Ephb1 (NM_173447) Mouse Untagged Clone

CAT#: MC222976

Ephb1 (untagged) - Mouse Eph receptor B1 (Ephb1), transcript variant 1, (10ug)



  "NM_173447" in other vectors (4)

CNY 12,640.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-EphB1 (extracellular)
    • 50 ul

CNY 11,000.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 9330129L11; AW488255; C130099E04Rik; Cek6; Elk; Elkh; ENSMUSG00000074119; Hek6; Net
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222976 representing NM_173447
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCTGGATTGCTTGCTGCTCTTCCTCCTGGCATCTGCAGTGGCCGCGATGGAAGAGACATTGATGG
ACACAAGGACAGCCACTGCAGAGTTGGGATGGACGGCCAACCCTGCCTCTGGGTGGGAAGAAGTCAGTGG
CTATGATGAAAACCTGAACACCATCCGTACTTACCAAGTGTGCAACGTCTTCGAACCCAACCAGAACAAC
TGGCTGCTTACCACCTTTATCAACAGAAGGGGCGCCCATCGCATCTATACAGAGATGCGCTTCACTGTGA
GGGACTGCAGCAGCCTTCCAAATGTCCCAGGCTCCTGCAAGGAGACCTTCAACTTGTACTACTATGAGAC
TGACTCTGTGATTGCCACCAAGAAGTCAGCCTTCTGGTCTGAAGCCCCCTACCTCAAAGTGGACACCATT
GCTGCAGATGAGAGCTTCTCCCAGGTGGATTTTGGGGGAAGGTTGATGAAAGTCAACACGGAAGTCAGGA
GCTTTGGGCCTCTTACTAGGAACGGTTTTTACCTCGCTTTCCAGGATTATGGAGCCTGTATGTCTCTCCT
TTCTGTCCGTGTCTTCTTCAAAAAGTGTCCCAGCATCGTGCAGAATTTTGCAGTGTTCCCAGAAACCATG
ACAGGAGCAGAGAGCACATCTCTGGTGATTGCTCGGGGCACATGCATCCCAAATGCGGAAGAAGTGGATG
TGCCCATAAAACTCTACTGCAACGGAGATGGAGAGTGGATGGTGCCCATTGGGCGCTGTACCTGTAAGCC
TGGCTATGAGCCTGAGAACAGCGTGGCCTGCAAGGCCTGTCCTGCGGGGACCTTCAAGGCCAGCCAGGAA
GCTGAAGGCTGCTCCCACTGCCCCTCCAACAGTCGCTCCCCTTCAGAGGCGTCTCCCATCTGCACCTGCC
GGACTGGCTATTACCGAGCTGACTTTGATCCACCAGAGGTGGCGTGTACTAGTGTCCCATCGGGTCCTCG
AAATGTCATCTCCATCGTGAATGAGACATCTATCATTCTAGAGTGGCACCCTCCAAGAGAGACTGGTGGG
AGAGATGACGTGACGTACAACATCATCTGCAAGAAGTGCCGAGCAGACCGCCGCAGCTGCTCCCGCTGCG
ATGACAATGTGGAGTTTGTCCCCAGGCAACTGGGCTTGACTGAGTGTCGTGTCTCCATCAGTAGCCTATG
GGCCCACACCCCGTACACCTTTGATATCCAGGCCATCAATGGAGTCTCTAGCAAGAGTCCCTTTCCCCCA
CAGCACGTCTCTGTCAACATCACCACAAACCAAGCTGCCCCCTCCACTGTTCCTATCATGCACCAGGTCA
GTGCCACCATGAGGAGCATCACCTTGTCATGGCCTCAGCCGGAGCAACCCAATGGCATAATCCTGGACTA
TGAGATCCGGTACTATGAGAAGGAACACAATGAGTTCAACTCTTCCATGGCCAGGAGCCAGACCAACACA
GCACGTATCGATGGGCTACGGCCTGGCATGGTATACGTGGTCCAGGTGCGAGCTCGAACCGTGGCTGGCT
ATGGCAAGTTCAGTGGCAAGATGTGTTTCCAGACTCTGACAGATGATGATTACAAGTCGGAGCTGAGAGA
GCAGCTACCCCTGATTGCTGGCTCGGCAGCAGCTGGAGTCGTATTTGTTGTGTCTCTGGTGGCCATCTCT
ATTGTCTGCAGCAGGAAACGAGCTTACAGCAAAGAGGCTGCGTACAGTGATAAGCTTCAACATTACAGCA
CAGGCCGAGGCTCCCCAGGGATGAAGATCTACATTGACCCATTCACTTATGAGGACCCCAATGAAGCCGT
