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Tlr5 (NM_016928) Mouse Tagged ORF Clone
CAT＃: MR225590
- TrueORF®
Tlr5 (Myc-DDK-tagged) - Mouse toll-like receptor 5 (Tlr5)
ORF Plasmid: tGFP
"NM_016928" in other vectors (4)
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CNY 9,592.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225590 representing NM_016928
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGCTGAGTTCCCCCACGCACCACACTTCAGCAGGATCATGGCATGTCAACTTGACTTGCTCATAG GTGTGATCTTCATGGCCAGCCCCGTGTTGGTAATATCTCCCTGTTCTTCAGACGGCAGGATAGCCTTTTT CCGAGGCTGTAACCTCACCCAGATTCCCTGGATCCTCAATACTACCACTGAGAGGCTCCTGCTCAGCTTC AACTATATCAGTATGGTGGTTGCCACATCATTTCCACTCCTGGAGCGGCTCCAGTTGCTGGAGCTGGGGA CCCAGTATGCTAACTTGACCATTGGTCCAGGGGCTTTCAGAAACCTGCCCAATCTTAGGATCTTGGACTT GGGCCAAAGCCAGATCGAAGTCTTGAATCGAGATGCCTTTCAAGGTCTGCCCCATCTCTTGGAACTTCGG CTGTTTTCCTGTGGACTCTCCAGTGCTGTGTTAAGTGACGGTTACTTCAGAAATCTATATTCATTAGCTC GCTTAGACCTATCTGGCAACCAGATTCACAGCCTCCGCCTCCATTCTTCATTCCGGGAACTGAATTCCTT AAGCGACGTAAATTTTGCTTTCAACCAAATATTCACTATATGTGAAGATGAACTCGAGCCTCTGCAGGGC AAAACACTGTCTTTCTTTGGCCTCAAATTAACTAAGCTGTTCAGCAGAGTCTCTGTGGGCTGGGAGACAT GCAGGAACCCCTTCAGAGGCGTGAGGCTAGAAACTCTAGATCTTTCTGAAAATGGCTGGACGGTGGACAT CACAAGGAACTTCAGCAACATCATCCAGGGAAGCCAGATTTCCTCTTTGATTCTTAAACACCACATCATG GGTCCTGGCTTTGGCTTCCAGAACATCAGAGATCCTGACCAGAGCACATTTGCCAGCCTGGCCAGAAGTT CGGTGCTGCAACTGGACCTTTCGCACGGCTTTATCTTCTCCTTGAATCCTCGACTGTTTGGGACACTGAA GGATTTGAAGATGCTGAACCTTGCCTTCAACAAGATAAACAAGATTGGAGAGAATGCCTTTTATGGGCTT GACAGCCTCCAGGTTCTCAATCTATCCTATAATCTTTTGGGGGAACTCTATAATTCCAACTTCTATGGGC TTCCTAGAGTAGCCTACGTTGACCTTCAAAGGAACCACATTGGGATCATTCAAGACCAAACATTCAGATT ATTAAAAACGTTACAAACCTTAGATCTCCGTGACAATGCTCTTAAGGCCATTGGTTTTATTCCAAGCATA CAGATGGTCCTCCTGGGAGGCAATAAGCTGGTCCATTTGCCACACATCCACTTTACTGCCAACTTCCTAG AGTTATCTGAAAACAGGCTAGAAAACCTGTCCGACCTCTACTTCCTCCTGCGAGTCCCCCAGCTCCAGTT TCTCATCTTGAATCAGAATCGCCTTTCGTCATGCAAGGCAGCCCACACTCCCTCGGAGAACCCAAGCTTA GAACAGCTTTTCCTTACAGAGAATATGCTGCAGCTGGCCTGGGAGACCGGCCTCTGTTGGGATGTTTTTC AAGGCCTTTCCCGCCTCCAGATTCTTTACCTGAGTAATAACTACCTTAATTTCCTTCCACCTGGGATATT TAACGACCTGGTTGCATTACGGATGCTTAGTCTTAGTGCTAACAAGCTGACCGTGCTCTCTCCGGGCAGT TTACCTGCTAATTTAGAGATTCTCGACATATCTAGAAATCAGCTTTTCTCTCCTGACCCTGCTTTGTTTT CTTCGCTTCGTGTTTTGGACATAACTCATAACGAGTTCGTCTGCAACTGTGAACTTAGCACTTTTATCTC CTGGCTCAACCAAACCAACGTCACCCTGTTCGGCTCTCCTGCAGACGTGTATTGCATGTACCCTAACTCA CTGCTAGGGGGCTCCCTCTACAACATATCCACCGAAGACTGCGATGAAGAGGAAGCCATGCGGTCCCTAA AGTTTTCCCTTTTCATCCTGTGCACGGTCACTTTGACTCTATTCCTCGTCATCACCCTTGTAGTCATAAA GTTCCGGGGAATCTGTTTCCTGTGCTATAAGACCATCCAGAAGCTGGTGTTCAAGGACAAGGTCTGGAGT TTGGAACCTGGTGCATATAGATATGATGCCTACTTCTGCTTCAGCAGCAAAGACTTTGAATGGGCACAGA ATGCTTTGCTCAAACACCTGGATGCTCACTACAGTTCCCGAAACAGGCTCAGGCTATGCTTTGAAGAAAG AGACTTCATTCCAGGGGAAAACCATATCTCCAACATCCAGGCGGCTGTCTGGGGCAGCAGGAAGACGGTG TGTCTAGTGAGCAGACACTTCCTGAAGGATGGTTGGTGCCTGGAGGCCTTCAGGTATGCCCAGAGCCGGA GTCTGTCTGATCTCAAGAGCATTCTCATCGTGGTGGTGGTGGGATCGCTGTCCCAGTATCAGCTGATGAG ACATGAGACCATCAGAGGGTTTCTGCAAAAGCAACAGTACTTGAGGTGGCCTGAAGACCTCCAGGATGTT GGCTGGTTTCTCGATAAACTCTCCGGATGCATTCTAAAGGAAGAAAAAGGAAAGAAAAGAAGCAGTTCCA TCCAGTTGCGAACCATAGCAACCATTTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225590 representing NM_016928
