Rgs3 (NM_134257) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4930506N09Rik; C2pa; C2PA-RGS3; PDZ-RGS3; RGS3S
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC222872 representing NM_134257 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCGCTTCAATGGGCTCTGCAAAGTGTGTTCAGAACGCCGCTACCGGCAGATCACCATCCGGAGGG GCAAAGACGGCTTTGGCTTCACCATCTGCTGTGACTCTCCGGTCCGAGTCCAGGCTGTGGATTCTGGGGG CCCGGCAGAGAGGGCGGGACTGCAGCAGCTGGACACAGTGCTACAACTGAATGAGAGACCCGTGGAGCAC TGGAAATGTGTGGAGCTGGCACATGAGATCCGGAGCTGTCCTAGCGAGATCATCCTGCTCGTGTGGCGTG TGGTCCCCCAGATCAAGCCGGGGCCAGATGGCGGAGTCTTGCGGCGGGCCTCCTGCAAGTCCACACATGA CCTCCTGTCACCCCCTAACAAGAGGGAGAAGAACTGTACTCATGGGGCCCCAGTTCGTCCTGAGCAGCGC CACAGCTGCCACCTGGTGTGTGACAGCTCTGATGGTCTACTGCTTGGTGGCTGGGAGCGCTACACTGAGG TGGGCAAGCGCAGTGGCCAGCACACCCTGCCTGCACTGTCCCGGACCACCACCCCTACTGACCCCAACTA CATCATCCTGGCCCCACTGAATCCTGGAAGCCAGTTGCTGCGGCCTGTGTACCAGGAGGATACAATCCCT GAAGAACCGGGGACTACTACTAAAGGGAAATCGTACACCGGCCTGGGCAAGAAGTCTCGGCTCATGAAGA CAGTGCAGACCATGAAGGGCCACAGTAACTACCAAGACTGCTCAGCCCTGAGACCGCACATCCCGCATTC CAGTTACGGCACCTATGTCACCCTGGCCCCTAAAGTCCTGGTGTTCCCTGTCTTTGTGCAGCCCCTAGAT CTCTGTAACCCTGCCCGGACTCTCCTGCTGTCGGAGGAGCTGCTGCTGTATGAGGGGAGGAACAAGACTT CCCAGGTGACACTGTTTGCCTACTCGGACCTGCTGCTGTTCACTAAGGAGGAGGAGCCAGGCCGCTGCGA CGTCCTGAGAAATCCCCTCTACCTCCAGAGCGTGAAGCTACAGGAGGGCTCTTCAGAAGACTTGAAATTC TGTGTGCTGTACCTGGCAGAGAAGGCAGAGTGCTTATTCACTTTGGAGGCACACTCGCAGGAGCAGAAGA AGAGAGTGTGCTGGTGCCTGTCGGAGAACATCGCCAAGCAGCAACAGCTGGCCGCACCACCTACAGAGAG GAAGATGTTTGAGACAGAGGCAGATGAGAAGGAGATGCCCCTGGTCGAGGGGAAGGGGCCAGGTGCTGAG GAACCCGCACCCAGCAAAAATCCCTCTCCTGGCCAGGAGCTTCCTCCAGGACAAGACCTCCCTCCCAGCA AAGACCCCTCTCCCAGCCAGGAGCTTCCTGCAGGACAAGATCTCCCTCCCAGCAAAGACCCCTCTCCCAG CCAGGAGCTTCCTGCAGGACAAGATCTCCCTCCCAGCAAAGACCCCTCTCCCAGCCAGGAGCTTCCTGTA GGACAAGACCTCCCACCCAGGAAGGACTCCTCAGGCCAAGAAGCTGCTCCTGGCCCAGAATCACCATCCA GTGAAGACATAGCAACCTGCCCTAAGCCCCCTCAAAGCCCAGAAACCTCAACAAGCAAGGACTCCCCACC AGGCCAGGGATCCTCCCCGACCACAGAACTCCCATCTTGCCAGGGCCTTCCTGCTGGTCAAGAATCTACT AGCCAGGACCCTCTGCTCAGTCAAGAGCCCCCTGTTATCCCAGAATCCTCTGCCTCCGTCCAGAAACGCC TACCCTCTCAGGAGTCACCCTCCAGCCTGGGCTCCCTGCCAGAGAAGGACCTTGCTGAGCAGACCATCAG CTCCGGGGAGCCACCAGTCGCCACTGGTGCTGTACTGCCAGCCTCTAGACCTAACTTTGTGATCCCCGAG GTGCGTCTGGATAATGCCTACAGCCAACTGGATGGGGCCCACGGAGGCAGCTCTGGCGAGGACGAGGATG CAGAAGAGGGAGAGGAGGGGGGAGAAGGGGAGGAAGATGAGGAGGACGACACCAGCGACGACAACTACGG GGATCGTAGTGAGGCCAAGCGCAGCAGCCTGATTGAGACTGGCCAAGGCGCCGAGGGCGGCTTCTCGTTG CGTGTGCAGAACTCGCTGCGGCGCCGGACGCACAGCGAGGGCAGCCTGCTGCAGGAGTCCCGGGGGCCCT GCTTTGCCTCTGACACCACCTTACACTGCTCTGATGGCGAGGGCGCCACATCCACCTGGGCTATCCCTTC ACCCCGCACCCTCAAAAAAGAACTGGGTCGTAATGGAGGCTCCATGCACCACCTTTCCCTGTTCTTCACG GGACACAGGAAGATGAGTGGGACTGACCTCACAGAATGTGATGAAGCTTCCCGGAAGAGAAAGAGCAAAA ACATAGCCAAGGACATGAAGAATAAGCTGGCCATCTTCAGGCGGCGGAACGAATCTCCCGGGGCTCAGCC AGCCAGCAAGACAGACAAGACAACCAAGTCCTTCAAGCCTACCTCGGAGGAAGCCCTCAAGTGGAGCGAG TCCCTGGAAAAGTTGCTGCTTCATAAATATGGCTTAGAAGTGTTCCAGGCCTTCCTTCGCACCGAGTTCA GTGAGGAGAACCTGGAGTTTTGGCTCGCTTGCGAGGACTTCAAGAAGGTCAAATCACAGTCCAAGATGGC AGCCAAAGCCAAGAAGATCTTTGCTGAATTCATCGCAATCCAGGCTTGCAAAGAGGTAAACCTGGACTCC TACACACGAGAACACACCAAGGAGAATCTGCAGAGCATCACCCGAGGCTGCTTTGACTTGGCACAGAAAC GTATCTTTGGGCTCATGGAGAAGGACTCTTACCCTCGCTTCCTCCGCTCTGACCTCTACCTGGACCTCAT TAACCAGAAGAAGATGAGTCCCCCGCTCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_134257
Insert Size	2901 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_134257.3, NP_599018.3
RefSeq Size	3751 bp
RefSeq ORF	2901 bp
Locus ID	50780
UniProt ID	Q9DC04
Gene Summary	Down-regulates signaling from heterotrimeric G-proteins by increasing the GTPase activity of the alpha subunits, thereby driving them into their inactive GDP-bound form. Down-regulates G-protein-mediated release of inositol phosphates and activation of MAP kinases.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) encodes the longest isoform (2).

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