United States
Germany
Japan
United Kingdom
China
Phkb (NM_199446) Mouse Tagged ORF Clone
CAT＃: MG211607
- TrueORF®
Phkb (tGFP-tagged) - Mouse phosphorylase kinase beta (Phkb)
ORF Plasmid: DDK
"NM_199446" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 9,220.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI463271 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211607 representing NM_199446
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAACTCACCTGATGCAGCCTTCTCTTCACCTGCTTTGTTAAGGTCTGGCTCAGTTTATGAACCTC TTAAAAGCATTAATCTTCCAAGACCTGATAATGAAACTCTATGGGATAAGTTGGATCATTATTACAGAAT TGTCAAGTCAACGATGCTGATGTATCAAAGTCCAACTACCGGTCTCTTTCCTACTAAAACATGTGGTGGA GAAGAGAAGTCCAAAGTGCACGAGAGTCTGTACTGTGCTGCGGGTGCCTGGGCCTTGGCGCTAGCATACA GGCGCATTGATGATGACAAGGGACGGACCCATGAGCTGGAGCACTCGGCCATAAAGTGTATGAGAGGAAT TCTCTACTGCTACATGCGGCAGGCTGATAAGGTCCAACAGTTTAAACAAGATCCCCGGCCCACAACATGT CTTCACTCTGTTTTCAGTGTGCACACTGGGGATGAGTTGCTCAGCTATGAAGAATATGGCCATCTTCAGA TAAATGCAGTCTCACTCTTTCTCCTTTACCTGGTAGAAATGATTTCCTCAGGCCTCCAGATAATCTATAA CACGGATGAGGTTTCTTTTATTCAAAACCTTGTATTTTGTGTAGAGAGAGTTTACCGTGTTCCTGACTTT GGTGTCTGGGAAAGAGGAAGCAAATATAACAATGGTAGTACCGAACTGCACTCAAGCTCTGTTGGTTTAG CAAAAGCAGCCTTAGAAGCAATTAATGGATTTAACCTCTTTGGAAACCAGGGGTGTTCCTGGTCAGTTAT ATTTGTGGATCTCGATGCTCACAACCGCAACAGGCAGACATTGTGCTCACTGTTACCCAGAGAATCACGC TCTCATAACACAGATGCTGCCCTGCTCCCGTGCATCAGTTACCCTGCGTTTGCCTTGGATGATGAAGCAC TGTTCAGCCAGACACTTGATAAAGTGATAAGAAAATTGAAAGGAAAATATGGATTTAAGCGTTTCTTGCG AGATGGGTACAGAACGCCATTGGAAGATCCCAACAGACGCTATTACAAACCAGCAGAGATTAAGCTGTTT GATGGCATTGAATGTGAATTTCCCATATTCTTCCTTTACATGATGATCGATGGTGTTTTTAGAGGCAATC TTGAACAAGTCAAGGAATATCAGGACCTTTTGACACCTTTGCTTCATCAGACCACAGAAGGATATCCTGT TGTGCCAAAGTACTATTATGTGCCAGCTGACTTTGTAGAATGTGAAAAAAGAAACCCTGGTAGTCAGAAG CGGTTTCCAAGTAACTGTGGCCGTGATGGGAAGTTGTTTCTTTGGGGACAAGCTCTTTATATCATTGCAA AACTCCTAGCTGATGAGTTAATCAGCCCTAAAGACATTGACCCTGTCCAGCGCTTTGTCCCACTTCAGAA TCAACGTAACGTGAGCATGAGATACTCCAATCAGGGCCCACTGGAAAATGACTTGGTGGTTCATGTAGCA CTTGTAGCAGAAAGCCAACGCCTTCAAGTTTTTCTCAATACATATGGTATTCAAACCCAAACTCCTCAGC AGGTGGAACCCATTCAGATCTGGCCTCAGCAGGAGCTTGTAAAAGCTTATTTCCACCTGGGAATCAATGA AAAGTTGGGTCTGTCTGGAAGGCCAGATAGGCCCATTGGCTGCCTTGGTACATCAAAGATTTATCGCATT CTAGGAAAGACTGTGGTTTGTTACCCTATCATCTTTGACCTGAGTGATTTCTACATGTCTCAGGACGTTC TGCTGCTCATAGATGACATAAAGAACGCACTACAGTTCATTAAGCAATATTGGAAAATGCATGGACGCCC ACTTTTTCTTGTTCTCATCCGAGAAGACAACATAAGAGGTAGCAGATTCAACCCAATATTAGACATGCTG GCAGCCTTTAAAAAGGGAATAATTGGAGGAGTCAAAGTTCATGTTGATCGTTTACAGACATTGATATCCG GAGCTGTAGTAGAGCAGCTTGACTTTCTACGAATTAGTGACACAGAAAAGCTTCCAGAATTTAAGAGCTT TGAAGAACTAGAATTTCCTAAGCATTCAAAAGTCAAACGGCAGAGCAGTACCGCAGATGCTCCTGAGGCG CAACACGAGCCAGGCATCACCATTACCGAGTGGAAAAACAAATCCACCCACGAAATCCTTCAGAAGCTGA ATGACTGCGGTTGTCTGGCTGGTCAAACCATCCTGCTGGGTATACTGCTCAAAAGAGAGGGCCCTAACTT TATCACAATGGAAGGAACTGTGTCTGATCACATTGAGAGAGTGTATAGAAGAGCTGGCAGCAAAAAGCTT TGGTCGGTTGTACGCCGTGCAGCAAGTCTTTTAAATAAAGTAGTGGACAGCCTGGCCCCATCCATTACTA ATGTTTTAGTGCAGGGCAAACAGGTAACTCTAGGTGCCTTTGGACATGAAGAGGAGGTCATCTCTAACCC