Phkb (NM_199446) Mouse Untagged Clone
CAT#: MC223468
Phkb (untagged) - Mouse phosphorylase kinase beta (Phkb), (10ug)
CNY 13,870.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | AI463271 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC223468 representing NM_199446
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAACTCACCTGATGCAGCCTTCTCTTCACCTGCTTTGTTAAGGTCTGGCTCAGTTTATGAACCTC TTAAAAGCATTAATCTTCCAAGACCTGATAATGAAACTCTATGGGATAAGTTGGATCATTATTACAGAAT TGTCAAGTCAACGATGCTGATGTATCAAAGTCCAACTACCGGTCTCTTTCCTACTAAAACATGTGGTGGA GAAGAGAAGTCCAAAGTGCACGAGAGTCTGTACTGTGCTGCGGGTGCCTGGGCCTTGGCGCTAGCATACA GGCGCATTGATGATGACAAGGGACGGACCCATGAGCTGGAGCACTCGGCCATAAAGTGTATGAGAGGAAT TCTCTACTGCTACATGCGGCAGGCTGATAAGGTCCAACAGTTTAAACAAGATCCCCGGCCCACAACATGT CTTCACTCTGTTTTCAGTGTGCACACTGGGGATGAGTTGCTCAGCTATGAAGAATATGGCCATCTTCAGA TAAATGCAGTCTCACTCTTTCTCCTTTACCTGGTAGAAATGATTTCCTCAGGCCTCCAGATAATCTATAA CACGGATGAGGTTTCTTTTATTCAAAACCTTGTATTTTGTGTAGAGAGAGTTTACCGTGTTCCTGACTTT GGTGTCTGGGAAAGAGGAAGCAAATATAACAATGGTAGTACCGAACTGCACTCAAGCTCTGTTGGTTTAG CAAAAGCAGCCTTAGAAGCAATTAATGGATTTAACCTCTTTGGAAACCAGGGGTGTTCCTGGTCAGTTAT ATTTGTGGATCTCGATGCTCACAACCGCAACAGGCAGACATTGTGCTCACTGTTACCCAGAGAATCACGC TCTCATAACACAGATGCTGCCCTGCTCCCGTGCATCAGTTACCCTGCGTTTGCCTTGGATGATGAAGCAC TGTTCAGCCAGACACTTGATAAAGTGATAAGAAAATTGAAAGGAAAATATGGATTTAAGCGTTTCTTGCG AGATGGGTACAGAACGCCATTGGAAGATCCCAACAGACGCTATTACAAACCAGCAGAGATTAAGCTGTTT GATGGCATTGAATGTGAATTTCCCATATTCTTCCTTTACATGATGATCGATGGTGTTTTTAGAGGCAATC TTGAACAAGTCAAGGAATATCAGGACCTTTTGACACCTTTGCTTCATCAGACCACAGAAGGATATCCTGT TGTGCCAAAGTACTATTATGTGCCAGCTGACTTTGTAGAATGTGAAAAAAGAAACCCTGGTAGTCAGAAG CGGTTTCCAAGTAACTGTGGCCGTGATGGGAAGTTGTTTCTTTGGGGACAAGCTCTTTATATCATTGCAA AACTCCTAGCTGATGAGTTAATCAGCCCTAAAGACATTGACCCTGTCCAGCGCTTTGTCCCACTTCAGAA TCAACGTAACGTGAGCATGAGATACTCCAATCAGGGCCCACTGGAAAATGACTTGGTGGTTCATGTAGCA CTTGTAGCAGAAAGCCAACGCCTTCAAGTTTTTCTCAATACATATGGTATTCAAACCCAAACTCCTCAGC AGGTGGAACCCATTCAGATCTGGCCTCAGCAGGAGCTTGTAAAAGCTTATTTCCACCTGGGAATCAATGA AAAGTTGGGTCTGTCTGGAAGGCCAGATAGGCCCATTGGCTGCCTTGGTACATCAAAGATTTATCGCATT CTAGGAAAGACTGTGGTTTGTTACCCTATCATCTTTGACCTGAGTGATTTCTACATGTCTCAGGACGTTC TGCTGCTCATAGATGACATAAAGAACGCACTACAGTTCATTAAGCAATATTGGAAAATGCATGGACGCCC