Smo (NM_176996) Mouse Untagged Clone

CAT#: MC221670

Smo (untagged) - Mouse smoothened homolog (Drosophila) (Smo), (10ug)



  "NM_176996" in other vectors (4)

CNY 8,720.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms bnb; E130215L21Rik; Smoh; smoothened
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC221670 representing NM_176996
Red=Cloning site Blue=ORF

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGCTGGCCGCCCCGTGCGTGGGCCCGAGCTGGCGCCCCGGAGGCTGCTGCAGTTGCTTCTGCTGG
TACTGCTGGGGGGCCCGGGCCGGGGGGCGGCCTTGAGCGGGAACGTGACCGGGCCTGGGCCTCACAGCGC
CAGCGGGAGCTCGAGGAGGGACGTGCCGGTGACCAGCCCTCCGCCGCCGCTGCTGAGCCACTGCGGCCGG
GCCGCCCACTGCGAGCCTTTGCGCTACAACGTGTGCCTGGGCTCGGCGCTGCCCTACGGAGCCACCACCA
CGCTGCTGGCTGGGGACTCGGACTCGCAGGAGGAAGCGCACGGCAAGCTCGTGCTCTGGTCCGGCCTCCG
GAATGCCCCCCGCTGCTGGGCAGTGATCCAGCCCCTGCTGTGTGCTGTCTACATGCCCAAGTGTGAGAAT
GACCGAGTGGAGTTGCCCAGCCGTACCCTCTGCCAGGCCACCCGAGGCCCCTGTGCCATTGTGGAGCGGG
AGCGAGGGTGGCCTGACTTTCTGCGTTGCACACCGGACCACTTCCCTGAAGGCTGCCCAAACGAGGTACA
AAACATCAAGTTCAACAGCTCAGGCCAATGTGAAGCACCCTTGGTGCGAACAGACAACCCCAAGAGCTGG
TATGAGGACGTGGAGGGCTGTGGGATTCAGTGTCAGAACCCGCTGTTCACCGAGGCCGAGCACCAGGACA
TGCACAGCTACATCGCAGCCTTCGGGGCGGTCACTGGTCTCTGCACGCTCTTCACACTGGCCACCTTTGT
GGCTGACTGGCGGAACTCCAATCGCTACCCTGCGGTTATTCTCTTCTATGTCAATGCGTGTTTCTTCGTG
GGCAGCATTGGCTGGCTGGCCCAGTTCATGGATGGTGCCCGCCGAGAGATTGTTTGCCGAGCAGATGGCA
CCATGAGATTTGGGGAGCCCACCTCCAGTGAGACCCTGTCCTGTGTCATCATCTTTGTCATTGTGTACTA
TGCCTTGATGGCTGGAGTAGTCTGGTTCGTGGTCCTCACCTATGCCTGGCACACCTCCTTCAAAGCCCTG
GGCACCACCTACCAGCCTCTCTCGGGCAAGACATCCTATTTCCACCTGCTCACGTGGTCACTCCCCTTTG
TCCTCACGGTGGCAATCCTGGCTGTGGCTCAGGTAGATGGAGACTCCGTGAGTGGCATCTGTTTTGTAGG
CTACAAGAACTATCGGTACCGTGCTGGCTTTGTCCTGGCCCCAATTGGCCTGGTGCTTATTGTGGGAGGC
TACTTCCTCATCAGAGGGGTCATGACTCTGTTCTCCATCAAGAGCAACCACCCTGGGCTTCTGAGTGAGA
AGGCAGCCAGCAAGATCAACGAGACCATGCTGCGCCTGGGCATTTTTGGCTTCCTGGCCTTTGGCTTTGT
GCTCATCACCTTCAGCTGCCACTTCTATGACTTCTTCAACCAGGCTGAGTGGGAGCGTAGCTTCCGGGAC
TATGTGCTATGCCAAGCCAACGTGACCATCGGGCTGCCTACCAAGAAGCCCATTCCTGACTGTGAGATCA
AGAATCGGCCCAGCCTCCTGGTGGAGAAGATCAATCTATTTGCCATGTTTGGCACTGGCATTGCCATGAG
CACCTGGGTCTGGACCAAGGCCACCCTGCTCATCTGGAGGCGCACCTGGTGCAGGTTGACTGGGCACAGT
GATGATGAGCCCAAGAGAATCAAGAAGAGCAAGATGATCGCCAAGGCCTTCTCTAAGCGGCGTGAGCTGC
TGCAGAACCCGGGCCAGGAGCTCTCCTTCAGCATGCACACTGTCTCCCATGATGGACCTGTTGCGGGTTT
GGCTTTTGACCTCAATGAACCCTCAGCTGACGTCTCCTCTGCCTGGGCTCAGCATGTCACCAAGATGGTG
GCTCGGAGAGGAGCCATATTGCCCCAGGATGTGTCCGTTACCCCTGTGGCAACTCCAGTGCCACCAGAAG
AACAAGCCAACATGTGGCTGGTTGAGGCAGAGATCTCCCCAGAGTTAGAGAAGCGTTTGGGCCGGAAGAA
AAAGCGGAGGAAGAGGAAGAAGGAGGTGTGCCCCTTGAGGCCAGCCCCTGAGCTTCACCACTCTGCCCCT
GTTCCTGCCACCAGTGCAGTTCCTCGGCTGCCTCAGCTGCCTCGGCAGAAGTGCCTGGTAGCTGCAAACG
CCTGGGGAACAGGGGAGTCCTGCCGACAGGGAGCCTGGACTCTAGTCTCCAACCCCTTCTGCCCAGAGCC
TAGTCCCCATCAAGATCCATTTCTCCCTGGTGCCTCAGCCCCCCGGGTCTGGGCTCAGGGCCGCCTCCAG
GGGCTGGGATCCATTCATTCCCGCACTAACCTAATGGAGGCTGAGATCTTGGATGCAGACTCGGACTTCT
GA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_176996
Insert Size 2382 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC096028, AAH96028
RefSeq Size 3542 bp
RefSeq ORF 2382 bp
Locus ID 319757
UniProt ID P56726
Gene Summary G protein-coupled receptor that probably associates with the patched protein (PTCH) to transduce the hedgehog's proteins signal. Binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO) (By similarity). Required for the accumulation of KIF7, GLI2 and GLI3 in the cilia. Interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation (PubMed:25644602).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...