Myocd (NM_145136) Mouse Untagged Clone

CAT#: MC222964

Myocd (untagged) - Mouse myocardin (Myocd), transcript variant A, (10ug)



  "NM_145136" in other vectors (4)

CNY 12,640.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (3)
Rabbit Polyclonal Anti-Capn15 Antibody
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms BSAC2A; Srfcp
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222964 representing NM_145136
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACACTCCTGGGGTCTGAACACTCTTTGCTGATTAGAAGGAAGTTCCGATCAGTCTTACAGTTACGGC
TTCAACAGAGAAGGACCCAGGAGCAGCTGGCTAACCAAGGCTTAATACCGCCACTGAAAGGTCCAACTGA
ATTCCATGACCCGAGAAAACAATTGGATAGTGCCAAGACTGAAGATTCCCTGAGGCGCAAGGGCAGAAAC
AGGTCCGACCGTGCCAGCCTGGTTACTATGCACATTCTCCAAGCCTCCACGGCAGAAAGGTCCATTCCAA
CTGCTCAGATGAAGCTCAAAAGAGCCCGCCTTGCAGATGACCTCAATGAGAAGATCGCTCTCCGCCCAGG
GCCCTTGGAACTGGTGGAGAAGAACATTCTGCCGATGGATTCTTCCGTGAAAGAGGCTATAAAAGGTACT
GAGGTGAGCCTCTCCAAGGCAGCAGATGCATTCGCCTTTGAGGATGACAGCAGTAGAGATGGGCTCTCTC
CAGATCAGGCTAGGAGCGAGGACCCCCAGGGCTCTACAGGATCCACCCCAGACATCAAATCCACTGAGGC
TCCTCTGGACACAATCCAGGATCTCACTCCTGGCTCAGAAAGTGACAAGAATGATGCAGCCTCCCAGCCA
GGCAACCAGTCAGACCCTGGGAAGCAGGTTCTCGGCCCCCTCAGCACCCCGATTCCTGTGCACACTGCTG
TAAAGTCCAAGTCTTTGGGTGACAGTAAGAACCGCCACAAAAAGCCCAAAGACCCCAAACCAAAGGTGAA
GAAGCTCAAATACCATCAGTACATCCCCCCAGACCAGAAGGCAGAGAAGTCTCCCCCACCCATGGACTCT
GCCTATGCCCGGCTGCTCCAGCAACAGCAGCTATTCCTGCAGCTACAGATCCTCAGCCAGCAGCAGCAAC
AGCAGCAGCAACAGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAGCAGCGGTTCAGCTACCCTGGGAT
GCACCAAACACACCTCAAAGAACCAAATGAACAGATGGCCAGAAATCCGAATCCTTCTTCAACACCACTG
AGCAATACCCCTCTATCCCCTGTCAAAAATAGCATTTCTGGACAAACTGGTGTTTCTTCTCTCAAACCAG
GCCCCCTCCCACCCAACCTGGATGATCTCAAGGTGTCAGAGTTAAGACAACAGCTTCGAATCCGGGGCTT
GCCAGTGTCAGGCACCAAGACAGCGCTGGTGGACCGGCTTCGTCCCTTCCAGGATTGTGCTGGCAACCCT
GTGCCCAACTTTGGGGACATCACAACTGTCACCTTTCCTGTCACGCCCAACACCTTGCCCAGTTATCAGT
CCTCCCCGACAGGCTTCTACCACTTTGGCAGCACAAGCTCCAGCCCACCCATCTCCCCCGCCTCATCTGA
CTTGTCCGCTGCAGGGTCCCTGCCAGACACCTTCACCGATGCGTCACCTGGCTTCGGCCTGCACGCATCT
CCGGTGCCCGCCTGCACGGACGAGAGTCTGCTGAGCAGCCTGAATGGGGGCTCGGGCCCCTCCGAGCCTG
ATGGGCTAGACTCTGAGAAGGACAAGATGCTGGTGGAGAAGCAGAAAGTGATCAACCAGCTCACCTGGAA
GCTGCGGCAAGAGCAGCGGCAGGTGGAAGAGCTGAGAATGCAACTGCAGAAGCAGAAGAGCAGCTGCAGC
GACCAGAAGCCACTGCCCTTCTTGGCCACCACCATCAAACAGGAAGATGTCTCCAGCTGCCCCTTCGCAC
CCCAGCAGGCGTCTGGGAAGGGACAGGGCCACAGCTCTGACAGTCCCCCTCCGGCTTGTGAGACGGCTCA
GCTGCTGCCTCACTGTGTGGAGTCCTCAGGTCAAACCCATGTACTCTCGTCCACGTTTCTCAGCCCCCAG
TGCTCCCCTCAGCACTCGCCCCTGGGGGGCCTGAAGAGCCCGCAGCACATCAGCCTGCCTCCATCACCCA
ACAACCATTACTTCCTGGCTTCCTCTTCGGGAGCTCAGAGAGAGAACCATGGGGTCTCTTCACCCAGCAG
CAGCCAAGGGTGCGCACAGAACTCAGGGGCACACGAAGGCCATTCTTCTAGCTTCTCTTCCCCAGCTTCC
AGCCTCCATCAGCCTTTCTCTGGCACCCAAGCAGACAGCAGTCACAGTGCTGGGCTCAACCCTTGTCCCA
AAAGCCCAAGTATTCATCCAAAGATGACTGGTTTACAATCTTCTGACAAGGTGGGGCCAACGTTTTCAAT
TCCATCCCCAACTTTTTCTAAGTCAAGTTCAGCAGTTTCAGATATCACCCAGCCCCCATCCTATGAAGAT
GCAGTGAAGCAGCAAATGACTCGGAGTCAGCAGATGGACGAACTCCTGGATGTCCTCATTGAAAGTGGAG
AAATGCCAGCCGATGCCAGGGAAGATCATTCATGTCTTCAGAAAATTCCAAAGATCCCTGGGTCCTCCTG
CAGCCCAACTGCCATCCCCCCGAAGCCCTCGGCTTCCTTTGAGCAGGCATCTTCGGGAGGCCAGATGGCC
TTCGATCACTACGCCAACGACAGTGACGAACACCTGGAAGTCTTATTGAATTCTCACAGCCCCATCGGAA
AGGTGAGCGATGTTACCCTCCTCAAAATCGGAAGCGAGGAGCCTCCTTTTGACAGCATCATGGATGGCTT
CCCAGGGAAGGCTGCGGAAGATCTCTTCAGTGCTCACGAGCTCTTGCCTGGGCCCCTCTCCCCGATGCAT
GCACAGTTGTCACCTCCTTCTGTGGACAGCAGTGGTCTGCAGCTGAGCTTCACGGAATCTCCTTGGGAAA
CAATGGAATGGCTGGACCTCACTCCACCTAGTTCCACGCCAGGCTTCAGCAACCTTACCTCCAGTGGGCC
CAGCATTTTCAACATCGATTTTCTGGATGTTACAGATCTTAATCTGAATTCCCCTATGGATCTCCACTTA
CAGCAGTGGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_145136
Insert Size 2952 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_145136.4, NP_660118.3
RefSeq Size 5127 bp
RefSeq ORF 2952 bp
Locus ID 214384
UniProt ID Q8VIM5
Gene Summary Smooth muscle cells (SM) and cardiac muscle cells-specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage (myogenesis). Isoform 1 mediates the cardiac transcription factor MEF2C-dependent transcription. Isoform 1 and isoform 3 are more active than isoform 2 and isoform 4 in stimulating cardiac muscle promoters.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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