Palb2 (BC066140) Mouse Untagged Clone

CAT#: MC220917

Palb2 (untagged) - Mouse partner and localizer of BRCA2 (cDNA clone MGC:76443 IMAGE:30459312), (10ug)



  "BC066140" in other vectors (4)

CNY 9,120.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 4732427B05
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>BC066140
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGAGCTTTCCGGGAAGCCCCTCAGCTATGCGGAGAAGGAAAAGTTGAAGGAAAAGCTAGCATTTT
TGAAAAAGGAATACAGCAGGACACTTGCTCGACTTCAGCGTGCCAAAAGAGCTGAGAAGGCTAAGAACTC
TAAGAAAGCCATAGAAGATGGTGTGCCCCAGCCAGAAGCTTCCTCACAGCTAAGCCACTCTGAATCTATA
AATAAAGGCTTTCCTTGTGACACATTGCAAAGCAACCATCTGGATGAGGAGACTGGAGAAAACATCTCTC
AGATACTTGATGTTGAACCTCAATCCTTTAACTGTAAACAAGGCAAAGAAGTATTACATACACCAAGAGC
AGGTGACATTCAAGGACAACTTTTGCATAGCACCAGCAGCCCTGATGGCAAGAAAGAGCAGAACACGCTT
CCGGGGACAACAAAGACGCCGTGGGAGAAGTCATCTGTTTCACAGGAGAAGGAAGATTATTTTGACACTA
ATTCTCTGGCGCTCCTTGACTTTGAGTTACCTGATGAAGACTTTGGGCTTCTTAAACTTGAAAAATTGAA
GTCCTGCTCGGAAAAACTGATTGAGTCTCCTGACTCAAAAAACTGTGGTGAGAGGCTTCCTAGAGAAGGA
AACCATGCTGCTCTGGAGGAACTGCAAAGAGATTCGGAGACGGAGGGCTTGGAAGAGGAGCTCACTGTTC
CACCAGGAGAAGCGTACCGTCCAGGGCCAACCCTGAGAAGGCAGCCAGGGAGCAAGGACCTTTCTTCATC
CATAGTGCTTTTCACTCCTGCAGACACTGCTGCGCCTAACGACAGTGGCAGGCCTCCTCCCTCCCTGTGC
TCACCTGCTTTCCCCATCTTAGGCATGACTCCAGCTCTTGGCTCCCAAGCAGCCGGTGAGACTCTATCTA
CTGAAGCTGCACAGCCTTGCTCTACATCCCAACCTCCTCTCTTGGGAGACACAAACAGTCTTGTCAATAA
CAGTAAACAGTGCAACAGTTCAGCCTGCTCACCAAAACCGGACACCAACCTGCAAGCGTCAGGTAGGCAA
GGACAACCTGCCTGTGACAGTGACTCTGGCCCCCAAGCAACACCTCTACCTGTTGAGTCATTCACTTTCA
GAGAAAATCAGCTTTGTGGAAATGCATGCCTCGAGTTACATGAACATTCCACTGAACAGACTGAAACTGC
AGATCGCCCTGCTTGTGACAATCTAAACCCAGGAAACCTACAGTTGGTTTCAGAGTTAAAGAATCCTTCC
AGTTCCTGCTCTGTGGATGTGAGCGCCATGTGGTGGGAAAGAGCTGGTGCTAAGGAGCCATGTATCGTAA
CTGCTTGTGAAGATGTAGTTTCTCTTTGGAAACCCTTGAATTCTCTGCAGTGGGAGAAAGTTCATACCTG
GCACTTCACAGAGGTTCCAGTATTACAAATAGTTCCAGTGCCTGATGTTTACAATCTTATATGTGTAGCT
TTGGGAAGTTTGGAAATCAGAGAAATCAGGGCGTTGCTGTGCTCTTCTGGTGATGACAGTGAAAAGCAAG
TCCTCCTGAAATCTGGAGATATAAAAGCTATGCTTGGCCTGACAAAGCGGAGGCTAGTTAGTAGCACTGG
GACCTTTTGCAATCAACAAATACAAATCATGACATTTGCTGACGACGGAAGCAGCAAAGATGAGCAGCTT
TTGATGCCTCCTGATGAGACTGTACTGACTTTTGCTGAAGTCCAAGGGACGCAGGAAGCTCTGCTTGGTA
CTACCACTGTGAACAGCATTGTGATCTGGAATTTAAAAACCGGCCAGCTCCTGAAGAAGATGCACATTGA
TGACTCCTACCAGGCTTCGGTCTGTCACGGAGCCTATTCTGAGAAGGGGCTCCTGTTTGTTGTTGTGAGT
CAACCTTGTGCCAAAGAGAGCCAGGCCCTTGGAAGCCCTGTGTTCCAGCTGCTGGTGATTAACCCTAAGA
CGGCCCAGAGTGTGGGTGTTCTGCTGTGTTCCCTTCCTCAGGGGCAGGCTGGAAGGTTCCTGGAAGGGGA
TGTGAAAGATCATGTCGCAGCAGCAGTCCTGACTTCTGGGACAATTGCCATTTGGGATTTGCTTCTGGGT
CACTGCACGGCTCTCCTCCCACCTGTCTCTGACCAGAGTTGGTCTTTGGTTAAATGGTCGGGTACAGATT
CTCACTTGCTAGCTGGACAAAAGGATGGAAATATATTTATATACCGATACTTTTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Restriction Sites SgfI-MluI     
ACCN BC066140
Insert Size 2226 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC066140, AAH66140
RefSeq Size 2568 bp
RefSeq ORF 2225 bp
Locus ID 233826
Gene Summary Plays a critical role in homologous recombination repair (HRR) through its ability to recruit BRCA2 and RAD51 to DNA breaks. Strongly stimulates the DNA strand-invasion activity of RAD51, stabilizes the nucleoprotein filament against a disruptive BRC3-BRC4 polypeptide and helps RAD51 to overcome the suppressive effect of replication protein A (RPA). Functionally cooperates with RAD51AP1 in promoting of D-loop formation by RAD51. Serves as the molecular scaffold in the formation of the BRCA1-PALB2-BRCA2 complex which is essential for homologous recombination. Via its WD repeats is proposed to scaffold a HR complex containing RAD51C and BRCA2 which is thought to play a role in HR-mediated DNA repair. Essential partner of BRCA2 that promotes the localization and stability of BRCA2. Also enables its recombinational repair and checkpoint functions of BRCA2. May act by promoting stable association of BRCA2 with nuclear structures, allowing BRCA2 to escape the effects of proteasome-mediated degradation. Binds DNA with high affinity for D loop, which comprises single-stranded, double-stranded and branched DNA structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with BRCA2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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