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Ift88 (NM_009376) Mouse Tagged ORF Clone
CAT＃: MR210831
- TrueORF®
Ift88 (Myc-DDK-tagged) - Mouse intraflagellar transport 88 homolog (Chlamydomonas) (Ift88)
ORF Plasmid: tGFP
"NM_009376" in other vectors (4)
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CNY 9,064.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW552028; flexo; fxo; orpk; polaris; Tg737; Tg737Rpw; TgN737Rpw; Ttc10 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR210831 representing NM_009376
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGAAAATGTTCATCTGGCACCAGAAACAGATGAGGACGACCTTTACTCTGGTTTCAACGACTACA ACCCAGCCTATGATACTGAGGAGTTGGAGAATGACACGGGTTTTCAGCAAGCAGTGAGAACCAGTCATGG CAGAAGACCTCCCGTAACTGCTAAAATACCAAGCACAGCAGTCAGCAGACCCATAGCTACTGGATATGGG TCCAAGACATCCCTGACATCATCAATGGGAAGACCGATGACAGGGACAATTCAGGATGGAGTTGCTCGAC CGATGACAGCAGTGAGAGCAGCTGGCTTTTCCAAGGCAGCTTTGCGAGGCTCTGCATTTGACCCCCTTGG TCAGTCCAGGGGCCCTGCTCCTCCTTTGGAAGCCAAAAATGAAGACAGTCCAGAAGAAAAAATTAGACAG TTGGAGAAGAAAGTAAATGAGTTGGTGGAAGAAAGCTGTATTGCCAACAGTTGTGGAGACTTAAAACTGG CCTTAGAGAAGGCAAAAGATGCAGGAAGAAAAGAGAGAGTCCTGGTGAGACAACGAGAGCAAGTCACAAG TCCAGAAAATATCAACTTGGACTTAACCTACTCCGTTCTTTTCAACTTGGCCAGTCAGTATTCTGCTAAT GAAATGTATGCAGAAGCACTGAACACATACCAAGTTATAGTGAAAAACAAGATGTTTAGCAATGCAGGAA GACTGAAAGTGAATATGGGAAATATTTATTTAAAGCAGAGAAATTATTCCAAAGCCATTAAGTTCTACCG AATGGCCTTAGATCAGATCCCAAGTGTCCATAAAGAAATGAGGATCAAAATAATGCAGAACATTGGAATC ACATTTATAAAAACTGGTCAGTACTCAGATGCCATCAACTCATTTGAGCACATCATGAGCATGGCCCCGA GCCTGAAGGCAGGCTTCAACCTCATCCTTAGTTGTTTTGCCATTGGAGATCGGGAGAAAATGAAGAAGGC ATTCCAAAAATTAATTGCTGTTCCGTTAGAAATTGATGAAGATGATAAATATATCTCCCCAAGTGATGAT CCCCATACTAACTTGCTGATTGAAGCTATAAAAAATGACCACCTTAGGCAAATGGAACGTGAAAGGAAAG CCATGGCAGAAAAATACATCATGACAGCTGCAAAACTCATTGCTCCTGTGATCGAAGCGTCTTTTGCTGT GGGTTATAACTGGTGTGTGGAAGTAGTGAAAGCCTCTCAGTATGTGGAGCTGGCCAACGACCTGGAGATT AACAAAGCAATTACTTACTTGAGACAAAAGGACTTTAACCAAGCTGTAGACACATTGAAAATGTTTGAGA AGAAGGACAGTAGAGTGAAGAGTGCAGCTGCGACCAACCTCTCGTTCCTGTATTATCTGGAAAACGAATT TGCCCAAGCCAGCAGCTATGCAGATTTAGCTGTGAACTCGGATAGATATAACCCGTCAGCTCTCACTAAT AAAGGGAACACAGTTTTTGCAAATGGTGATTATGAGAAGGCAGCTGAGTTCTATAAAGAGGCCCTGAGAA ACGACTCTTCCTGTACTGAAGCACTGTATAACATTGGCCTCACCTATAAGAAGCTGAACCGTCTGGATGA AGCCCTGGACTCCTTCCTGAAACTGCACGCGATCCTTCGGAACAGCGCTCAAGTTCTTTGCCAGATAGCA AACATATATGAGTTAATGGAAGATCCCAATCAAGCTATTGAATGGCTGATGCAGTTGATCAGCGTTGTTC CAACTGATTCCCAAGCTCTGTCTAAACTGGGAGAGTTATACGATAGTGAGGGAGACAAGTCCCAGGCATT CCAATATTACTATGAGTCATACAGGTATTTCCCTTCTAACATTGAAGTCATTGAATGGCTTGGAGCTTAT TACATTGATACACAGTTCTGCGAGAAAGCCATTCAGTACTTTGAAAGAGCTTCCCTTATACAACCCACAC