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Tlr5 (NM_016928) Mouse Untagged Clone
CAT＃: MC222323
Tlr5 (untagged) - Mouse toll-like receptor 5 (Tlr5), (10ug)
CNY 9,600.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222323 representing NM_016928
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGCTGAGTTCCCCCACGCACCACACTTCAGCAGGATCATGGCATGTCAACTTGACTTGCTCATAG GTGTGATCTTCATGGCCAGCCCCGTGTTGGTAATATCTCCCTGTTCTTCAGACGGCAGGATAGCCTTTTT CCGAGGCTGTAACCTCACCCAGATTCCCTGGATCCTCAATACTACCACTGAGAGGCTCCTGCTCAGCTTC AACTATATCAGTATGGTGGTTGCCACATCATTTCCACTCCTGGAGCGGCTCCAGTTGCTGGAGCTGGGGA CCCAGTATGCTAACTTGACCATTGGTCCAGGGGCTTTCAGAAACCTGCCCAATCTTAGGATCTTGGACTT GGGCCAAAGCCAGATCGAAGTCTTGAATCGAGATGCCTTTCAAGGTCTGCCCCATCTCTTGGAACTTCGG CTGTTTTCCTGTGGACTCTCCAGTGCTGTGTTAAGTGACGGTTACTTCAGAAATCTATATTCATTAGCTC GCTTAGACCTATCTGGCAACCAGATTCACAGCCTCCGCCTCCATTCTTCATTCCGGGAACTGAATTCCTT AAGCGACGTAAATTTTGCTTTCAACCAAATATTCACTATATGTGAAGATGAACTCGAGCCTCTGCAGGGC AAAACACTGTCTTTCTTTGGCCTCAAATTAACTAAGCTGTTCAGCAGAGTCTCTGTGGGCTGGGAGACAT GCAGGAACCCCTTCAGAGGCGTGAGGCTAGAAACTCTAGATCTTTCTGAAAATGGCTGGACGGTGGACAT CACAAGGAACTTCAGCAACATCATCCAGGGAAGCCAGATTTCCTCTTTGATTCTTAAACACCACATCATG GGTCCTGGCTTTGGCTTCCAGAACATCAGAGATCCTGACCAGAGCACATTTGCCAGCCTGGCCAGAAGTT CGGTGCTGCAACTGGACCTTTCGCACGGCTTTATCTTCTCCTTGAATCCTCGACTGTTTGGGACACTGAA GGATTTGAAGATGCTGAACCTTGCCTTCAACAAGATAAACAAGATTGGAGAGAATGCCTTTTATGGGCTT GACAGCCTCCAGGTTCTCAATCTATCCTATAATCTTTTGGGGGAACTCTATAATTCCAACTTCTATGGGC TTCCTAGAGTAGCCTACGTTGACCTTCAAAGGAACCACATTGGGATCATTCAAGACCAAACATTCAGATT ATTAAAAACGTTACAAACCTTAGATCTCCGTGACAATGCTCTTAAGGCCATTGGTTTTATTCCAAGCATA CAGATGGTCCTCCTGGGAGGCAATAAGCTGGTCCATTTGCCACACATCCACTTTACTGCCAACTTCCTAG AGTTATCTGAAAACAGGCTAGAAAACCTGTCCGACCTCTACTTCCTCCTGCGAGTCCCCCAGCTCCAGTT TCTCATCTTGAATCAGAATCGCCTTTCGTCATGCAAGGCAGCCCACACTCCCTCGGAGAACCCAAGCTTA GAACAGCTTTTCCTTACAGAGAATATGCTGCAGCTGGCCTGGGAGACCGGCCTCTGTTGGGATGTTTTTC AAGGCCTTTCCCGCCTCCAGATTCTTTACCTGAGTAATAACTACCTTAATTTCCTTCCACCTGGGATATT TAACGACCTGGTTGCATTACGGATGCTTAGTCTTAGTGCTAACAAGCTGACCGTGCTCTCTCCGGGCAGT TTACCTGCTAATTTAGAGATTCTCGACATATCTAGAAATCAGCTTTTCTCTCCTGACCCTGCTTTGTTTT CTTCGCTTCGTGTTTTGGACATAACTCATAACGAGTTCGTCTGCAACTGTGAACTTAGCACTTTTATCTC CTGGCTCAACCAAACCAACGTCACCCTGTTCGGCTCTCCTGCAGACGTGTATTGCATGTACCCTAACTCA CTGCTAGGGGGCTCCCTCTACAACATATCCACCGAAGACTGCGATGAAGAGGAAGCCATGCGGTCCCTAA AGTTTTCCCTTTTCATCCTGTGCACGGTCACTTTGACTCTATTCCTCGTCATCACCCTTGTAGTCATAAA GTTCCGGGGAATCTGTTTCCTGTGCTATAAGACCATCCAGAAGCTGGTGTTCAAGGACAAGGTCTGGAGT TTGGAACCTGGTGCATATAGATATGATGCCTACTTCTGCTTCAGCAGCAAAGACTTTGAATGGGCACAGA ATGCTTTGCTCAAACACCTGGATGCTCACTACAGTTCCCGAAACAGGCTCAGGCTATGCTTTGAAGAAAG AGACTTCATTCCAGGGGAAAACCATATCTCCAACATCCAGGCGGCTGTCTGGGGCAGCAGGAAGACGGTG TGTCTAGTGAGCAGACACTTCCTGAAGGATGGTTGGTGCCTGGAGGCCTTCAGGTATGCCCAGAGCCGGA GTCTGTCTGATCTCAAGAGCATTCTCATCGTGGTGGTGGTGGGATCGCTGTCCCAGTATCAGCTGATGAG ACATGAGACCATCAGAGGGTTTCTGCAAAAGCAACAGTACTTGAGGTGGCCTGAAGACCTCCAGGATGTT GGCTGGTTTCTCGATAAACTCTCCGGATGCATTCTAAAGGAAGAAAAAGGAAAGAAAAGAAGCAGTTCCA TCCAGTTGCGAACCATAGCAACCATTTCCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_016928 |
| Insert Size | 2622 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_016928.2, NP_058624.2 |
| RefSeq Size | 3211 bp |
| RefSeq ORF | 2622 bp |
| Locus ID | 53791 |
| UniProt ID | Q9JLF7 |
| Gene Summary | Pattern recognition receptor (PRR) located on the cell surface that participates in the activation of innate immunity and inflammatory response. Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota. Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF-kappa-B activation, cytokine secretion and induction of the inflammatory response. Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG225590 | Tlr5 (tGFP-tagged) - Mouse toll-like receptor 5 (Tlr5), (10ug) |
CNY 11,192.00 |
|
| MR225590 | Tlr5 (Myc-DDK-tagged) - Mouse toll-like receptor 5 (Tlr5) |
CNY 9,592.00 |
|
| MR225590L3 | Lenti ORF clone of Tlr5 (Myc-DDK-tagged) - Mouse toll-like receptor 5 (Tlr5) |
CNY 10,640.00 |
|
| MR225590L4 | Lenti ORF clone of Tlr5 (mGFP-tagged) - Mouse toll-like receptor 5 (Tlr5) |
CNY 10,640.00 |
