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Ago3 (NM_153402) Mouse Untagged Clone
CAT＃: MC222183
Ago3 (untagged) - Mouse eukaryotic translation initiation factor 2C, 3 (Eif2c3), (10ug)
CNY 10,930.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | AW048688; C130014L07Rik; Eif2c3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222183 representing NM_153402
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAATCGGCTCCGCAGGACCCATTGGGGCCCAGCCCCTATTCATCGTGCCCAGAAGACCTGGGTATG GCACTATGGGCAAACCCATTAAACTGTTGGCTAACTGTTTTCAAGTTGAAATCCCAAAGATTGATGTCTA CCTCTATGAAGTTGACATCAAACCAGACAAGTGTCCTCGAAGAGTGAACAGAGAAGTAGTTGATTCAATG GTTCAGCATTTTAAAGTGACTATATTTGGAGACCGTAGACCAGTTTATGATGGAAAAAGAAGCCTTTATA CAGCCAATCCACTTCCTGTGGCAACCACTGGGGTAGATTTAGATGTTACATTACCTGGGGAAGGTGGAAA AGATCGACCTTTCAAGGTGTCAGTCAAATTTGTGTCTCGAGTCAGCTGGCACCTACTACATGAAGCACTG GCTGGAGGGACTTTGCCTGAGCCACTGGAATTAGACAAGCCTGTCAGCACTAACCCCGTCCATGCCGTTG ACGTGGTGCTCCGACATCTGCCCTCTATGAAATATACGCCTGTGGGGCGTTCCTTTTTCTCAGCTCCAGA AGGATATGACCACCCTCTGGGAGGGGGCAGGGAAGTATGGTTTGGATTCCATCAGTCTGTTCGGCCTGCC ATGTGGAAAATGATGCTAAATATTGATGTTTCTGCCACTGCCTTTTACAAAGCACAACCTGTAATTCAGT TCATGTGTGAAGTTCTTGACATTCACAACATTGATGAACAACCCAGACCCCTGACTGATTCTCATCGGGT AAAATTCACTAAGGAGATAAAAGGTCTGAAAGTTGAAGTGACTCATTGTGGAACAATGAGACGGAAATAC CGTGTTTGTAATGTAACGAGAAGGCCTGCCAGCCACCAAACCTTTCCTTTACAGCTAGAAAATGGCCAAA CTGTGGAGAGGACTGTGGCTCAGTATTTCAGAGAAAAGTACACGCTGCAGCTGAAGTACCCACACCTTCC CTGCCTGCAAGTGGGGCAGGAGCAGAAGCATACATACCTGCCACTAGAAGTCTGTAATATTGTGGCTGGG CAAAGGTGTATCAAGAAACTAACGGACAATCAGACTTCTACAATGATAAAGGCAACAGCAAGATCTGCAC CAGATAGACAAGAGGAAATTAGCAGATTGGTAAGAAGTGCAAATTATGAAACAGATCCATTTGTTCAGGA GTTTCAGTTTAAAGTGCGGGATGAAATGGCCCATGTGACAGGACGTGTACTTCCAGCACCTATGCTCCAG TACGGAGGACGGAACCGGACAGTAGCCACACCCAGCCACGGAGTATGGGACATGCGAGGAAAACAGTTCC ACACAGGAGTCGAAATCAAAATGTGGGCTATTGCTTGCTTTGCCACACAGAGGCAGTGCAGAGAAGAAAT ACTAAAAGGTTTCACAGACCAGCTTCGGAAGATTTCCAAGGATGCAGGGATGCCCATCCAGGGTCAGCCA TGCTTCTGCAAATACGCACAGGGGGCAGACAGCGTGGAGCCCATGTTCCGGCATCTCAAGAACACCTACT CAGGACTGCAGCTCATTATCGTCATCCTGCCCGGGAAGACACCAGTGTATGCGGAAGTGAAACGTGTGGG AGATACACTTTTGGGTATGGCCACACAGTGTGTACAAGTCAAGAATGTAATAAAAACATCTCCTCAAACT CTTTCAAACTTGTGCCTAAAGATCAATGTTAAACTTGGAGGAATCAATAATATTCTTGTACCTCATCAAA GACCTTCTGTGTTCCAGCAACCAGTGATCTTCCTGGGCGCAGATGTCACACACCCGCCTGCTGGTGACGG AAAAAAGCCCTCCATTGCTGCTGTTGTAGGTAGTATGGATGCCCATCCAAGCAGATACTGTGCCACAGTA AGAGTTCAGAGACCCCGACAGGAAATCATCCAGGACTTGGCCTCCATGGTCCGGGAACTGCTCATCCAAT TTTATAAATCAACCCGATTCAAACCTACTCGTATAATCTTCTATCGGGATGGTGTTTCAGAAGGACAGTT CAGACAGGTATTATATTATGAGCTACTGGCAATTCGAGAAGCCTGTATCAGTTTGGAAAAAGACTATCAA CCTGGAATAACCTACATTGTTGTTCAGAAGAGACATCATACACGGTTGTTCTGTGCTGATAGGACAGAAA GGGTTGGAAGAAGCGGCAACATCCCAGCTGGAACCACGGTGGATACCGACATTACTCACCCATATGAATT CGACTTTTATCTCTGTAGCCATGCTGGAATACAGGGTACCAGCCGTCCTTCACACTATCATGTTTTATGG GATGACAACTTCTTTACTGCAGATGAACTTCAGCTGCTCACTTACCAGCTCTGTCACACTTACGTACGCT GTACAAGATCTGTTTCTATCCCTGCACCAGCATATTACGCTCACCTGGTGGCATTCAGAGCCAGATATCA TCTTGTGGACAAGGAACATGACAGTGCTGAAGGAAGCCATGTTTCAGGACAGAGCAATGGGCGAGATCCA CAAGCTCTTGCCAAGGCTGTACAGATTCACCAAGATACCTTACGCACAATGTACTTCGCTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_153402 |
| Insert Size | 2583 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_153402.2, NP_700451.2 |
| RefSeq Size | 7635 bp |
| RefSeq ORF | 2583 bp |
| Locus ID | 214150 |
| UniProt ID | Q8CJF9 |
| Gene Summary | Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (riRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent riRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4 (PubMed:19174539). Possesses RNA slicer activity but only on select RNAs bearing 5'- and 3'-flanking sequences to the region of guide-target complementarity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG221842 | Ago3 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 2C 3 (Eif2c3), (10ug) |
CNY 7,890.00 |
|
| MR221842 | Ago3 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2C, 3 (Eif2c3) |
CNY 6,304.00 |
|
| MR221842L3 | Lenti ORF clone of Ago3 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2C, 3 (Eif2c3) |
CNY 9,030.00 |
|
| MR221842L4 | Lenti ORF clone of Ago3 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 2C, 3 (Eif2c3) |
CNY 9,030.00 |