ACTGTCTCCAAGAGTGATTAAGAACATAATCTATTATAAGTGCAATACCCACGATGAGAGGGAGGCAGTC ATTCAGCAAGAACTGGTCATCCACATCGGCTGGATCATCTCCAACAGCCCTGAGTTGTTCAGCGGCATGC TGAAAATACGCATTGGGTGGATCATCCACGCCATGGAGTATGAACTGCAGGTCCGGGGCGGGGATAAGCC AGCTGTGGACTTGTACCAGCTCTCGCCCAGTGAAGTGAAGCAGCTGCTGCTAGACATTCTCCAGCCTCAA CAGAGTGGAAGATGTTGGTTGAATAGACGTCAGATTGATGGGTCATTGAATAGAACACCCCCGGAATTCT ATGACCGAGTGTGGCAGATCCTGGAACGCACACCTAATGGTATCGTTGTAGCTGGAAAGCATTTGCCACA GCAACCAACTTTGTCGGATATGACCATGTATGAGATGAATTTTTCTCTCCTTGTTGAAGATATGCTGGGA AACATTGACCAGCCAAAGTATAGACAGATCATAGTTGAGTTACTAATGGTCGTATCCATTGTGCTGGAAA GAAACCCTGAACTGGAGTTTCAAGACAAAGTGGATCTGGACAGACTGGTTAAAGAAGCGTTTCATGAGTT TCAAAAAGATGAGAGTCGGCTAAAAGAAATCGAAAAACAAGATGATATGACTTCCTTTTACAACACGCCC CCACTGGGAAAAAGAGGAACCTGCAGCTACTTGACGAAGGTTGTGATGAACTCACTGCTCGAGGGAGAAG TCAAGCCCAGCAATGAGGACTCCTGCCTGGTTAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211607 representing NM_199446
Red=Cloning site Green=Tags(s) MANSPDAAFSSPALLRSGSVYEPLKSINLPRPDNETLWDKLDHYYRIVKSTMLMYQSPTTGLFPTKTCGG EEKSKVHESLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPTTC LHSVFSVHTGDELLSYEEYGHLQINAVSLFLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPDF GVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRESR SHNTDAALLPCISYPAFALDDEALFSQTLDKVIRKLKGKYGFKRFLRDGYRTPLEDPNRRYYKPAEIKLF DGIECEFPIFFLYMMIDGVFRGNLEQVKEYQDLLTPLLHQTTEGYPVVPKYYYVPADFVECEKRNPGSQK RFPSNCGRDGKLFLWGQALYIIAKLLADELISPKDIDPVQRFVPLQNQRNVSMRYSNQGPLENDLVVHVA LVAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYFHLGINEKLGLSGRPDRPIGCLGTSKIYRI LGKTVVCYPIIFDLSDFYMSQDVLLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDML AAFKKGIIGGVKVHVDRLQTLISGAVVEQLDFLRISDTEKLPEFKSFEELEFPKHSKVKRQSSTADAPEA QHEPGITITEWKNKSTHEILQKLNDCGCLAGQTILLGILLKREGPNFITMEGTVSDHIERVYRRAGSKKL WSVVRRAASLLNKVVDSLAPSITNVLVQGKQVTLGAFGHEEEVISNPLSPRVIKNIIYYKCNTHDEREAV IQQELVIHIGWIISNSPELFSGMLKIRIGWIIHAMEYELQVRGGDKPAVDLYQLSPSEVKQLLLDILQPQ QSGRCWLNRRQIDGSLNRTPPEFYDRVWQILERTPNGIVVAGKHLPQQPTLSDMTMYEMNFSLLVEDMLG NIDQPKYRQIIVELLMVVSIVLERNPELEFQDKVDLDRLVKEAFHEFQKDESRLKEIEKQDDMTSFYNTP PLGKRGTCSYLTKVVMNSLLEGEVKPSNEDSCLVS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_199446 |
| ORF Size | 3255 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_199446.2 |
| RefSeq Size | 4640 bp |
| RefSeq ORF | 3258 bp |
| Locus ID | 102093 |
| UniProt ID | Q7TSH2 |
| Gene Summary | Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The beta chain acts as a regulatory unit and modulates the activity of the holoenzyme in response to phosphorylation (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223468 | Phkb (untagged) - Mouse phosphorylase kinase beta (Phkb), (10ug) |
CNY 13,870.00 |
|
| MR211607 | Phkb (Myc-DDK-tagged) - Mouse phosphorylase kinase beta (Phkb) |
CNY 7,552.00 |
|
| MR211607L3 | Lenti ORF clone of Phkb (Myc-DDK-tagged) - Mouse phosphorylase kinase beta (Phkb) |
CNY 10,260.00 |
|
| MR211607L4 | Lenti ORF clone of Phkb (mGFP-tagged) - Mouse phosphorylase kinase beta (Phkb) |
CNY 10,260.00 |
