Depdc5 (NM_177786) Mouse Untagged Clone
CAT#: MC223497
Depdc5 (untagged) - Mouse DEP domain containing 5 (Depdc5), transcript variant 2, (10ug)
CNY 13,870.00
Product images
![](https://cdn.origene.com/img/defaults-img.jpg)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | AV016528 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC223497 representing NM_177786
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAACCACAAAGGTCTACAAACTTGTCATCCACAAGAAGGGCTTTGGGGGCAGTGATGATGAGCTAG TCGTGAACCCTAAAGTATTTCCTCACATCAAACTTGGAGACATTGTGGAGATTGCTCACCCCAATGATGA GTACAGTCCTTTGCTTTTGCAAGTCAAGTCGCTTAAGGAAGATTTACAGAAAGAAACTATCAGTGTGGAC CAGACTGTGACTCAAGTATTCCGGCTAAGACCTTATCAAGATGTCTATGTGAATGTTGTAGACCCCAAGG ATGTGACTCTTGACCTGGTGGAATTGACTTTTAAGGATCAGTATATTGGCCGTGGGGATATGTGGCGACT AAAGAAAAGTTTGGTGAGCACCTGTGCCTATATCACTCAAAAAGTGGAATTTGCTGGCATCAGGGCACAG GCTGGTGAGCTGTGGGTCAAGAATGAGAAGGTCATGTGTGGTTACATTAGTGAAGAGACCAGGGTGGTGT TCCGTTCTACGTCGGCTATGGTTTACATATTTATTCAGATGAGCTGTGAAATGTGGGATTTTGATATTTA TGGGGATCTGTACTTTGAGAAAGCTGTGAACGGTTTCCTCGCCGACCTGTTTACTAAATGGAAGGAGAAG AACTGTAGTCATGAAGTGACTGTGGTCCTGTTTTCCAGAACTTTCTATGATGCAAAATCTATTGATGAAT TTCCTGAAATAAACCGAGCTTCAATTCAAGAGGATCACAAAGGGAGATTCTATGAGGACTTTTACAAAGT GGTGGTGCAGAATGAGAGAAGGGAAGAGTGGACTTCACTCCTCGTGACCATTAAAAAACTCTTCATCCAG TATCCAGTGTTGGTGCGACTGGAACAGGCAGGGGGCTTTCCTCAAGGAGACAATTCTACCTCAGCACAAG GAAACTACCTAGAGGCCATCAACTTGTCATTCAATGTGTTTGACAAGCACTACATCAACCGAAACTTTGA CCGAACTGGGCAGATGTCTGTGGTGATCACGCCGGGGGTGGGTGTCTTTGAAGTGGACCGCCTACTCATG ATCTTGACCAAGCAGCGGATGATTGATAATGGAATTGGTGTGGACTTAGTGTGCATGGGAGAGCAGCCAC TACATGCTGTGCCATTATTCAAGCTACATAACCGGAGTGTTCCGAGGGATTCTCGACTGGGTGATGATTA TAATATTCCTCACTGGATAAACCATAGTTTCTACACATCCAAAAGCCAGCTCTTTTGTAACAGTTTCACT CCACGGATAAAACTAGCAGGAAAGAAGTCTGCCTCTGAGAAAACCAAAAATGGTCGTGATACATCTCTCG GGACTCCAAAGGAATCTGAAAACACCCTTCCCATCCAAGTAGATTATGATGCCTACGATGCTCAAGTGTT CAGGCTGCCTGGCCCATCCCGGGCTCAGCGCCTCGCCACCTGCAGGTCTGTGAGAGAACAGGAGAATCAC AGTCGCAAAAGTGCAAGCTCCTGTGATGTCTCATCCAGCCCTTCCCTGCCAAGCCGTGCACTGCCCACTG AGGAAGTGAGGAGCCAGGCTTCTGATGACAGCTCCCTGGGCAAGAGCACCAACATCCTGATGATCCCTAA CCCCCACCTGCACCAGTATGAAGTCAGCAGCTCACTGGGCTACACCAGCACCCGAGATGTCCTGGAGAAC ATGATAGAACCCCCACAGAGGGACTCCAGTGCACCAGGAAGGTTCCATGTGGGTAGTGCAGAGTCCATGC TCCATGTCCGACCTGGAGGATACACGCCTCAGAGAGCGCTGATTAACCCCTTCGCCCCCTCGAGGATGCC CATGAAGCTCACCTCCAACAGAAGGCGCTGGATGCACACTTTTCCTGTAGGCCCATCTGGAGAGGCCATC