Ago3 (NM_153402) Mouse Untagged Clone

CAT#: MC222183

Ago3 (untagged) - Mouse eukaryotic translation initiation factor 2C, 3 (Eif2c3), (10ug)



  "NM_153402" in other vectors (4)

CNY 10,930.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
Eif2c3 Antibody - N-terminal region
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AW048688; C130014L07Rik; Eif2c3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222183 representing NM_153402
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAATCGGCTCCGCAGGACCCATTGGGGCCCAGCCCCTATTCATCGTGCCCAGAAGACCTGGGTATG
GCACTATGGGCAAACCCATTAAACTGTTGGCTAACTGTTTTCAAGTTGAAATCCCAAAGATTGATGTCTA
CCTCTATGAAGTTGACATCAAACCAGACAAGTGTCCTCGAAGAGTGAACAGAGAAGTAGTTGATTCAATG
GTTCAGCATTTTAAAGTGACTATATTTGGAGACCGTAGACCAGTTTATGATGGAAAAAGAAGCCTTTATA
CAGCCAATCCACTTCCTGTGGCAACCACTGGGGTAGATTTAGATGTTACATTACCTGGGGAAGGTGGAAA
AGATCGACCTTTCAAGGTGTCAGTCAAATTTGTGTCTCGAGTCAGCTGGCACCTACTACATGAAGCACTG
GCTGGAGGGACTTTGCCTGAGCCACTGGAATTAGACAAGCCTGTCAGCACTAACCCCGTCCATGCCGTTG
ACGTGGTGCTCCGACATCTGCCCTCTATGAAATATACGCCTGTGGGGCGTTCCTTTTTCTCAGCTCCAGA
AGGATATGACCACCCTCTGGGAGGGGGCAGGGAAGTATGGTTTGGATTCCATCAGTCTGTTCGGCCTGCC
ATGTGGAAAATGATGCTAAATATTGATGTTTCTGCCACTGCCTTTTACAAAGCACAACCTGTAATTCAGT
TCATGTGTGAAGTTCTTGACATTCACAACATTGATGAACAACCCAGACCCCTGACTGATTCTCATCGGGT
AAAATTCACTAAGGAGATAAAAGGTCTGAAAGTTGAAGTGACTCATTGTGGAACAATGAGACGGAAATAC
CGTGTTTGTAATGTAACGAGAAGGCCTGCCAGCCACCAAACCTTTCCTTTACAGCTAGAAAATGGCCAAA
CTGTGGAGAGGACTGTGGCTCAGTATTTCAGAGAAAAGTACACGCTGCAGCTGAAGTACCCACACCTTCC
CTGCCTGCAAGTGGGGCAGGAGCAGAAGCATACATACCTGCCACTAGAAGTCTGTAATATTGTGGCTGGG
CAAAGGTGTATCAAGAAACTAACGGACAATCAGACTTCTACAATGATAAAGGCAACAGCAAGATCTGCAC
CAGATAGACAAGAGGAAATTAGCAGATTGGTAAGAAGTGCAAATTATGAAACAGATCCATTTGTTCAGGA
GTTTCAGTTTAAAGTGCGGGATGAAATGGCCCATGTGACAGGACGTGTACTTCCAGCACCTATGCTCCAG
TACGGAGGACGGAACCGGACAGTAGCCACACCCAGCCACGGAGTATGGGACATGCGAGGAAAACAGTTCC
ACACAGGAGTCGAAATCAAAATGTGGGCTATTGCTTGCTTTGCCACACAGAGGCAGTGCAGAGAAGAAAT
ACTAAAAGGTTTCACAGACCAGCTTCGGAAGATTTCCAAGGATGCAGGGATGCCCATCCAGGGTCAGCCA
TGCTTCTGCAAATACGCACAGGGGGCAGACAGCGTGGAGCCCATGTTCCGGCATCTCAAGAACACCTACT
CAGGACTGCAGCTCATTATCGTCATCCTGCCCGGGAAGACACCAGTGTATGCGGAAGTGAAACGTGTGGG
AGATACACTTTTGGGTATGGCCACACAGTGTGTACAAGTCAAGAATGTAATAAAAACATCTCCTCAAACT
CTTTCAAACTTGTGCCTAAAGATCAATGTTAAACTTGGAGGAATCAATAATATTCTTGTACCTCATCAAA
GACCTTCTGTGTTCCAGCAACCAGTGATCTTCCTGGGCGCAGATGTCACACACCCGCCTGCTGGTGACGG
AAAAAAGCCCTCCATTGCTGCTGTTGTAGGTAGTATGGATGCCCATCCAAGCAGATACTGTGCCACAGTA
AGAGTTCAGAGACCCCGACAGGAAATCATCCAGGACTTGGCCTCCATGGTCCGGGAACTGCTCATCCAAT
TTTATAAATCAACCCGATTCAAACCTACTCGTATAATCTTCTATCGGGATGGTGTTTCAGAAGGACAGTT
CAGACAGGTATTATATTATGAGCTACTGGCAATTCGAGAAGCCTGTATCAGTTTGGAAAAAGACTATCAA
CCTGGAATAACCTACATTGTTGTTCAGAAGAGACATCATACACGGTTGTTCTGTGCTGATAGGACAGAAA
GGGTTGGAAGAAGCGGCAACATCCCAGCTGGAACCACGGTGGATACCGACATTACTCACCCATATGAATT
CGACTTTTATCTCTGTAGCCATGCTGGAATACAGGGTACCAGCCGTCCTTCACACTATCATGTTTTATGG
GATGACAACTTCTTTACTGCAGATGAACTTCAGCTGCTCACTTACCAGCTCTGTCACACTTACGTACGCT
GTACAAGATCTGTTTCTATCCCTGCACCAGCATATTACGCTCACCTGGTGGCATTCAGAGCCAGATATCA
TCTTGTGGACAAGGAACATGACAGTGCTGAAGGAAGCCATGTTTCAGGACAGAGCAATGGGCGAGATCCA
CAAGCTCTTGCCAAGGCTGTACAGATTCACCAAGATACCTTACGCACAATGTACTTCGCTTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_153402
Insert Size 2583 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_153402.2, NP_700451.2
RefSeq Size 7635 bp
RefSeq ORF 2583 bp
Locus ID 214150
UniProt ID Q8CJF9
Gene Summary Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (riRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent riRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4 (PubMed:19174539). Possesses RNA slicer activity but only on select RNAs bearing 5'- and 3'-flanking sequences to the region of guide-target complementarity (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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