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Pcsk5 (NM_001163144) Mouse Untagged Clone
CAT＃: MC222581
Pcsk5 (untagged) - Mouse proprotein convertase subtilisin/kexin type 5 (Pcsk5), transcript variant 2, (10ug)
CNY 11,780.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | b2b585C; b2b585Clo; b2b1549C; b2b1549Clo; PC; PC5; PC5/; PC6; SP; SPC6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222581 representing NM_001163144
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTGGGACTGGGGGAACCGCTGCAGCCGCCCGGGACGGCGGGACCTGCTGTGCGTGCTGGCACTGC TCGCCGGCTGTCTGCTCCCGGTATGCCGGACGCGCGTCTACACCAACCACTGGGCAGTGAAGATCGCCGG CGGCTTCGCGGAGGCAGATCGCATAGCCAGCAAGTACGGATTCATCAACGTAGGACAGATCGGTGCACTG AAGGACTACTATCACTTCTACCATAGTAGGACCATTAAAAGGTCTGTTCTCTCGAGCAGAGGAACCCACA GTTTCATTTCAATGGAACCAAAGGTGGAGTGGATCCAACAGCAAGTGGTGAAAAAAAGAACCAAGAGGGA TTATGACCTCAGCCATGCCCAGTCAACCTACTTCAATGATCCCAAGTGGCCAAGTATGTGGTACATGCAC TGCAGTGACAACACCCATCCTTGCCAGTCAGACATGAATATCGAAGGAGCCTGGAAGAGAGGCTACACGG GGAAGAATATCGTGGTGACTATCCTGGATGACGGCATCGAGAGAACCCACCCAGATCTGATGCAAAACTA CGATGCTCTGGCAAGTTGCGATGTGAATGGGAATGACTTGGACCCGATGCCTCGTTATGATGCAAGCAAT GAGAACAAGCATGGGACCCGCTGTGCCGGAGAAGTGGCAGCCACTGCAAACAACTCTCACTGCACTGTCG GGATCGCTTTCAACGCCAAGATTGGAGGTGTGAGAATGCTGGATGGTGATGTCACTGACATGGTGGAGGC AAAGTCTGTCAGCTACAACCCACAGCATGTGCACATCTACAGTGCCAGCTGGGGACCAGATGACGATGGC AAGACTGTGGATGGGCCAGCTCCCCTCACCCGGCAAGCCTTTGAGAACGGCGTGAGAATGGGGCGGAGAG GCCTTGGATCTGTGTTTGTGTGGGCATCTGGCAATGGTGGACGGAGCAAGGATCACTGTTCTTGTGATGG CTATACCAACAGCATCTATACCATCTCCATCAGCAGTACGGCCGAAAGTGGAAAGAAACCTTGGTACTTG GAAGAGTGTTCATCTACACTGGCTACAACCTACAGCAGTGGAGAATCCTATGATAAGAAAATAATCACTA CTGATCTAAGGCAGCGATGCACAGACAATCACACTGGAACATCAGCCTCAGCCCCCATGGCTGCTGGAAT CATTGCCCTGGCCCTAGAAGCCAATCCGTTTCTGACCTGGAGAGACGTGCAGCATGTTATTGTCAGGACT TCCCGTGCGGGACATTTGAACGCTAATGACTGGAAAACCAATGCTGCTGGTTTTAAGGTGAGCCATCTCT ATGGATTTGGACTGATGGATGCCGAAGCCATGGTGATGGAAGCAGAGAAGTGGACAACTGTTCCTCAGCA GCACGTGTGTGTGGAAAGCACAGACCGACAAATCAAGACCATTCGACCAAACAGTGCAGTGCGCTCCATC TACAAAGCCTCAGGCTGCTCGGATAATCCCAACCATCACGTCAATTACCTGGAGCATGTAGTTGTGCGTA TTACCATCACACACCCACGGAGGGGAGACCTGGCCATCTATCTGACATCACCCTCAGGAACCAGATCCCA GCTCTTGGCCAACAGGCTCTTTGATCATTCCATGGAAGGGTTTAAGAACTGGGAGTTCATGACTATTCAT TGCTGGGGAGAACGGGCTGCTGGGGACTGGGTCCTCGAAGTTTATGATACGCCATCTCAGCTGAGGAACT TCAAGACTCCAGGTAAATTGAAAGAATGGTCCTTAGTCCTCTATGGCACGTCCGTACAGCCATACTCCCC AACCAACGAGTTTCCCAAAGTGGAACGCTTCCGCTACAGCCGAGTGGAAGACCCCACAGATGACTACGGT GCTGAAGATTATGCAGGTCCCTGTGACCCTGAATGCAGTGAGGTTGGATGTGACGGGCCAGGACCAGATC ACTGCAGTGACTGCTTACACTACTACTACAAGCTGAAAAATAACACCAGAATCTGTGTCTCCAGCTGCCC