ACTTTTTCTTGTTCTCATCCGAGAAGACAACATAAGAGGTAGCAGATTCAACCCAATATTAGACATGCTG GCAGCCTTTAAAAAGGGAATAATTGGAGGAGTCAAAGTTCATGTTGATCGTTTACAGACATTGATATCCG GAGCTGTAGTAGAGCAGCTTGACTTTCTACGAATTAGTGACACAGAAAAGCTTCCAGAATTTAAGAGCTT TGAAGAACTAGAATTTCCTAAGCATTCAAAAGTCAAACGGCAGAGCAGTACCGCAGATGCTCCTGAGGCG CAACACGAGCCAGGCATCACCATTACCGAGTGGAAAAACAAATCCACCCACGAAATCCTTCAGAAGCTGA ATGACTGCGGTTGTCTGGCTGGTCAAACCATCCTGCTGGGTATACTGCTCAAAAGAGAGGGCCCTAACTT TATCACAATGGAAGGAACTGTGTCTGATCACATTGAGAGAGTGTATAGAAGAGCTGGCAGCAAAAAGCTT TGGTCGGTTGTACGCCGTGCAGCAAGTCTTTTAAATAAAGTAGTGGACAGCCTGGCCCCATCCATTACTA ATGTTTTAGTGCAGGGCAAACAGGTAACTCTAGGTGCCTTTGGACATGAAGAGGAGGTCATCTCTAACCC ACTGTCTCCAAGAGTGATTAAGAACATAATCTATTATAAGTGCAATACCCACGATGAGAGGGAGGCAGTC ATTCAGCAAGAACTGGTCATCCACATCGGCTGGATCATCTCCAACAGCCCTGAGTTGTTCAGCGGCATGC TGAAAATACGCATTGGGTGGATCATCCACGCCATGGAGTATGAACTGCAGGTCCGGGGCGGGGATAAGCC AGCTGTGGACTTGTACCAGCTCTCGCCCAGTGAAGTGAAGCAGCTGCTGCTAGACATTCTCCAGCCTCAA CAGAGTGGAAGATGTTGGTTGAATAGACGTCAGATTGATGGGTCATTGAATAGAACACCCCCGGAATTCT ATGACCGAGTGTGGCAGATCCTGGAACGCACACCTAATGGTATCGTTGTAGCTGGAAAGCATTTGCCACA GCAACCAACTTTGTCGGATATGACCATGTATGAGATGAATTTTTCTCTCCTTGTTGAAGATATGCTGGGA AACATTGACCAGCCAAAGTATAGACAGATCATAGTTGAGTTACTAATGGTCGTATCCATTGTGCTGGAAA GAAACCCTGAACTGGAGTTTCAAGACAAAGTGGATCTGGACAGACTGGTTAAAGAAGCGTTTCATGAGTT TCAAAAAGATGAGAGTCGGCTAAAAGAAATCGAAAAACAAGATGATATGACTTCCTTTTACAACACGCCC CCACTGGGAAAAAGAGGAACCTGCAGCTACTTGACGAAGGTTGTGATGAACTCACTGCTCGAGGGAGAAG TCAAGCCCAGCAATGAGGACTCCTGCCTGGTTAGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_199446 |
Insert Size | 3258 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_199446.1, NP_955517.1 |
RefSeq Size | 4640 bp |
RefSeq ORF | 3258 bp |
Locus ID | 102093 |
UniProt ID | Q7TSH2 |
Gene Summary | Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG211607 | Phkb (tGFP-tagged) - Mouse phosphorylase kinase beta (Phkb) |
CNY 9,220.00 |
|
MR211607 | Phkb (Myc-DDK-tagged) - Mouse phosphorylase kinase beta (Phkb) |
CNY 7,552.00 |
|
MR211607L3 | Lenti ORF clone of Phkb (Myc-DDK-tagged) - Mouse phosphorylase kinase beta (Phkb) |
CNY 10,260.00 |
|
MR211607L4 | Lenti ORF clone of Phkb (mGFP-tagged) - Mouse phosphorylase kinase beta (Phkb) |
CNY 10,260.00 |