Hcn1 (NM_010408) Mouse Untagged Clone

CAT#: MC222514

Hcn1 (untagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 1 (Hcn1), (10ug)



  "NM_010408" in other vectors (6)

CNY 10,008.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Bcng1; C630013B14Rik; HAC2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222514 representing NM_010408
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAAGGCGGCGGCAAACCCAACTCCGCGTCCAACAGCCGCGACGATGGCAACAGCGTCTTCCCCTCCA
AGGCGCCCGCGACGGGGCCGGTGGCGGCCGACAAGCGCCTGGGGACCCCGCCGGGGGGCGGCGCGGCCGG
GAAGGAACATGGCAACTCCGTGTGCTTCAAGGTGGACGGCGGCGGAGGAGAGGAGCCGGCGGGCAGCTTC
GAGGATGCCGAGGGGCCCCGGCGGCAGTATGGTTTCATGCAGAGGCAGTTCACCTCCATGCTGCAGCCTG
GGGTCAACAAATTCTCCCTCCGCATGTTTGGGAGCCAGAAGGCGGTGGAGAAGGAGCAGGAAAGGGTTAA
AACTGCAGGCTTCTGGATTATCCATCCGTACAGTGACTTCAGGTTTTATTGGGATTTAATCATGCTTATA
ATGATGGTTGGAAATTTGGTCATCATACCAGTTGGAATCACGTTCTTCACAGAGCAGACGACAACACCGT
GGATTATTTTCAACGTGGCATCCGATACTGTTTTCCTGTTGGACTTAATCATGAATTTTAGGACTGGGAC
TGTCAATGAAGACAGCTCGGAAATCATCCTGGACCCTAAAGTGATCAAGATGAATTATTTAAAAAGCTGG
TTTGTGGTGGACTTCATCTCATCGATCCCGGTGGATTATATCTTTCTCATTGTAGAGAAAGGGATGGACT
CAGAAGTTTACAAGACAGCCAGAGCACTTCGTATCGTGAGGTTTACAAAAATTCTCAGTCTCTTGCGGTT
ATTACGCCTTTCAAGGTTAATCAGATACATACACCAGTGGGAAGAGATATTCCACATGACCTATGACCTC
GCCAGTGCTGTGGTGAGGATCTTCAACCTCATTGGCATGATGCTGCTTCTGTGCCACTGGGATGGCTGTC
TTCAGTTCCTGGTTCCCCTGCTGCAGGACTTCCCACCAGATTGCTGGGTTTCTCTGAATGAAATGGTTAA
TGATTCCTGGGGAAAACAATATTCCTACGCACTCTTCAAAGCTATGAGTCACATGCTGTGCATTGGTTAT
GGCGCCCAAGCCCCTGTCAGCATGTCTGACCTCTGGATTACCATGCTGAGCATGATTGTGGGCGCCACCT
GCTACGCAATGTTTGTTGGCCATGCCACAGCTTTGATCCAGTCTTTGGACTCTTCAAGGAGGCAGTATCA
AGAGAAGTATAAGCAAGTAGAGCAATACATGTCATTCCACAAGTTACCAGCTGACATGCGCCAGAAGATA
CATGATTACTATGAGCACCGATACCAAGGCAAGATCTTCGATGAAGAAAATATTCTCAGTGAGCTTAATG
ATCCTCTGAGAGAGGAAATAGTCAACTTCAACTGCCGGAAACTGGTGGCAACTATGCCTCTTTTTGCTAA
CGCCGATCCCAATTTCGTGACGGCCATGCTGAGCAAGCTGAGATTTGAGGTGTTCCAGCCCGGAGACTAT
ATCATTCGAGAAGGAGCTGTGGGGAAGAAAATGTATTTCATCCAGCACGGTGTTGCTGGCGTTATCACCA
AGTCCAGTAAAGAAATGAAGCTGACAGATGGCTCTTACTTCGGAGAGATATGCCTGCTGACCAAGGGCCG
GCGCACTGCCAGTGTCCGAGCTGATACCTACTGTCGTCTTTACTCCCTTTCGGTGGACAATTTCAATGAG
GTCTTGGAGGAATATCCAATGATGAGAAGAGCCTTTGAGACAGTTGCTATTGACCGACTCGATCGGATAG
GCAAGAAAAACTCTATTCTCCTGCAGAAGTTCCAGAAGGATCTAAACACTGGTGTTTTCAACAACCAGGA
GAACGAGATCCTGAAGCAGATCGTGAAGCATGACCGAGAGATGGTACAAGCTATCCCTCCAATCAACTAT
CCTCAAATGACAGCCCTCAACTGCACATCTTCAACCACCACCCCAACCTCCCGCATGAGGACCCAATCTC
CGCCAGTCTACACCGCAACCAGCCTGTCTCACAGCAATCTGCACTCACCCAGTCCCAGCACACAGACGCC
CCAACCCTCAGCCATCCTTTCACCCTGCTCCTATACCACAGCAGTCTGCAGTCCTCCTATACAGAGCCCC
CTGGCCACACGAACTTTCCATTATGCCTCTCCCACTGCGTCCCAGCTGTCACTCATGCAGCAGCCTCAGC
AGCAACTACCGCAGTCCCAGGTACAGCAGACTCAGACTCAGACTCAGCAGCAGCAGCAGCAACAGCAGCA
GCAGCAGCAGCAGCAACAGCAACAACAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAG
CAGCAGCAGCAGCAGCCACAGACACCTGGTAGCTCCACACCGAAAAATGAAGTGCACAAGAGCACACAAG
CCCTTCATAACACCAACCTGACCAAAGAAGTCAGGCCCCTTTCCGCCTCGCAGCCTTCTCTGCCCCATGA
GGTCTCCACTTTGATCTCCAGACCTCATCCCACTGTGGGCGAATCCCTGGCCTCTATCCCTCAACCCGTG
GCAGCAGTCCACAGCACTGGCCTTCAGGCAGGGAGCAGGAGCACAGTGCCACAACGTGTCACCTTGTTCC
GACAGATGTCCTCGGGAGCCATCCCCCCCAACCGAGGAGTGCCTCCAGCACCCCCTCCACCAGCAGCTGT
GCAGAGAGAGTCTCCCTCAGTCCTAAATACAGACCCAGATGCAGAAAAACCCCGTTTTGCTTCGAATTTA
TGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_010408
Insert Size 2733 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_010408.3, NP_034538.2
RefSeq Size 7911 bp
RefSeq ORF 2733 bp
Locus ID 15165
UniProt ID O88704
Gene Summary Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). May mediate responses to sour stimuli.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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