CCGGGAATTTGCCAAGGAGATTGATGTATCTTTTGTGAAAATTGAAGAGGTCATCGGAGCAGGGGAGTTC
GGCGAGGTGTACAAGGGCCGTTTGAAGCTGCCAGGCAAGAGGGAAATCTATGTGGCCATCAAGACCCTGA
AGGCTGGGTACTCAGAGAAACAGCGTCGGGATTTTCTGAGCGAGGCGAGCATCATGGGCCAGTTTGACCA
TCCCAACATCATTCGCCTGGAGGGTGTCGTCACCAAGAGCCGGCCTGTCATGATCATTACGGAGTTCATG
GAGAACGGCGCTTTAGACTCTTTCCTCCGGCAAAATGATGGACAGTTCACCGTGATCCAGCTTGTGGGGA
TGCTGAGGGGCATCGCTGCTGGCATGAAGTACCTATCTGAGATGAATTATGTGCACCGGGACCTGGCTGC
TAGAAACATTCTGGTCAACAGCAACCTGGTGTGCAAAGTTTCTGACTTTGGTCTCTCTCGCTACCTCCAG
GATGACACCTCAGACCCCACCTACACCAGCTCCTTGGGAGGGAAGATCCCTGTGAGATGGACAGCTCCAG
AGGCCATCGCCTACCGCAAGTTTACGTCAGCCAGCGATGTCTGGAGCTATGGGATTGTCATGTGGGAAGT
GATGTCATTTGGAGAGAGACCTTACTGGGATATGTCCAATCAAGATGTCATCAATGCCATTGAGCAGGAT
TACCGGCTGCCTCCTCCTATGGACTGCCCAGCTGCCCTGCACCAGCTCATGCTGGACTGTTGGCAGAAGG
ATCGCAATAGCCGGCCCCGTTTTGCAGAGATCGTCAACACCCTGGACAAGATGATCCGGAACCCAGCTAG
TCTCAAGACTGTGGCAACCATCACCGCTGTGCCTTCCCAACCCCTGCTTGACCGCTCTATCCCAGACTTC
ACGGCCTTTACCACCGTGGATGACTGGCTAAGTGCCATCAAAATGGTCCAGTACAGGGACAGCTTCCTCA
CCGCAGGCTTCACCTCCCTTCAGCTGGTCACCCAGATGACATCAGAAGACCTCCTGAGAATAGGGGTAAC
CTTGGCAGGCCATCAGAAGAAGATTCTGAGTAGCATTCACTCAATGAGGGTCCAGATGAACCAGTCACCA
TCGGTAATGGCGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_173447
Insert Size 2955 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_173447.3, NP_775623.3
RefSeq Size 4686 bp
RefSeq ORF 2955 bp
Locus ID 270190
UniProt ID Q8CBF3
Gene Summary Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Cognate/functional ephrin ligands for this receptor include EFNB1, EFNB2 and EFNB3. During nervous system development, regulates retinal axon guidance redirecting ipsilaterally ventrotemporal retinal ganglion cells axons at the optic chiasm midline. This probably requires repulsive interaction with EFNB2. In the adult nervous system together with EFNB3, regulates chemotaxis, proliferation and polarity of the hippocampus neural progenitors. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and synapse formation. May also regulate angiogenesis. More generally, may play a role in targeted cell migration and adhesion. Upon activation by EFNB1 and probably other ephrin-B ligands activates the MAPK/ERK and the JNK signaling cascades to regulate cell migration and adhesion respectively. Involved in the maintenance of the pool of satellite cells (muscle stem cells) by promoting their self-renewal and reducing their activation and differentiation (PubMed:27446912).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...