Red=Cloning site Green=Tags(s) MDAEFPHAPHFSRIMACQLDLLIGVIFMASPVLVISPCSSDGRIAFFRGCNLTQIPWILNTTTERLLLSF NYISMVVATSFPLLERLQLLELGTQYANLTIGPGAFRNLPNLRILDLGQSQIEVLNRDAFQGLPHLLELR LFSCGLSSAVLSDGYFRNLYSLARLDLSGNQIHSLRLHSSFRELNSLSDVNFAFNQIFTICEDELEPLQG KTLSFFGLKLTKLFSRVSVGWETCRNPFRGVRLETLDLSENGWTVDITRNFSNIIQGSQISSLILKHHIM GPGFGFQNIRDPDQSTFASLARSSVLQLDLSHGFIFSLNPRLFGTLKDLKMLNLAFNKINKIGENAFYGL DSLQVLNLSYNLLGELYNSNFYGLPRVAYVDLQRNHIGIIQDQTFRLLKTLQTLDLRDNALKAIGFIPSI QMVLLGGNKLVHLPHIHFTANFLELSENRLENLSDLYFLLRVPQLQFLILNQNRLSSCKAAHTPSENPSL EQLFLTENMLQLAWETGLCWDVFQGLSRLQILYLSNNYLNFLPPGIFNDLVALRMLSLSANKLTVLSPGS LPANLEILDISRNQLFSPDPALFSSLRVLDITHNEFVCNCELSTFISWLNQTNVTLFGSPADVYCMYPNS LLGGSLYNISTEDCDEEEAMRSLKFSLFILCTVTLTLFLVITLVVIKFRGICFLCYKTIQKLVFKDKVWS LEPGAYRYDAYFCFSSKDFEWAQNALLKHLDAHYSSRNRLRLCFEERDFIPGENHISNIQAAVWGSRKTV CLVSRHFLKDGWCLEAFRYAQSRSLSDLKSILIVVVVGSLSQYQLMRHETIRGFLQKQQYLRWPEDLQDV GWFLDKLSGCILKEEKGKKRSSSIQLRTIATIS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016928 |
| ORF Size | 2619 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016928.2, NP_058624.2 |
| RefSeq Size | 3211 bp |
| RefSeq ORF | 2622 bp |
| Locus ID | 53791 |
| UniProt ID | Q9JLF7 |
| MW | 99.7 kDa |
| Gene Summary | Pattern recognition receptor (PRR) located on the cell surface that participates in the activation of innate immunity and inflammatory response. Recognizes small molecular motifs named pathogen-associated molecular pattern (PAMPs) expressed by pathogens and microbe-associated molecular patterns (MAMPs) usually expressed by resident microbiota. Upon ligand binding such as bacterial flagellins, recruits intracellular adapter proteins MYD88 and TRIF leading to NF-kappa-B activation, cytokine secretion and induction of the inflammatory response. Plays thereby an important role in the relationship between the intestinal epithelium and enteric microbes and contributes to the gut microbiota composition throughout life.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222323 | Tlr5 (untagged) - Mouse toll-like receptor 5 (Tlr5), (10ug) |
CNY 9,600.00 |
|
| MG225590 | Tlr5 (tGFP-tagged) - Mouse toll-like receptor 5 (Tlr5), (10ug) |
CNY 11,192.00 |
|
| MR225590L3 | Lenti ORF clone of Tlr5 (Myc-DDK-tagged) - Mouse toll-like receptor 5 (Tlr5) |
CNY 10,640.00 |
|
| MR225590L4 | Lenti ORF clone of Tlr5 (mGFP-tagged) - Mouse toll-like receptor 5 (Tlr5) |
CNY 10,640.00 |
