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Top1 (NM_009408) Mouse Untagged Clone
CAT＃: MC221438
Top1 (untagged) - Mouse topoisomerase (DNA) I (Top1), (10ug)
CNY 9,880.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AI467334; D130064I21Rik; Top-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC221438 representing NM_009408
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGGGGACCATCTCCACAACGATTCCCAGATCGAAGCGGATTTCCGATTGAATGATTCTCACAAAC ACAAAGATAAACACAAAGATCGAGAACACCGGCATAAGGAGCACAAGAAGGATAAGGATAAGGACCGGGA AAAGTCTAAGCATAGCAACAGTGAACATAAAGATTCTGAAAAGAAACACAAAGAGAAAGAGAAAACCAAA CACAAAGATGGCAGCTCAGAAAAACATAAAGACAAACATAAAGACAGAGACAAGGAAAGACGAAAGGAGG AAAAGATTAGAGCTGCTGGGGATGCAAAAATAAAGAAGGAAAAGGAAAATGGCTTCTCTAGTCCGCCACG AATTAAAGATGAACCTGAGGATGATGGCTATTTTGCTCCTCCAAAAGAGGATATTAAGCCATTGAAGAGG CTTCGAGATGAAGATGATGCTGATTATAAACCTAAGAAAATTAAGACAGAAGATATCAAGAAGGAGAAGA AAAGAAAATCAGAAGAGGAAGAGGATGGTAAACTTAAAAAGCCCAAGAATAAAGATAAAGATAAAAAAGT TGCTGAGCCAGATAATAAGAAAAAGAAGCCGAAAAAGGAAGAGGAACAGAAGTGGAAATGGTGGGAAGAA GAACGTTATCCAGAAGGCATCAAATGGAAATTCCTAGAGCATAAAGGGCCTGTCTTCGCTCCACCATATG AGCCTCTGCCAGAGAGTGTCAAGTTTTACTATGATGGTAAAGTTATGAAGCTGAGTCCTAAAGCAGAAGA AGTAGCTACATTCTTTGCAAAAATGCTTGACCACGAATATACTACTAAGGAAATATTCAGGAAAAATTTC TTTAAAGATTGGAGAAAGGAAATGACTAATGACGAAAAAAATACGATTACCAACCTAAGCAAATGCGACT TTACACAGATGAGCCAGTATTTCAAAGCGCAGTCAGAAGCTCGGAAACAGATGAGCAAGGAAGAAAAATT GAAAATCAAAGAAGAAAATGAAAAGTTATTGAAAGAATACGGCTTTTGTGTAATGGATAATCATAGAGAG CGGATTGCCAACTTCAAGATAGAGCCTCCGGGGCTTTTCCGAGGCCGAGGGAACCACCCCAAGATGGGTA TGCTGAAGAGAAGGATCATGCCTGAGGACATCATCATCAACTGTAGCAAAGACGCAAAGGTTCCTTCTCC CCCTCCTGGACATAAATGGAAGGAAGTCCGACATGATAACAAGGTTACTTGGCTGGTCTCCTGGACAGAG AATATCCAAGGTTCTATCAAATATATCATGCTGAATCCCAGTTCACGAATCAAGGGTGAGAAAGACTGGC AGAAGTATGAGACTGCGCGGCGTCTGAAGAAGTGTGTGGACAAGATCCGAAATCAGTATCGGGAAGACTG GAAGTCCAAAGAGATGAAAGTTCGTCAGAGAGCTGTAGCACTGTACTTCATTGATAAGCTTGCTCTGAGA GCAGGCAATGAGAAGGAAGAAGGAGAGACGGCAGACACTGTGGGCTGCTGTTCACTTAGAGTGGAACACA TCAATTTACACCCAGAATTGGATGGTCAGGAGTATGTGGTGGAATTTGACTTTCCTGGGAAGGACTCAAT CAGATACTATAACAAAGTCCCAGTTGAGAAACGAGTTTTTAAAAACTTACAACTGTTTATGGAGAACAAG CAGCCTGAGGACGATCTTTTTGATCGACTTAATACTGGTATTCTAAATAAACATCTTCAGGATCTCATGG AGGGCTTGACTGCCAAGGTGTTCCGTACCTACAATGCCTCCATCACACTACAGCAGCAGCTTAAAGAGCT CACAGCCCCTGATGAGAATGTACCAGCGAAGATTCTATCTTATAACCGTGCCAATCGAGCTGTTGCAATT CTTTGTAACCACCAGAGGGCGCCACCAAAGACCTTTGAGAAGTCAATGATGAACTTGCAGTCTAAGATTG ATGCCAAGAAAGATCAGTTAGCAGATGCTCGAAGGGACCTGAAAAGTGCTAAGGCTGATGCCAAAGTCAT GAAGGATGCAAAGACCAAGAAGGTAGTAGAGTCAAAAAAGAAGGCTGTACAGAGACTAGAAGAGCAGTTG ATGAAGCTGGAAGTTCAAGCCACAGATCGAGAGGAGAACAAACAAATTGCCTTGGGGACCTCCAAACTCA ATTATCTGGACCCTAGGATCACAGTGGCTTGGTGCAAAAAGTGGGGGGTCCCAATTGAGAAGATTTACAA CAAAACCCAGAGGGAGAAGTTTGCTTGGGCCATTGATATGACTGATGAGGACTACGAGTTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_009408 |
| Insert Size | 2304 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_009408.2, NP_033434.2 |
| RefSeq Size | 3859 bp |
| RefSeq ORF | 2304 bp |
| Locus ID | 21969 |
| UniProt ID | Q04750 |
| Gene Summary | Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG218547 | Top1 (tGFP-tagged) - Mouse topoisomerase (DNA) I (Top1), (10ug). |
CNY 10,024.00 |
|
| MR218547 | Top1 (Myc-DDK-tagged) - Mouse topoisomerase (DNA) I (Top1). Note: ORF is codon optimized |
CNY 8,424.00 |
|
| MR218547L3 | Lenti ORF clone of Top1 (Myc-DDK-tagged) - Mouse topoisomerase (DNA) I (Top1). Note: ORF is codon optimized |
CNY 10,824.00 |
|
| MR218547L4 | Lenti ORF clone of Top1 (mGFP-tagged) - Mouse topoisomerase (DNA) I (Top1). Note: ORF is codon optimized |
CNY 7,700.00 |