TCCTGGCCACTACCATGCTGACAAGAAGAGATGCCGGAAGTGTGCCCCAAACTGCGAGTCCTGCTTTGGC AGCCATGGTAATCAGTGCCTCTCCTGTAAATATGGCTACTTCCTGAATGAAGAAACTAGCAGCTGTGTTA CTCAGTGCCCTGATGGATCATACGAGGATATCAAGAAAAATGTCTGTGGGAAATGCAGTGAGAACTGCAA GGCATGCATTGGATTTCACAACTGCACAGAGTGCAAGGGCGGGTTAAGTCTTCAGGGATCCCGCTGTTCG GTCACCTGCGAGGATGGACAGTTCTTCAATGGTCACGACTGCCAGCCCTGCCATCGCTTCTGTGCTACTT GCTCTGGGGCCGGAGCAGATGGATGTATTAACTGCACCGAGGGGTATGTCATGGAAGAGGGAAGGTGTGT ACAAAGTTGTAGTGTGAGCTACTACTTGGACCACTCTTCAGAGGGTGGCTACAAATCCTGCAAGAGATGT GATAACAGCTGTTTGACATGCAATGGGCCAGGATTCAAGAACTGTTCCAGCTGCCCCAGTGGATATCTTT TAGACTTAGGAACGTGTCAGATGGGAGCCATCTGCAAGGATGCTACGGAAGAGTCCTGGGCAGAAGGAGG CTTCTGTATGCTTGTGAAAAAGAACAATCTCTGTCAGCGAAAAGTTCTACAACAGCTGTGCTGCAAAACA TGTACATTCCAAGGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_001163144 |
| Insert Size | 2748 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_001163144.1, NP_001156616.1 |
| RefSeq Size | 5783 bp |
| RefSeq ORF | 2748 bp |
| Locus ID | 18552 |
| Gene Summary | This gene encodes a subtilisin-like proprotein convertase that mediates posttranslational endoproteolytic processing of various proprotein substrates traversing the secretory pathway. The encoded protein is an inactive zymogen that undergoes autoproteolytic processing in the endoplasmic reticulum and the Golgi network to generate an active enzyme. Mice lacking the encoded protein die at an early embryonic stage. Conditional inactivation this gene in the epiblast but not in the extraembryonic tissue bypasses embryonic lethality but results in death at birth. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2015] Transcript Variant: This variant (2, also known as PC6A) represents use of an alternate 3' coding region and 3' UTR, compared to variant 1. The resulting isoform (2) has a substantially shorter and distinct C-terminus, compared to isoform 1. This isoform (2) may undergo proteolytic processing similar to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG222003 | Pcsk5 (tGFP-tagged) - Mouse proprotein convertase subtilisin/kexin type 5 (Pcsk5) transcript variant 2, (10ug) |
CNY 8,930.00 |
|
| MR222003 | Pcsk5 (Myc-DDK-tagged) - Mouse proprotein convertase subtilisin/kexin type 5 (Pcsk5), transcript variant 2 |
CNY 6,704.00 |
|
| MR222003L3 | Lenti ORF clone of Pcsk5 (Myc-DDK-tagged) - Mouse proprotein convertase subtilisin/kexin type 5 (Pcsk5), transcript variant 2 |
CNY 9,980.00 |
|
| MR222003L4 | Lenti ORF clone of Pcsk5 (mGFP-tagged) - Mouse proprotein convertase subtilisin/kexin type 5 (Pcsk5), transcript variant 2 |
CNY 9,980.00 |
