Hif1a (NM_010431) Mouse Untagged Clone

CAT#: MC221924

Hif1a (untagged) - Mouse hypoxia inducible factor 1, alpha subunit (Hif1a), (10ug)



  "NM_010431" in other vectors (6)

CNY 9,192.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AA959795; bHLHe7; bHLHe78; HIF-1; HIF-1-alpha; HIF1; HIF1-alpha; HIF1alpha; MO; MOP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC221924 representing NM_010431
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGGCGCCGGCGGCGAGAACGAGAAGAAAAAGATGAGTTCTGAACGTCGAAAAGAAAAGTCTAGAG
ATGCAGCAAGATCTCGGCGAAGCAAAGAGTCTGAAGTTTTTTATGAGCTTGCTCATCAGTTGCCACTTCC
CCACAATGTGAGCTCACATCTTGATAAAGCTTCTGTTATGAGGCTCACCATCAGTTATTTACGTGTGAGA
AAACTTCTGGATGCCGGTGGTCTAGACAGTGAAGATGAGATGAAGGCACAGATGGACTGTTTTTATCTGA
AAGCCCTAGATGGCTTTGTGATGGTGCTAACAGATGACGGCGACATGGTTTACATTTCTGATAACGTGAA
CAAATACATGGGGTTAACTCAGTTTGAACTAACTGGACACAGTGTGTTTGATTTTACTCATCCATGTGAC
CATGAGGAAATGAGAGAAATGCTTACACACAGAAATGGCCCAGTGAGAAAAGGGAAAGAACTAAACACAC
AGCGGAGCTTTTTTCTCAGAATGAAGTGCACCCTAACAAGCCGGGGGAGGACGATGAACATCAAGTCAGC
AACGTGGAAGGTGCTTCACTGCACGGGCCATATTCATGTCTATGATACCAACAGTAACCAACCTCAGTGT
GGGTACAAGAAACCACCCATGACGTGCTTGGTGCTGATTTGTGAACCCATTCCTCATCCGTCAAATATTG
AAATTCCTTTAGATAGCAAGACATTTCTCAGTCGACACAGCCTCGATATGAAATTTTCTTACTGTGATGA
AAGAATTACTGAGTTGATGGGTTATGAGCCGGAAGAACTTTTGGGCCGCTCAATTTATGAATATTATCAT
GCTTTGGATTCTGATCATCTGACCAAAACTCACCATGATATGTTTACTAAAGGACAAGTCACCACAGGAC
AGTACAGGATGCTTGCCAAAAGAGGTGGATATGTCTGGGTTGAAACTCAAGCAACTGTCATATATAATAC
GAAGAACTCCCAGCCACAGTGCATTGTGTGTGTGAATTATGTTGTAAGTGGTATTATTCAGCACGACTTG
ATTTTCTCCCTTCAACAAACAGAATCTGTGCTCAAACCAGTTGAATCTTCAGATAAGAAGATGACTCAGC
TGTTCACCAAAGTTGAATCAGAGGCTACAAGCTGCCTTTTTGATAAGCTTAAGAAGGAGCCTGATGCTCT
CACTCTGCTGGCTCCAGCTGCCGGCGACACCATCATCTCTCTGGATTTTGGCAGCGATGACACAGAAACT
GAAGATCAACAACTTGAAGATGTTCCATTATATAATGATGTAATGTTTCCCTCTTCTAATGAAAAATTAA
ATATAAACCTGGCAATGTCTCCTTTACCTTCATCGGAAACTCCAAAGCCACTTCGAAGTAGTGCTGATCC
TGCACTGAATCAAGAGGTTGCATTAAAATTAGAATCAAGTCCAGAGTCACTGGGACTTTCTTTTACCATG
CCCCAGATTCAAGATCAGCCAGCAAGTCCTTCTGATGGAAGCACTAGACAAAGTTCACCTGAGAGACTTC
TTCAGGAAAACGTAAACACTCCTAACTTTTCCCAGCCTAACAGTCCCAGTGAATATTGCTTTGATGTGGA
TAGCGATATGGTCAATGTATTCAAGTTGGAACTGGTGGAAAAACTGTTTGCTGAAGACACAGAGGCAAAG
AATCCATTTTCAACTCAGGACACTGATTTAGACTTGGAGATGCTGGCTCCCTATATCCCAATGGATGATG
ATTTCCAGTTACGTTCCTTTGATCAGTTGTCACCATTAGAGAGCAATTCTCCAAGCCCTCCAAGTATGAG
CACAGTTACTGGGTTCCAGCAGACCCAGTTACAGAAACCTACCATCACTGCCACTGCCACCACAACTGCC
ACCACTGATGAATCAAAAACAGAGACGAAGGACAATAAAGAAGATATTAAAATACTGATTGCATCTCCAT
CTTCTACCCAAGTACCTCAAGAAACGACCACTGCTAAGGCATCAGCATACAGTGGCACTCACAGTCGGAC
AGCCTCACCAGACAGAGCAGGAAAGAGAGTCATAGAACAGACAGACAAAGCTCATCCAAGGAGCCTTAAC
CTGTCTGCCACTTTGAATCAAAGAAATACTGTTCCTGAGGAAGAATTAAACCCAAAGACAATAGCTTCGC
AGAATGCTCAGAGGAAGCGAAAAATGGAACATGATGGCTCCCTTTTTCAAGCAGCAGGAATTGGAACATT
ATTGCAGCAACCGGGTGACTGTGCACCTACTATGTCACTTTCCTGGAAACGAGTGAAAGGATTCATATCT
AGTGAACAGAATGGAACGGAGCAAAAGACTATTATTTTAATACCCTCCGATTTAGCATGCAGACTGCTGG
GGCAGTCAATGGATGAGAGTGGATTACCACAGCTGACCAGTTACGATTGTGAAGTTAATGCTCCCATACA
AGGCAGCAGAAACCTACTGCAGGGTGAAGAATTACTCAGAGCTTTGGATCAAGTTAACTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_010431
Insert Size 2511 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC026139, AAH26139
RefSeq Size 4775 bp
RefSeq ORF 2511 bp
Locus ID 15251
UniProt ID Q61221
Gene Summary This gene encodes the alpha subunit which, along with the beta subunit, forms a heterodimeric transcription factor that regulates the cellular and developmental response to reduced oxygen tension. The transcription factor has been shown to regulate genes involved in several biological processes, including erythropoiesis and angiogenesis which aid in increased delivery of oxygen to hypoxic regions. The transcription factor also plays a role in the induction of genes involved in cell proliferation and survival, energy metabolism, apoptosis, and glucose and iron metabolism. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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