Ift88 (NM_009376) Mouse Untagged Clone

CAT#: MC221866

Ift88 (untagged) - Mouse intraflagellar transport 88 homolog (Chlamydomonas) (Ift88), (10ug)



  "NM_009376" in other vectors (4)

CNY 9,072.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AW552028; flexo; fxo; orpk; polaris; Tg737; Tg737Rpw; TgN737Rpw; Ttc10
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC221866 representing NM_009376
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGGAAAATGTTCATCTGGCACCAGAAACAGATGAGGACGACCTTTACTCTGGTTTCAACGACTACA
ACCCAGCCTATGATACTGAGGAGTTGGAGAATGACACGGGTTTTCAGCAAGCAGTGAGAACCAGTCATGG
CAGAAGACCTCCCGTAACTGCTAAAATACCAAGCACAGCAGTCAGCAGACCCATAGCTACTGGATATGGG
TCCAAGACATCCCTGACATCATCAATGGGAAGACCGATGACAGGGACAATTCAGGATGGAGTTGCTCGAC
CGATGACAGCAGTGAGAGCAGCTGGCTTTTCCAAGGCAGCTTTGCGAGGCTCTGCATTTGACCCCCTTGG
TCAGTCCAGGGGCCCTGCTCCTCCTTTGGAAGCCAAAAATGAAGACAGTCCAGAAGAAAAAATTAGACAG
TTGGAGAAGAAAGTAAATGAGTTGGTGGAAGAAAGCTGTATTGCCAACAGTTGTGGAGACTTAAAACTGG
CCTTAGAGAAGGCAAAAGATGCAGGAAGAAAAGAGAGAGTCCTGGTGAGACAACGAGAGCAAGTCACAAG
TCCAGAAAATATCAACTTGGACTTAACCTACTCCGTTCTTTTCAACTTGGCCAGTCAGTATTCTGCTAAT
GAAATGTATGCAGAAGCACTGAACACATACCAAGTTATAGTGAAAAACAAGATGTTTAGCAATGCAGGAA
GACTGAAAGTGAATATGGGAAATATTTATTTAAAGCAGAGAAATTATTCCAAAGCCATTAAGTTCTACCG
AATGGCCTTAGATCAGATCCCAAGTGTCCATAAAGAAATGAGGATCAAAATAATGCAGAACATTGGAATC
ACATTTATAAAAACTGGTCAGTACTCAGATGCCATCAACTCATTTGAGCACATCATGAGCATGGCCCCGA
GCCTGAAGGCAGGCTTCAACCTCATCCTTAGTTGTTTTGCCATTGGAGATCGGGAGAAAATGAAGAAGGC
ATTCCAAAAATTAATTGCTGTTCCGTTAGAAATTGATGAAGATGATAAATATATCTCCCCAAGTGATGAT
CCCCATACTAACTTGCTGATTGAAGCTATAAAAAATGACCACCTTAGGCAAATGGAACGTGAAAGGAAAG
CCATGGCAGAAAAATACATCATGACAGCTGCAAAACTCATTGCTCCTGTGATCGAAGCGTCTTTTGCTGT
GGGTTATAACTGGTGTGTGGAAGTAGTGAAAGCCTCTCAGTATGTGGAGCTGGCCAACGACCTGGAGATT
AACAAAGCAATTACTTACTTGAGACAAAAGGACTTTAACCAAGCTGTAGACACATTGAAAATGTTTGAGA
AGAAGGACAGTAGAGTGAAGAGTGCAGCTGCGACCAACCTCTCGTTCCTGTATTATCTGGAAAACGAATT
TGCCCAAGCCAGCAGCTATGCAGATTTAGCTGTGAACTCGGATAGATATAACCCGTCAGCTCTCACTAAT
AAAGGGAACACAGTTTTTGCAAATGGTGATTATGAGAAGGCAGCTGAGTTCTATAAAGAGGCCCTGAGAA
ACGACTCTTCCTGTACTGAAGCACTGTATAACATTGGCCTCACCTATAAGAAGCTGAACCGTCTGGATGA
AGCCCTGGACTCCTTCCTGAAACTGCACGCGATCCTTCGGAACAGCGCTCAAGTTCTTTGCCAGATAGCA
AACATATATGAGTTAATGGAAGATCCCAATCAAGCTATTGAATGGCTGATGCAGTTGATCAGCGTTGTTC
CAACTGATTCCCAAGCTCTGTCTAAACTGGGAGAGTTATACGATAGTGAGGGAGACAAGTCCCAGGCATT
CCAATATTACTATGAGTCATACAGGTATTTCCCTTCTAACATTGAAGTCATTGAATGGCTTGGAGCTTAT
TACATTGATACACAGTTCTGCGAGAAAGCCATTCAGTACTTTGAAAGAGCTTCCCTTATACAACCCACAC
AAGTGAAGTGGCAGCTGATGGTAGCTAGCTGCTTTAGAAGAAGTGGTAACTACCAAAAGGCATTAGATAC
TTATAAAGAGATTCACAGGAAATTTCCGGAGAATGTTGAATGTTTGCGTTTCTTGGTTCGTCTCTGCACA
GATATTGGATTAAAAGAAGTTCAAGAATATGCCACAAAACTCAAGAGGTTGGAAAAAATGAAAGAAATGA
GGGAACAGCGCATAAAATCGGGCAGAGACAGCAGCGGGGGTTCTCGCAGCAAACGAGAGGGGAGTGCTGG
CAGTGACAGTGGCCAGAACAATAGTGCCAGTAGTAAGAGTGAACGACTGAGTGCCAAGCTCAGAGCTTTG
CCTGGGACAGATGAACCTTATGAAAGTAGCGGTAACAAAGAAATAGATGCCTCCTACGTGGATCCACTGG
GCCCTCAGATAGAAAGACCAAAAACTGCAGCCAAGAAAAGAATTGACGAGGATGATTTTGCTGATGAAGA
GTTAGGAGATGACTTGCTCCCAGAATAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_009376
Insert Size 2478 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_009376.2, NP_033402.2
RefSeq Size 3097 bp
RefSeq ORF 2478 bp
Locus ID 21821
UniProt ID Q61371
Gene Summary Involved in primary cilium biogenesis. Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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