AAGTGAAGTGGCAGCTGATGGTAGCTAGCTGCTTTAGAAGAAGTGGTAACTACCAAAAGGCATTAGATAC TTATAAAGAGATTCACAGGAAATTTCCGGAGAATGTTGAATGTTTGCGTTTCTTGGTTCGTCTCTGCACA GATATTGGATTAAAAGAAGTTCAAGAATATGCCACAAAACTCAAGAGGTTGGAAAAAATGAAAGAAATGA GGGAACAGCGCATAAAATCGGGCAGAGACAGCAGCGGGGGTTCTCGCAGCAAACGAGAGGGGAGTGCTGG CAGTGACAGTGGCCAGAACAATAGTGCCAGTAGTAAGAGTGAACGACTGAGTGCCAAGCTCAGAGCTTTG CCTGGGACAGATGAACCTTATGAAAGTAGCGGTAACAAAGAAATAGATGCCTCCTACGTGGATCCACTGG GCCCTCAGATAGAAAGACCAAAAACTGCAGCCAAGAAAAGAATTGACGAGGATGATTTTGCTGATGAAGA GTTAGGAGATGACTTGCTCCCAGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR210831 representing NM_009376
Red=Cloning site Green=Tags(s) MMENVHLAPETDEDDLYSGFNDYNPAYDTEELENDTGFQQAVRTSHGRRPPVTAKIPSTAVSRPIATGYG SKTSLTSSMGRPMTGTIQDGVARPMTAVRAAGFSKAALRGSAFDPLGQSRGPAPPLEAKNEDSPEEKIRQ LEKKVNELVEESCIANSCGDLKLALEKAKDAGRKERVLVRQREQVTSPENINLDLTYSVLFNLASQYSAN EMYAEALNTYQVIVKNKMFSNAGRLKVNMGNIYLKQRNYSKAIKFYRMALDQIPSVHKEMRIKIMQNIGI TFIKTGQYSDAINSFEHIMSMAPSLKAGFNLILSCFAIGDREKMKKAFQKLIAVPLEIDEDDKYISPSDD PHTNLLIEAIKNDHLRQMERERKAMAEKYIMTAAKLIAPVIEASFAVGYNWCVEVVKASQYVELANDLEI NKAITYLRQKDFNQAVDTLKMFEKKDSRVKSAAATNLSFLYYLENEFAQASSYADLAVNSDRYNPSALTN KGNTVFANGDYEKAAEFYKEALRNDSSCTEALYNIGLTYKKLNRLDEALDSFLKLHAILRNSAQVLCQIA NIYELMEDPNQAIEWLMQLISVVPTDSQALSKLGELYDSEGDKSQAFQYYYESYRYFPSNIEVIEWLGAY YIDTQFCEKAIQYFERASLIQPTQVKWQLMVASCFRRSGNYQKALDTYKEIHRKFPENVECLRFLVRLCT DIGLKEVQEYATKLKRLEKMKEMREQRIKSGRDSSGGSRSKREGSAGSDSGQNNSASSKSERLSAKLRAL PGTDEPYESSGNKEIDASYVDPLGPQIERPKTAAKKRIDEDDFADEELGDDLLPE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009376 |
| ORF Size | 2475 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009376.2, NP_033402.2 |
| RefSeq Size | 3097 bp |
| RefSeq ORF | 2478 bp |
| Locus ID | 21821 |
| UniProt ID | Q61371 |
| MW | 93.6 kDa |
| Gene Summary | Involved in primary cilium biogenesis. Also involved in autophagy since it is required for trafficking of ATG16L and the expansion of the autophagic compartment.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221866 | Ift88 (untagged) - Mouse intraflagellar transport 88 homolog (Chlamydomonas) (Ift88), (10ug) |
CNY 9,072.00 |
|
| MG210831 | Ift88 (tGFP-tagged) - Mouse intraflagellar transport 88 homolog (Chlamydomonas) (Ift88) |
CNY 10,664.00 |
|
| MR210831L3 | Lenti ORF clone of Ift88 (Myc-DDK-tagged) - Mouse intraflagellar transport 88 homolog (Chlamydomonas) (Ift88) |
CNY 10,170.00 |
|
| MR210831L4 | Lenti ORF clone of Ift88 (mGFP-tagged) - Mouse intraflagellar transport 88 homolog (Chlamydomonas) (Ift88) |
CNY 10,170.00 |
