United States
Germany
Japan
United Kingdom
China
Top1 (NM_009408) Mouse Tagged ORF Clone Lentiviral Particle
CAT＃: MR218547L3V
- LentiORF®
-
Lenti ORF particles, Top1 (Myc-DDK-tagged) - Mouse topoisomerase (DNA) I (Top1), 200ul, >10^7 TU/mL. Note: ORF is codon optimized
Need custom lentivirus service?
Get a free quote
CNY 12,540.00
货期*
详询
规格
Product images
经常一起买 (2)
Specifications
| Product Data | |
| Product Name | Top1 (NM_009408) Mouse Tagged ORF Clone Lentiviral Particle |
| Synonyms | AI467334; D130064I21Rik; Top-1 |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| ACCN | NM_009408 |
| ORF Size | 2301 bp |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR218547).
|
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Reference Data | |
| RefSeq | NM_009408.2, NP_033434.2 |
| RefSeq Size | 3859 bp |
| RefSeq ORF | 2304 bp |
| Locus ID | 21969 |
| Gene Summary | Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
You may also need
Customer
Reviews
Loading...
