Vps51 (NM_001081041) Mouse Untagged Clone

CAT#: MC221574

Vps51 (untagged) - Mouse RIKEN cDNA 1110014N23 gene (1110014N23Rik), (10ug)



  "NM_001081041" in other vectors (4)

CNY 10,070.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 1110014N23Rik; 3110057M17Rik; AI837850; Ffr
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC221574 representing NM_001081041
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCTGCGGCAGCTGTGGGGCCTGGCCTAGGGTCCGGGCCTGGAGACTCCCCAGAAGGTCCCGAGG
CGGACGCTCCGGAGCGTCGGCGGAAGGCTCACGGGATGCTGAAGCTTTACTACGGCCTCTCGGAGGGGGA
GGTGGCGGGGCATCCCGCAGGGCCTGACCCTCTGGACCCAACCGATCTCAATGGGGCGCACTTCGACCCG
GAAGTTTATCTTGACAAGCTGCGTAGAGAATGTCCTCTGGCCCAGCTGATGGACAGTGAGACGGACATGG
TACGGCAGATCCGGGCTCTAGACAGCGACATGCAGACCCTGGTCTATGAGAACTACAACAAGTTCATCTC
AGCTACAGACACCATTCGAAAGATGAAGAATGACTTCCGGAAGATGGAAGATGAGATGGACCGGCTGGCC
ACCAACATGGCAGTGATCACCAACTTCAGCGCACGGATCAGCGCCACGCTACAGGACCGACACGAGCGCA
TCACCAAGCTAGCAGGGGTACATGCACTACTGCGGAAGCTGCAGTTCCTTTTTGAGCTGCCTTCGCGCCT
CACCAAGTGTGTGGAGCTGGGTGCCTATGGGCAGGCAGTGCGCTATCAGGGTCGTGCCAGGGCCGTGCTG
CAGCAGTACCAGCACCTGCCCTCCTTCCGTGCCATACAGGATGACTGCCAGGTCATCACAGCTCGCTTGG
CCCAGCAGCTGCGGCAGCGCTTCAGGGAAGGCTGCTCAGGTGCCCCGGAGCAGGCCGAGTGTGTGGAGCT
GCTGCTGGCCCTGGGCGAGCCAGCTGAGGAGCTGTGCGAGGAGTTCCTGGCGCATGCGCGGGGACGGCTG
GAGGAAGAACTGAGCAGCCTGGAGGCCGAACTGGGGCCCTCCCCTCCAGCACCTGATGTGTTAGAGTTCA
CCGACCGTGGTGGCAATGGTTTTGTAGGTGGCCTCTGCCAGGTGGCAGCAGCCTACCAAGAGCTGTTTGC
TGCTCAGGGCCCTGCTGGTGCGGAGAAGCTGGCAGCCTTTGCGCAGGAGCTGGGCGGCCGCTACTTTGCA
CTGGTTGAGCGGCGGCTGGCACAGGAGCAGGGTGGTAGTGACAACTCACTGCTGGTGCGGGCACTAGATC
GCTTCCACCGGCGCCTGCGAGCACCTGGGGCCCTGCTGGCCGCTGCTGGGCTTTCAGAGTCAGCCACAGA
GATTGTGGAGCGAGTGGCCCGGGAGCGCCTGAGCCACCATCTGCAGGGTCTGAAGGCTGCCTTCCTGAGC
TCCCTGACGGATGTTCGCCAGGCCCTGGCAGCACCTCGCCTAGCGGGGAAGGAGGGCCCCAGCCTGGCTG
AGTTGCTGGCCAATGTGGCCAGCTCCATCCTAAGCCACATCAAGACGTCACTGGCTTCTGTGCATCTCTT
TACCGCCAAGGAGGTGTCCTTCTCCAATAAGCCCTACTTCAGGGGCGAGTTCTGCAGCCAGGGAGTTCGT
GAGGGCCTCATCGTGGGTTTCATCCGCTCCATGTGCCAGACAGCCCAGAGCTTCTGTGACAGCCCTGGAG
AAAAGGGAGGTGCCACGCCGCCTGCCCTGCTCCTGCTGCTCTCTCGCCTCTGCCTGGATTATGAGACAGC
CACCATCTCCTACATCCTCACACTCACTGATGAACAGTTTCTGGTGCAGGACCAGTCTCCCGTTACACCC
GTGAGCACACTGTGTGCAGAGGCCAGAGAGACAGCACGGCGGCTGCTGACTCACTATGTGAAGGTGCAAG
GCCTGGTCATATCCCAGATGCTACGTAAGAGTGTGGAGACCCGGGACTGGCTCAGTACTCTGGAGCCTAG
GAACGTGCGTGCTGTCATGAAGCGTGTGGTGGAGGACACTACAGCTATTGATGTCCAGGTGGGGCTCCTG
TATGAAGAGGGTGTCCGGAAGGCCCAGAGCAGTGACTCAAGCAAAAGGACTTTCTCTGTGTACAGCAGCT
CCCGGCAGCAGGGCCGCTATGCACCCAGCTACACACCCAGTGCTCCCATGGACACAAACCTCCTGAGCAA
TATTCAGAAGCTGTTCTCTGAACGGATCGATGTGTTCAGCCCCGTGGAATTCAACAAGGTGTCGGTGCTG
ACTGGCATCATTAAGATCAGCTTGAAGACACTACTGGAGTGTGTGCGCCTACGCACCTTTGGGCGCTTTG
GGCTGCAGCAGGTGCAGGTGGACTGCCACTTCCTGCAGCTTTACTTGTGGCGCTTTGTGGCTGATGAGGA
GCTAGTGCACCTGCTGCTGGACGAAGTAGTAGCCTCAGCTGCTCTGCGCTGTCCCGACCCAGTGCCCATG
GAACCTAGTGTTGTCGAGGTCATCTGCGAGCGCGGCTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001081041
Insert Size 2349 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001081041.1, NP_001074510.1
RefSeq Size 2631 bp
RefSeq ORF 2349 bp
Locus ID 68505
UniProt ID Q3UVL4
Gene Summary Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of protein retrieval from endosomes to the TGN, acid hydrolase sorting, lysosome function, endosomal cholesterol traffic and autophagy. VPS51 participates in retrograde transport of acid hydrolase receptors, likely by promoting tethering and SNARE-dependent fusion of endosome-derived carriers to the TGN. Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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