Sp1 (NM_013672) Mouse Untagged Clone
CAT#: MC221564
Sp1 (untagged) - Mouse trans-acting transcription factor 1 (Sp1), (10ug)
CNY 5,728.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 1110003E12Rik; AA450830; AI845540; Sp1-1 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC221564 representing NM_013672
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGACCAAGATCACTCCATGGATGAAGTGACAGCTGTGGTGAAGATTGAAAAAGATGTTGGTGGCA ATAATGGGGGTAGCGGCAATGGTGGCGGTGCCGCCTTTTCTCAGACTCGAAGCAGCAGCACAGGCAGTAG CAGCAGCAGTGGTGGCGGAGGAGGGCAGGAATCCCAGCCATCTCCTTTGGCTCTGCTGGCAGCAACCTGC AGCAGAATTGAGTCACCCAATGAGAACAGCAACAACTCCCAGGGTCCGAGTCAGTCAGGGGGCACAGGTG AACTTGACCTCACAGCCGCACAACTTTCACAGGGTGCCAATGGCTGGCAGATCATCTCTTCCTCCTCTGG GGCTACCCCTACCTCAAAGGAACAGAGTGGCAACAGTACCAATGGCAGCGAGTCTTCCAAGAACCGCACA GTCTCTGGTGGGCAGTATGTTGTGGCTGCTACCCCCAACTTACAGAACCAGCAAGTTCTGACAGGTCTCC CTGGAGTAATGCCTAATATTCAGTATCAAGTAATCCCACAGTTCCAGACTGTTGATGGGCAGCAGCTGCA GTTTGCTGCCACTGGGGCCCAAGTGCAGCAGGATGGTTCTGGTCAAATACAGATCATACCAGGTGCAAAC CAACAGATCATCCCAAATAGAGGAAGTGGGGGCAACATTATTGCTGCTATGCCAAATCTACTCCAGCAGG CTGTCCCCCTTCAAGGCCTTGCTAATAATGTGCTCTCAGGACAGACTCAGTATGTGACCAATGTACCAGT GGCCCTGAATGGGAACATCACCTTGCTACCTGTCAACAGCGTTTCTGCAGCTACCCTGACTCCCAGCTCT CAGGCAGGCACTATCAGCAGCTCTGGATCCCAGGAGAGCAGCTCACAGCCTGTCACCTCAGGGACTGCCA TCAGTTCTGCCAGCTTGGTGTCATCACAAGCTAGTTCCAGCTCCTTTTTTACCAATGCCAATAGTTATTC AACAACTACTACCACCAGCAACATGGGAATTATGAACTTTACCAGCAGTGGCTCATCAGGGACTAGTTCT CAAGGCCAGACGCCCCAGAGGGTTGGTGGGCTACAAGGGTCTGATTCTCTGAACATCCAGCAGAACCAGA CATCAGGAGGCTCGCTGCAAGGAAGTCAGCAGAAAGAGGGAGAGCAAAGTCAGCAGACACAGCAACAACA AATCCTTATTCAGCCTCAGCTAGTTCAAGGAGGACAAGCTCTTCAGGCCCTTCAAGCAGCACCATTGTCC GGACAGACCTTCACAACTCAAGCTATTTCCCAGGAAACCCTTCAGAACCTCCAGCTTCAGGCTGTTCAAA ACTCTGGTCCCATCATCATTCGGACACCAACCGTGGGGCCCAATGGACAGGTCAGTTGGCAGACCCTTCA GCTGCAGAATCTTCAAGTTCAGAACCCACAAGCCCAGACAATCACCTTGGCCCCTATGCAGGGTGTTTCC TTGGGGCAGACTAGCAGCAGTAATACCACCCTAACACCCATTGCCTCAGCTGCCTCCATTCCTGCTGGCA CAGTCACTGTGAATGCTGCTCAACTCTCCTCCATGCCAGGCCTCCAGACCATTAACCTCAGTGCATTGGG TACTTCAGGGATCCAGGTGCACCAGCTTCCAGGCCTGCCTTTGGCTATAGCAAACACCCCAGGTGATCAT GGAACTCAACTTGGTCTTCATGGATCTGGTGGTGATGGGATACATGATGAGACAGCAGGTGGAGAAGGAG AGAATAGCTCTGATCTCCAACCCCAAGCTGGACGCAGGACTCGTCGGGAAGCATGTACATGCCCCTATTG CAAAGACAGTGAGGGAAGAGCCTCAGGAGATCCTGGCAAAAAGAAACAGCACATTTGTCACATCCAAGGA TGCGGCAAAGTATATGGCAAGACCTCACATCTCCGAGCACACTTGCGCTGGCATACAGGGGAGAGGCCAT TCATGTGTAATTGGTCATATTGTGGGAAGCGCTTTACACGTTCGGACGAGCTTCAGAGACATAAACGTAC ACATACAGGAGAGAAGAAATTTGCCTGCCCTGAGTGCCCTAAGCGTTTCATGAGGAGTGATCACCTGTCA AAGCATATCAAGACTCACCAGAACAAGAAGGGAGGCCCAGGTGTAGCCCTGAGTGTGGGCACATTGCCCC TGGACAGTGGGGCAGGTTCAGAAGGCACTGCCACTCCTTCAGCCCTTATTACCACCAATATGGTAGCCAT GGAGGCCATCTGTCCAGAGGGTATTGCCCGTCTTGCCAACAGTGGCATCAACGTCATGCAGGTGACAGAG CTGCAGTCCATTAATATCAGTGGCAATGGTTTCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI |
ACCN | NM_013672 |
Insert Size | 2346 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_013672.2, NP_038700.2 |
RefSeq Size | 7818 bp |
RefSeq ORF | 2346 bp |
Locus ID | 20683 |
UniProt ID | O89090 |
Gene Summary | Transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. Binds with high affinity to GC-rich motifs and regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Highly regulated by post-translational modifications (phosphorylations, sumoylation, proteolytic cleavage, glycosylation and acetylation). Binds also the PDGFR-alpha G-box promoter. May have a role in modulating the cellular response to DNA damage. Implicated in chromatin remodeling. Plays a role in the recruitment of SMARCA4/BRG1 on the c-FOS promoter Plays an essential role in the regulation of FE65 gene expression (By similarity). Positively regulates the transcription of the core clock component ARNTL/BMAL1 (PubMed:24030830). Plays a role in protecting cells against oxidative stress following brain injury by regulating the expression of RNF112 (PubMed:27918959).[UniProtKB/Swiss-Prot Function] |
Documents
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SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG225692 | Sp1 (tGFP-tagged) - Mouse trans-acting transcription factor 1 (Sp1), (10ug) |
CNY 7,320.00 |
|
MR225692 | Sp1 (Myc-DDK-tagged) - Mouse trans-acting transcription factor 1 (Sp1) |
CNY 5,720.00 |
|
MR225692L3 | Lenti ORF clone of Sp1 (Myc-DDK-tagged) - Mouse trans-acting transcription factor 1 (Sp1) |
CNY 7,700.00 |
|
MR225692L4 | Lenti ORF clone of Sp1 (mGFP-tagged) - Mouse trans-acting transcription factor 1 (Sp1) |
CNY 7,700.00 |