCAAAGATTAATGAGCTTCAAGCAGAG TTACAAGCTTTTAAATCCCAGTTTGGTGCCTTGCCACCTGGTACAAAAATTCCTTTGCAACCTTCAGTAG AAGGTGAAGCTGGCCCTTCAGCCCTTCCTCCTGCCCCACCAGCACTCAGTGGAGGAGTGCCGCCTCCCCC ACCGCCCCCTCCTCCACCACCCCCACCACTCCCAGGAATGCCAATGCCATTTGGTGGCCCTGTACCACCA CCACCTCCTCTGGGATTCCTGGGTGGGCAAAGCTCTATTCCATTAAACCTGCCATTTGGTTTGAAACCAA AGAAAGAATTTAAGCCTGAAATCAGCATGAGAAGATTGAATTGGTTAAAGATCGGACCAAATGAAATGTC TGAGAACTGCTTCTGGATCAAAGTAAATGAAAATAAGTATGAAAATAGGGATTTGCTTTGTAAACTTGAG AACACTTTTTGTTGCCAAGACAAAGAGAAAAGGAATACAAATGACTTTGATGAGAAGAAAGTTATTAAGA AGAGAATGAAGGAACTTAAATTTCTAGATCCTAAAATTGCTCAGAACCTTTCAATCTTCCTGAGCTCCTT CCGGGTGCCATATGAGAAAATCAGGACGATGATATTGGAAGTGGATGAAACACAGTTGTCAGAGTCCATG ATTCAGAACTTAATAAAGCACCTTCCTGATGAGGAGCAGTTGAAGTCATTGTCCCAGTTTAGAAGTGACT ATAACAGTTTGTGTGAGCCTGAGCAGTTCGCTGTTGTGATGAGCAATGTGAAGAGACTCCGGCCACGGCT CAGTGCTATTCTCTTTAAGCTTCAATTTGAAGAGCAGGTGAACAACATCAAACCTGACATCATGGCTGTC AGTACTGCCTGCGAGGAGATCAAGAAGAGCAAAGGCTTTAGCAAGTTGCTGGAACTTGTGTTGCTAATGG GAAACTACATGAATGCTGGCTCCCGGAATGCTCAAACCTTCGGATTTGACCTTAGCTCTCTCTGTAAACT GAAGGATACAAAATCTGCAGATCAGAAAACCACACTCCTCCATTTCCTGGTAGATGTATGTGAAGAAAAG CATGCTGACATCCTTCACTTTGTGGACGATTTGGCACATTTAGACAAAGCTAGCAGAGTCTCTGTAGAAA TGCTGGAAAAGAACGTGAAGCAGATGGGAAGGCAGCTTCAACAGCTTGAGAAGAATTTGGAAACCTTTCC CCCTCCTGAGGACTTGCATGACAAGTTTGTGATAAAGATGTCCAGCTTCGTTATCAGTGCGAACGAGCAG TATGAAAAACTCTCCACACTACTGGGCAGCATGACACAATTGTACCAGAGTATAATGGGCTACTATGCTG TCGACATGAAGAAGGTTTCCGTGGAAGAGTTTTTTAATGATCTGAACAACTTCAGAACTTCATTTATGCT AGCATTAAAGGAAAACATCAAAAAACGAGAAGCAGCAGAAAAGGAGAAACGTGCCAGGATAGCGAAAGAG CGAGCAGAGAAAGAGCGACTTGAACGCCAGCAAGAGAAAAAGCGCTTACTAGAAATGAAAACTGAGGGAG ATGAGACAGGAGTGATGGATAGTCTGCTGGAGGCCTTGCAGTCAGGGGCTGCCTTCCGCGACAGAAGAAA AAGGACACCAAAGCTGAAAGATATTCGGCAGAGTCTCAGCCCGATGTCTCAGAGGCCTGTTCTCAAAGTT TGTAACCATGAAAATCAGAAAATGCAGTTGACAGAAGGGTCACGTCCACACCACAGTATCAATTGCAACT CCACCAGGACTCCAGTCGCCAAGGAGCTTAATTATAATCTAGACACTCATGCGTCTACAGGGAGGATCAA GGCAGTTGAGAAGGAAGCCTGTAATGCAGAAAGCAACAGAAAAAAGGAAATGGAACTTCTTGGCTCTGTT GCTAAAAGCGAATCAGTTCCTGAAGTTGAAGCCCTGCTGGCAAGATTACGAGCTTTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | BC028920 |
Insert Size | 2790 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC028920, AAH28920 |
RefSeq Size | 3770 bp |
RefSeq ORF | 2789 bp |
Locus ID | 56419 |
Gene Summary | Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers (PubMed:10678165, PubMed:23558171). Required for cytokinesis, stress fiber formation and transcriptional activation of the serum response factor (PubMed:10678165, PubMed:23558171). Binds to GTP-bound form of Rho and to profilin: acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization (PubMed:10678165). DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (PubMed:10678165). Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity (PubMed:23558171).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG211218 | Diap3 (tGFP-tagged) - Mouse diaphanous homolog 3 (Drosophila) (cDNA clone MGC:30844 IMAGE:4008236) |
CNY 5,890.00 |
|
MR211218 | Diap3 (Myc-DDK-tagged) - Mouse diaphanous homolog 3 (Drosophila) (cDNA clone MGC:30844 IMAGE:4008236) |
CNY 6,808.00 |
|
MR211218L3 | Lenti ORF clone of Diap3 (Myc-DDK-tagged) - Mouse diaphanous homolog 3 (Drosophila) (cDNA clone MGC:30844 IMAGE:4008236) |
CNY 7,220.00 |
|
MR211218L4 | Lenti ORF clone of Diap3 (mGFP-tagged) - Mouse diaphanous homolog 3 (Drosophila) (cDNA clone MGC:30844 IMAGE:4008236) |
CNY 7,220.00 |