CAGATCCATCATCAGACCCGGCAAAACATGGCAGAGCTGCAGGGCAGCAGGCAGAGGGACCCTACCCACT CCTCTGCAGAGCTGTTGGAGTTGGCATACCATGAAGCTGCCGGGAGGCACAGCACTTCCCGCCAGCCTGG TGACAGCATGTCCTTGAACTTCAGTGGAACGGAAGAGCTTTCTGTCAGCCTGCTTAGCAACAGCAGTACA GGTGTGAATCCTAGGACCCAGAACAAGGACTCCCTAGAGGACAGTGTTTCTACCTCTCCAGACCCAATGC CAGGCTTCTGTTGCACAGTTGGAGTGGATTGGAAGTCTCTTACTACTCCTGCATGCCTTCCCCTCACCAC TGACTACTTCCCGGACCGTCAGGGCCTGCAGAATGACTACACAGAGGGCTGCTATGATCTCCTCCCAGAA GCAGACATGGACAGGAGGGATGAGGAGGGTGTTCAAATGACAGCCCAGCAAGTGTTTGAAGAGTTCATCT GCCAGCGTCTCATGCAGGGTTACCAAATCATAGTACAGCCAAAGACCCAGAAACCCAACACCACAGTCCC ACCCCCATTGAGCAGTAGCCCACTCTATAGCAGAGGCCTTGTGTCCCGAAATCGCCCTGAGGAGGAAGGC CAGTATTGGTTAAGTATGGGCAGAACTTTCCATAAGGTGACACTCAAGGACAAGATGATCACAGTAACTC GTTACCTTCCCAAGTACCCTTATGAATCTGCCCAGATCCATTACACCTACAGCCTCTGCCCTTCCCACTC TGACTCGGAGTTTGTCTCCTGTTGGGTGGATTTCTGCCACGAACGGCTCGAGGAATACAAGTGGAATTAC TTAGATCAGTATATTTGTTCTGCTGGCTCTGAAGACTTCAGCTTAATCGAGTCTCTGAAGTTCTGGAGAA CCCGATTTTTACTACTGCCAGCCTGTGTTACTGCCACCAAGCGCATCACAGAAGGGGAGGTGCACTGTGA CATCTATGGGGACAAACCCCGTGCAGATGAAGATGAGTGGCAGCTTCTAGATGGCTTTATTCGCTTTGTA GAGGGCTTAAATCGCATCCGCAGGCGCCACCGCTCAGACCGCATGATTCGGAAGGGGACTGCCATGAAAG GCTTGCAGATGACCGGGCCCATCTCTGCACACTCTCTCGAGGCAGCAGGCCCTCCAGTCGGCAAGAAAGG AACCTCAGCTCTGTCTGCCCTGCTGGAGATGGAAGCTAGTCAGAAGAGCCTAGGAGAACAGCAGACGACT GTGCACGGAAAAAGCTCCACTCAGCCAGCTGAGAACAGCAGTGTTGCCATGACTCCCACCTATGTGGACA GCCCACGCAAGGTGCAGCATGTGACCCGCACAGACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_177786 |
Insert Size | 3258 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_177786.4, NP_808454.2 |
RefSeq Size | 3569 bp |
RefSeq ORF | 3258 bp |
Locus ID | 277854 |
Gene Summary | As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, 3' UTR, and coding region, compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG215873 | Depdc5 (tGFP-tagged) - Mouse DEP domain containing 5 (Depdc5) transcript variant 2, (10ug) |
CNY 9,220.00 |
|
MR215873 | Depdc5 (Myc-DDK-tagged) - Mouse DEP domain containing 5 (Depdc5), transcript variant 2 |
CNY 7,552.00 |
|
MR215873L3 | Lenti ORF clone of Depdc5 (Myc-DDK-tagged) - Mouse DEP domain containing 5 (Depdc5), transcript variant 2 |
CNY 10,260.00 |
|
MR215873L4 | Lenti ORF clone of Depdc5 (mGFP-tagged) - Mouse DEP domain containing 5 (Depdc5), transcript variant 2 |
CNY 10,260.00 |